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The relation between individual trait differences, social mobility and social structure is central to social biology. Because genetic variance underlies phenotypic variance in some of these traits, for example IQ, several mechanisms determine the population variance. Polygenic inheritance is the basic mechanism. Social mobility and assortative partner choice distribute the trait variance within generations. This feedback circle is constrained by sociological conditions at several levels of analysis. Fundamental to this theory of social assortment is the relation between social-biological traits and social class on the one hand, and these traits and social mobility on the other hand. The focus here is on the relation between social class, social mobility and cognitive ability. The National Child Development Study is drawn upon, including the last follow-up (1999-2000). By approaching this relationship through various methods, both social-biological and sociological aspects of this research question can be assessed.

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A growing number of people is subject to age-related cognitive impairment due to the proportional increase of the ageing population. Therefore, there is a growing interest in cognition-enhancing substances. The efficacy of an alcohol/water extract of Ginkgo biloba in elderly individuals with memory- and/or concentration complaints was tested in a randomized, double-blind, placebo-controlled study by using both subjective and objective parameters. After a wash-out period of 4 weeks 241 non-institutionalised patients in the age range 55-86 years were randomly allocated to receive either Ginkgo biloba alcohol/water extract in a high dose (HD), a low dose (LD) or a placebo (PL) for 24 weeks. Patients were assessed using a psychometric testbattery in the following order: Expended Mental Control Test (EMCT) measuring attention and concentration, Benton Test of Visual Retention-Revised (measures short term visual memory), Rey Test part 1 (measures short term memory and learning curve), Beck Depressive Inventory (BDI) measuring the presence and severeness of a depression in order to exclude depressive patients and Rey Test part 2 (measures long term memory: recognition). Furthermore, subjective perception of memory and concentration was measured. 197 patients completed the study (mean MMSE score: 26.29). In the subjective test, the EMCT, the Rey 1 and Rey 2 no significant differences in improvement in time between the groups were observed. In the Benton test increases of 18%, 26% and 11% (expressed as percentage of baseline scores) were observed in the HD, LD and PL respectively (MANOVA; p = 0.0076). No substantial correlation was observed between subjective perception of the severeness of memory complaints and the objective test results. No differences in the number of (gastrointestinal) side effects were observed between placebo and verum groups. These results indicate that the use of Ginkgo extracts in elderly individuals with cognitive impairment might be promising. Further research using both subjective and objective measurements is recommended.

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OBJECTIVES: Since the proportion of elderly people with an insufficient intake of nutrients is high and many of the elderly have poor odour perception or poor dental state, in this study, the relation between age, odour perception, dental state and nutrient intake is explored. DESIGN: Single centre cross sectional study. SETTING: Independently living elderly were tested at their homes in 4 locations in Belgium, ranging in ages 60-90 y. SUBJECTS: 200 elderly participated in a 7 d food record study, resulting in 119 complete records. INTERVENTIONS: Food quantities were converted to nutrient intake levels. For all people, odour detection threshold was determined of isoamylacetate and dental status was noted. Path analysis was used and the separate effects of age, dental state, odour perception and gender were tested on macronutrient intake and micronutrient intake respectively. MAIN OUTCOME MEASURES: For all nutrients, no significant correlation was observed between nutrient intake and odour perception, except for energy, water, Fe and niacin (P < 0.05). A significant separate effect of odour perception was observed for water intake (CR = 2.09). Significant separate effects of dental state were observed for animal protein (CR = 2.29), niacine (CR = 2.04) and mono-unsaturated fats (CR = 2.32). CONCLUSIONS: Although odour perception and dental state can not fully explain variability in nutrient intake, our results show that people with poor odour perception have lower nutrient intake levels than people with good odour perception. Dental state may not be a direct cause of poor nutrition but a contribution factor in those elderly who have other risk factors.

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The effect of determinants of growth in body length from birth to 6 years of age were studied in a longitudinal sample of 59 male and 70 female infants from Lublin, Poland. Structural equation modeling (SEM) was used to study the effects of gender of the child, occupation of the parents, the educational level of the parents, per capita income, the stature of the parents, and the weight of the mother on body length at birth and at 1, 2, 3, and 6 years of age. Significant sex differences in length were observed at birth and during the first 2 years of postnatal life, but not in the period between 3 and 6 years of age. Socioeconomic status (SES), expressed as a latent variable in the SEM, was not related to body length at birth but was significantly related to body length during infancy and, to a lesser extent, to body length during childhood. Paternal stature was not related to body length at birth and during infancy, but was significantly related to body length from 3 years onwards. Maternal stature was significantly related to body length at birth and at 1 year of age, but not thereafter, while maternal weight was significantly related to body length at birth only. © 1996 Wiley-Liss, Inc.

