RESUMEN
Campylobacter is considered the most common bacterial cause of human gastroenteritis in the world with C. jejuni being regarded as the primary cause of bacterial gastroenteritis. A broad range of other Campylobacter species, including C. coli have also been implicated in human gastroenteritis. This study sought to isolate, characterize and assess the antibiogram of Campylobacter jejuni and C. coli from faecal samples obtained from cats and dogs in Isfahan and Shahrekord cities in Iran. Faecal samples were collected from 100 pets comprising of 50 dogs and 50 cats from March 2015 to March 2016; incorporating the four seasons (spring, summer, autumn and winter). Campylobacter spp. was isolated by culture, characterized by biochemical tests and confirmed by PCR-based assays. Antimicrobial susceptibility test was performed by the Kirby-Bauer disk diffusion method, using Mueller Hinton agar. A total of 19 Campylobacter isolates among them two C. jejuni and one C. coli were recovered from dogs and cats' faecal samples. The prevalence rates of Campylobacter spp. were 16.0% (8 out of 50) in dogs and 22.0% (11 out of 50) in cats. The highest (4 out of 16, 25%) Campylobacter spp. prevalence among dogs was reported in autumn and the lowest (1 out of 11, 9.1%) in spring, while among the cats, the highest (4 out of 12, 33.3%) Campylobacter spp. prevalence was reported in summer and lowest (1 out of 11, 9.09%) in spring. Campylobacter spp. isolated from faecal samples obtained from cats and dogs exhibited the most frequent antimicrobial resistance against tetracycline at 81.8% and 87.5%, respectively, compared to all other antimicrobial agents. These results show a low prevalence of Campylobacter spp. in faecal samples obtained from pet dogs and cats in Shahrekord and Isfahan cities in Iran. Given the relatively low prevalence of the C. jejuni and C. coli in pet dogs and cats in Isfahan and Shahrekord cities, it can be assumed that their importance as reservoirs for infection in humans is likely to be limited to the studied cities, but should not be neglected.
Asunto(s)
Infecciones por Campylobacter/veterinaria , Campylobacter/clasificación , Enfermedades de los Gatos/microbiología , Enfermedades de los Perros/microbiología , Animales , Antibacterianos/farmacología , Campylobacter/aislamiento & purificación , Infecciones por Campylobacter/epidemiología , Enfermedades de los Gatos/epidemiología , Gatos , Enfermedades de los Perros/epidemiología , Perros , Farmacorresistencia Bacteriana , Heces/microbiología , Calor , Irán/epidemiología , PrevalenciaRESUMEN
This study was undertaken to investigate molecularly the occurrence of EHV-1 and EHV-4 infection among equine population in regions, Iran. Blood samples from 53 and 37 randomly selected horses settled in Isfahan and Shahrekord, Iran, respectively, were collected. Detection of EHV-1 and EHV-4 genes in the blood samples was done using polymerase chain reaction (PCR). Out of 53 and 37 samples from Isfahan and Shahrekord, 4 (18.18%) and 3 (8.10%) were positive for PCR of EHV-1, respectively. Nine (16.98%) and 6 (16.21%) were positive for PCR of EHV-4, while 6 (11.32%) and 3 (8.10%) were positive for PCR of both EHV-1 and EHV-4, in Isfahan and Shahrekord, respectively. Of the 7 blood samples positive for EHV-1, 4 (16.66%) and 3 (8.10%) were from horses >3 years old while 2 (18.18%) and 1 (16.66%) were from 2-3 years old horses, in Isfahan and Shahrekord, respectively. Out of the 7 and 3 samples positive for PCR of EHV-1 in Isfahan and Shahrekord, 4 (22.2%) and 1 (7.69%) were Standardbred, while 3 (14.28%) and 2 (13.33%) were Thoroughbreds, respectively. EHV-4 was detected in blood of 4 (22.22%) and 2 (15.83%) Standardbreds and from 4 (19.04%) and 4 (26.66%) Thoroughbred horses in Isfahan and Shahrekord, respectively. This study has shown that horses settled in Isfahan central and Shahrekord southwest regions, Iran, are infected by EHV-1 and EHV-4 and thus serve as potential reservoirs and disseminators of the viruses.