RESUMEN
For the first time substantiated was the role of the phenomenon of Yersinia pestis biofilm--extracellular matrix envelope (EME)--formation as the basis that determines the nature of plague agent interaction with flea organism. Implication of vector's proventriculus in the process of biofilm formation was demonstrated. Ultrastucture of plague microbe conglomerates in flea proventriculus and midgut was analysed and uniform mechanism of their formation was elucidated. The role of Yersinia pestis biofilm in plague agent preservation in the gut of ectoparasites and soil of rodents burrows was discussed. PCR analysis confirmed the presence of plague microbe in plague infected corpses and fleas excrements stored at +8-10 degreesC for 7 years 9 months.
Asunto(s)
Biopelículas/crecimiento & desarrollo , Reservorios de Enfermedades/microbiología , Siphonaptera/microbiología , Yersinia pestis/fisiología , Animales , Tracto Gastrointestinal/microbiología , Interacciones Huésped-Patógeno , Microscopía Electrónica , Siphonaptera/ultraestructuraRESUMEN
In vitro experiments have demonstrated that the lactoperoxidase system produces a bactericidal effect on salmonellae and shigellae. The physiological concentrations of the components of this system, making it possible to obtain a pronounced bactericidal effect, have been established. Lactobacilli have been shown to potentiate the effect of the lactoperoxidase system. The possibility of realizing the bactericidal properties of the lactoperoxidase system with respect to salmonellae and shigellae in the preparations of immune lactosera, intended for passive enteral immunization against intestinal infections, has been suggested.
Asunto(s)
Lactoperoxidasa/inmunología , Peroxidasas/inmunología , Salmonella/inmunología , Shigella sonnei/inmunología , Medios de Cultivo/metabolismo , Relación Dosis-Respuesta a Droga , Peróxido de Hidrógeno/metabolismo , Lactobacillus/enzimología , Lactobacillus/inmunología , Salmonella/crecimiento & desarrollo , Salmonella typhimurium/crecimiento & desarrollo , Salmonella typhimurium/inmunología , Shigella sonnei/crecimiento & desarrollo , Factores de TiempoRESUMEN
The phospholipid content of the old and new rabbit erythrocyte membrane has been studied. The new erythrocyte membranes were found to contain fewer sphingomyelins compared to both the total population and the old erythrocyte fraction. In the old erythrocyte membrane the amount of sphingomyelins increased, whereas that of phosphatidilcholins decreased. The contents of phosphatidiletanolamins and phosphatidilserins in the old and new erythrocytes did not change.