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The purpose of this work was to evaluate the use of an alternative transport medium supplemented with a cyanobacterial extract (CE), free of animal derivatives, to preserve the viability of Helicobacter pylori strains during long-term transportation and allow its recovery from biopsy samples. The transport media evaluated were Mueller-Hinton broth 0.3% agar (MH) and 0.3% of CE (MH-CE). MH broth 5% fetal calf serum (FCS) was used as the reference medium (MH-FCS). Biopsy samples from 134 patients, H. pylori NCTC 11638 and six clinical isolates were studied. A higher recovery (p ≤ 0.001) at 4°C was obtained in MH-CE than in MH-FCS after 96 h of storage. Only MH-CE allowed recovery after 120 h. The H. pylori recovery at room temperature after 96 h was higher (p ≤ 0.005) in MH-CE than in MH-FCS. Similar survival rates were observed in biopsy samples conserved in MH-CE and MH-FCS at 4°C. The recovery after 48 h at room temperature in MH-CE was higher (p ≤ 0.05) than MH-FCS and was the only medium allowing recovery after 72 h. The MH-CE medium is a simple, inexpensive and animal derivatives-free transport medium that can be used to preserve H. pylori viability and its recovery from biopsy samples.

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ETHNOPHARMACOLOGICAL RELEVANCE: The increasing resistance of Helicobacter pylori to antibiotics demands the search for novel compounds from plant based sources. Artemisia douglasiana Besser is widely used in Cuyo region (Argentina) as folk medicine for the treatment of gastric ailments. AIM OF STUDY: Based on our previous studies that Artemisia douglasiana exert cytoprotective actions against ethanol-induced gastric mucosal injury we assayed the anti-Helicobacter pylori effect of the Artemisia douglasiana extract and its active compound, dehydroleucodine. MATERIALS AND METHODS: The in vitro anti-bacterial activity of Artemisia douglasiana extract and its active compound, dehydroleucodine were determined against one standard strain and six clinical isolates of Helicobacter pylori by using the agar dilution methods. RESULTS: The results showed that both dehydroleucodine and Artemisia douglasiana extract had activity against the microorganism with MICs between 1-8 and 60-120 mg/L, respectively. CONCLUSIONS: Artemisia douglasiana may be a useful alternative treatment strategy principally in eradication of metronidazole and clarithromycin-resistant strain.

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The vacA and cagA genotypes of Helicobacter pylori exhibited distinct geographic distribution and correlation with severity of disease. In the above genotypes (obtained from 150 H. pylori-positive patients--139 with gastritis, 10 with ulcer and 1 patient with gastric cancer) combinations vacA s1/m1 and s2/m2 were detected using PCR in 75 and 25% of isolates, respectively, in patients with chronic gastritis. The of s1/m1 and s2/m2 combinations were also detected from ulcers (60 and 40%, respectively). The cagA was detected in 30% of isolates. Concentrated culture supernatants of 7 (64%) out of 11 H. pylori strains induced vacuolization in Vero cells in titers ranging from 1:5 to 1:40. The vacA s1 genotype was significantly associated with, but not predictive of the presence of vacuolating cytotoxin activity and the cagA gene.

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We studied here the effect of aqueous extracts of Larrea divaricata Cav on the growth of Helicobacter pylori. Results show that cold extract, infusion, decoction and simulated digestion had inhibitory activity at 0.04-0.1 mg/l against clarithromycin and metronidazole susceptible and resistant H. pylori strains. These results support the popular use of L. divaricata Cav in gastric disturbances and prompt further research to characterize these compounds with a therapeutic potential against gastric ulcers and gastric cancer associated with H. pylori.

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In this study we present a relationship between different gastroduodenal pathologies and Helicobacter pylori infection. We used four diagnosis invasive methods for H. pylori infection: urease test (UT), histopathology (H), Gram stain (G) and culture (C). The upper gastrointestinal endoscopy of 300 dyspeptic patients showed that 71.6% had erosive congestive gastropathies, 13.6% had duodenopathies, 5.6% had gastric ulcer, 6.3% had duodenal ulcer and 2.6% had probable gastric neoplasia. We also correlated the data of water intake source with the pathologies. The percentage of infected patients with H. pylori was determined using: a) two simultaneous reference tests (UT and H), 54.3%, b) each test UT = 55.0%, H = 59.0%, G = 51.3%, and C = 43.0%. Sex, age and the source of water ingested did not show statistically significant differences.

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In this study we present a relationship between different gastroduodenal pathologies and Helicobacter pylori infection. We used four diagnosis invasive methods for H. pylori infection: urease test (UT), histopathology (H), Gram stain (G) and culture (C). The upper gastrointestinal endoscopy of 300 dyspeptic patients showed that 71.6 had erosive congestive gastropathies, 13.6 had duodenopathies, 5.6 had gastric ulcer, 6.3 had duodenal ulcer and 2.6 had probable gastric neoplasia. We also correlated the data of water intake source with the pathologies. The percentage of infected patients with H. pylori was determined using: a) two simultaneous reference tests (UT and H), 54.3, b) each test UT = 55.0, H = 59.0, G = 51.3, and C = 43.0. Sex, age and the source of water ingested did not show statistically significant differences.(AU)

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In this study we present a relationship between different gastroduodenal pathologies and Helicobacter pylori infection. We used four diagnosis invasive methods for H. pylori infection: urease test (UT), histopathology (H), Gram stain (G) and culture (C). The upper gastrointestinal endoscopy of 300 dyspeptic patients showed that 71.6 had erosive congestive gastropathies, 13.6 had duodenopathies, 5.6 had gastric ulcer, 6.3 had duodenal ulcer and 2.6 had probable gastric neoplasia. We also correlated the data of water intake source with the pathologies. The percentage of infected patients with H. pylori was determined using: a) two simultaneous reference tests (UT and H), 54.3, b) each test UT = 55.0, H = 59.0, G = 51.3, and C = 43.0. Sex, age and the source of water ingested did not show statistically significant differences.

