RESUMEN
Nanoparticles, such as TiO2 particles, have a great potential for biomedical applications due to their ultra-small size and large specific surface area. However, their detection within cells is to date more than challenging. Thus, implementing fluorescence properties to nanoparticles via their controlled functionalisation with an organic chromophore is an original and efficient strategy to enable their visualization. In this work, a silylated coupling agent bearing a luminescent rhodamine B group was synthesised and grafted on the surface of anatase nanoparticles. The successful functionalisation was demonstrated via zeta potential, dynamic light scattering and diffuse reflectance infrared Fourier transform analyses. Remarkably, the obtained luminescent TiO2 particles showed an improved photocatalytic activity compared to the pristine nanoparticles. Both, as-synthesised and functionalised TiO2 nanoparticles samples appear to be non-toxic towards malignant and non-malignant cells. Moreover, the detection of the functionalised particles within cultured cells was proven to be easy and efficient via confocal fluorescence microscopy.
Asunto(s)
Fluorescencia , Nanopartículas/química , Compuestos de Organosilicio/química , Procesos Fotoquímicos , Titanio/química , Investigación Biomédica , Catálisis , Microscopía Confocal , Microscopía Fluorescente , Compuestos de Organosilicio/síntesis química , Tamaño de la Partícula , Rodaminas/química , Propiedades de SuperficieRESUMEN
The use of dendritic structures for the grafting of core-shell γ-Fe(2)O(3)/polymer 300 nm superparamagnetic nanoparticles (MNPs) has been performed with four metallodendrons that were functionalized with diphosphinopalladium complexes. The catalytic performance of these nanocatalysts was optimized for the Suzuki C-C cross-coupling reaction. These results demonstrated the importance of optimizing the catalytic efficiency of grafted MNPs by optimizing the dendritic structures and the nature of the peripheral phosphine ligands. All of these nanocatalysts showed remarkable reactivity towards bromoarenes and they were recovered and efficiently reused by magnetic separation with almost no loss of reactivity, even after 25 cycles.