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The effect of age, gender and phenobarbital treatment on the hepatic cytosolic glutathione S-transferase subunit composition was studied in Brown Norway rats. Affinity chromatography followed by reversed phase HPLC was used in order to separate the various glutathione S-transferase subunits. Corresponding steady-state mRNA levels were measured by Northern Blot analysis using cDNA clones hybridizing to mRNA encoding glutathione S-transferase subunits 1/2, 3/4 and 7, respectively. In all the age groups studied (15, 25, 53, 99, 112 and 136 weeks) the total amount of glutathione S-transferase protein was in untreated rats significantly higher in males (132 micrograms/mg cytosolic protein) than in females (91 micrograms/mg cytosolic protein) and significant gender dependent differences in the subunit composition were demonstrated. Aging seemed to be of minor importance in untreated as well as in phenobarbital treated rats. Under control conditions, the subunit composition of male rats between 15 and 136 weeks old consisted of 28, 12, 11 and 49% of subunits 1, 2, 3 and 4 respectively and of female animals of the same age groups of 38, 26, 7 and 30%, respectively. In all the age groups studied phenobarbital administration (45 mg/kg body weight, i.p., once a day for 7 days) doubled total glutathione S-transferase protein in both genders and affected the subunit composition in a significant way, emphasizing the already existing differences between genders. Subunits 1, 2 and 3, especially, were increased in male rats in comparison to females resulting in the observation that levels of glutathione S-transferase subunits studied became higher in males than in their female counterparts. The HPLC results were confirmed by steady-state mRNA analysis. In untreated rats, higher levels of mRNA encoding glutathione S-transferase subunits 1/2 and 3/4 were present in male than in female livers. Phenobarbital treatment increased mRNA levels in both genders. Subunit 7 was never detected. These effects were demonstrated in both young and old rats.

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The use of cultured human epithelium for skin grafting has recently been developed and used successfully to treat burns and various smaller skin defects. Cultured epidermal allografts are replaced by the recipient's own skin. The use of cultured allografts has a healing effect in chronic ulcerations and in burn wounds, probably by releasing growth factors. We have recently demonstrated that cryopreserved allografts can also induce wound healing. Both an edge effect and complete healing were found in ulcers treated with fresh or cryopreserved cultured epidermal allografts. These findings could be important in patient care, since in wound-healing problems, cryopreserved allografts could be immediately available. The cryopreservation of human keratinocyte sheets may also open new perspectives in pharmacotoxicological research on topically applied substances.

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The aim of this study was to measure the activity of phase I and II key enzymes in the biotransformation of xenobiotics and their inducibility by phenobarbital (2 mM) in two currently used in vitro models, namely adult rat hepatocytes, conventionally cultured or co-cultured with rat epithelial cells derived from primitive biliary duct cells. For phase I, the cytochrome P450 content and the enzymic activities of 7-ethoxycoumarin O-deethylase and aldrin epoxidase have been determined, for phase II glutathione S-transferase activity was measured. In conventional cultures, all phase I parameters investigated declined continuously as a function of culture time. Two mM phenobarbital had inducing effects on 7-ethoxycoumarin O-deethylase and glutathione S-transferases but not on aldrin epoxidase. In co-cultures, after an initial decrease, a steady state situation developed for all the parameters measured, lasting for at least 10 days. The cytochrome P450 content, the 7-ethoxycoumarin O-deethylase, aldrin epoxidase and glutathione S-transferase activities were maintained from 3 to 4 days on at 25, 100, 15 and 50%, respectively, of their corresponding value obtained for freshly isolated hepatocytes. After phenobarbital treatment, the parameters mentioned were significantly increased with the exception of the aldrin epoxidase activity of which the inducibility was nearly completely lost.

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Age-associated alterations of hepatic cytosolic glutathione S-transferase activities towards 1-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene were investigated in Brown Norway rats of both sexes (11-144 weeks old), under control conditions and after administration of phenobarbital. With both substrates, small changes in glutathione S-transferase activities are observed for the control rats (15-53 weeks old). For these specific age groups, male glutathione S-transferase activities are significantly higher than those of their female counterparts, with sex-related differences being most pronounced after 1,2-dichloro-4-nitrobenzene conjugation. Using 1-chloro-2,4-dinitrobenzene as a 'general' substrate, the sex-differences tend to decrease from the age of 53 weeks onwards to become non-significant at the age of 112 weeks. Phenobarbital administration significantly increases the total and the isoenzymes 3-3 and 3-4 activities in both sexes, with the highest and the lowest increase being observed in the youngest and oldest animals, respectively. It therefore can be concluded that some age-related variations exist as far as the glutathione S-transferase activity of both control and phenobarbital-treated rats are concerned, but that the changes observed are rather small. On the contrary, the parameters 'Sex' and 'Phenobarbital treatment' are found to be responsible for the major activity changes observed.

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