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RESUMEN

In this study we present a relationship between different gastroduodenal pathologies and Helicobacter pylori infection. We used four diagnosis invasive methods for H. pylori infection: urease test (UT), histopathology (H), Gram stain (G) and culture (C). The upper gastrointestinal endoscopy of 300 dyspeptic patients showed that 71.6

had erosive congestive gastropathies, 13.6

had duodenopathies, 5.6

had gastric ulcer, 6.3

had duodenal ulcer and 2.6

had probable gastric neoplasia. We also correlated the data of water intake source with the pathologies. The percentage of infected patients with H. pylori was determined using: a) two simultaneous reference tests (UT and H), 54.3

, b) each test UT = 55.0

, H = 59.0

, G = 51.3

, and C = 43.0

. Sex, age and the source of water ingested did not show statistically significant differences.

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It has been suggested that oral dissemination might be the major transmission vehicle for Helicobacter pylori, and that dental plaque might act as its reservoir. The presence of H. pylori was investigated in 62 odontological male and female patients (average age: 35 years old). Samples were taken from supragingival plaque, placed in 0.3 ml of thioglycolate broth, cultured within 12 h in Mueller-Hinton agar with the addition of 5-7% of sheep blood and antibiotic supplement, and incubated at 37 degrees C in microaerophilia for 5-7 days. Typical colonies were identified by gram, urease, oxidase and catalase. H. pylori was detected in a 15 year-old patient suffering from gastric acidity (1.61% positivity index). The medium used facilitated recovery of the agent from a sample abundant in germs. H. pylori was not recovered from the same patient 12 months later, suggesting that there might have been a transitory passage by gastric reflux or that the bacterium was acquired from an exogenous source.

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Se ha sugerido que la diseminación oral sería la principal vía de transmisión de Helicobacter pylori y la placa dental podría actuar como reservorio. Se investigó su presencia en 62 pacientes odontológicos de ambos sexos, edad promedio 35 años. Las muestras, tomadas de placas supragingival, colocadas en 0,3 ml de caldo tioglicolato, fueron cultivadas dentro de las 12 h en agar Mueller-Hinton con 5-7 por ciento de sangre de oveja y suplemento antibiótico e incubadas a 37ºC en microaerofilia 5-7 días. Las colonias típicas se identificaron por gram, ureasa, oxidasa y catalasa. H. pylori fue detectado en un paciente de 15 años que padecía acidez gástrica (índice de positividad 1,61 por ciento). El medio utilizado facilitó la

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Se ha sugerido que la diseminación oral sería la principal vía de transmisión de Helicobacter pylori y la placa dental podría actuar como reservorio. Se investigó su presencia en 62 pacientes odontológicos de ambos sexos, edad promedio 35 años. Las muestras, tomadas de placas supragingival, colocadas en 0,3 ml de caldo tioglicolato, fueron cultivadas dentro de las 12 h en agar Mueller-Hinton con 5-7 por ciento de sangre de oveja y suplemento antibiótico e incubadas a 37§C en microaerofilia 5-7 días. Las colonias típicas se identificaron por gram, ureasa, oxidasa y catalasa. H. pylori fue detectado en un paciente de 15 años que padecía acidez gástrica (índice de positividad 1,61 por ciento). El medio utilizado facilitó la recuperación del agente desde una muestra abundante en gérmenes. No se aisló H. pylori del mismo paciente 12 meses después, sugiriendo un pasaje transitorio por reflujo gástrico o adquisición de la bacteria de una fuente exógena

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Se investigó la presencia de Helicobacter pylori en 50 pacientes con trastornos gastroduodenales que concurrieron a dos centros de salud de la ciudad de San Luis. De cada paciente se tomaron cuatro muestras de biopsia de mucosa de antro gástrico, dos de ellas destinadas al estudio histológico y dos al análisis bacteriológico: observación al Gram, prueba de ureasa y cultivo. Helicobacter pylori se detectó en 38 (76 por ciento) de los pacientes mediante el estudio histológico y en 30 (60 por ciento) por la tinción de Gram. De estos últimos, 28 (93 por ciento) dieron positiva la prueba de ureasa coincidiendo con un número significativo de bacterias. El 80 por ciento (24/30) de las muestras positivas al Gram mostró un buen desarrollo microbiano en los medios de cultivo de Mueller-Hinton y de Skirrow indistintamente. Se recomienda la prueba de ureasa como una alternativa de diagnóstico: rápida, económica y efectiva cuando hay una cantidad suficiente de bacterias. (AU)

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Se investigó la presencia de Helicobacter pylori en 50 pacientes con trastornos gastroduodenales que concurrieron a dos centros de salud de la ciudad de San Luis. De cada paciente se tomaron cuatro muestras de biopsia de mucosa de antro gástrico, dos de ellas destinadas al estudio histológico y dos al análisis bacteriológico: observación al Gram, prueba de ureasa y cultivo. Helicobacter pylori se detectó en 38 (76 por ciento) de los pacientes mediante el estudio histológico y en 30 (60 por ciento) por la tinción de Gram. De estos últimos, 28 (93 por ciento) dieron positiva la prueba de ureasa coincidiendo con un número significativo de bacterias. El 80 por ciento (24/30) de las muestras positivas al Gram mostró un buen desarrollo microbiano en los medios de cultivo de Mueller-Hinton y de Skirrow indistintamente. Se recomienda la prueba de ureasa como una alternativa de diagnóstico: rápida, económica y efectiva cuando hay una cantidad suficiente de bacterias.

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