Asunto(s)
Antígenos Virales/análisis , Virus de la Influenza A/inmunología , Virus de la Influenza B/inmunología , Manejo de Especímenes/métodos , Adulto , Anticuerpos Monoclonales , Anticuerpos Antivirales/análisis , Ensayo de Inmunoadsorción Enzimática , Técnica del Anticuerpo Fluorescente , Humanos , Inmunoquímica , Virus de la Influenza A/aislamiento & purificación , Virus de la Influenza B/aislamiento & purificación , Metales de Tierras Raras , Nasofaringe/microbiologíaRESUMEN
The comparative study of the diagnostic value of the enzyme immunoassay (EIA), indirect immunofluorescence (IF) and countercurrent immunoelectrophoresis (CIE) was made. The serological identification of the isolated and reference pneumococci (19) and H. influenzae (38) strains revealed the possibility of using all three microanalytical methods for this purpose. The study of pneumococcal and H. influenzae antigens in native sputum obtained from 74 patients with acute pneumonia showed that EIA and indirect IF were highly sensitive, their sensitivity considerably exceeding that of the bacteriological analysis. Pneumococcal antigens were detected in 66.2% of patients by EIA and in 54.0% of patients by indirect IF, while H. influenzae antigens were detected in 58.1% of patients by EIA and in 67.6% of patients by indirect IF. The sensitivity of CIE proved to be considerably lower; in the detection of pneumococcal antigens it was level with the sensitivity of the bacteriological analysis (23.0%) and H. influenzae antigens could be detected only in 27.0% of patients.
Asunto(s)
Anticuerpos Antibacterianos/análisis , Antígenos Bacterianos/análisis , Infecciones por Haemophilus/diagnóstico , Haemophilus influenzae/inmunología , Neumonía Neumocócica/diagnóstico , Neumonía/diagnóstico , Streptococcus pneumoniae/inmunología , Enfermedad Aguda , Técnica del Anticuerpo Fluorescente , Infecciones por Haemophilus/microbiología , Haemophilus influenzae/clasificación , Haemophilus influenzae/aislamiento & purificación , Humanos , Inmunoelectroforesis , Técnicas para Inmunoenzimas , Neumonía/microbiología , Neumonía Neumocócica/microbiología , Serotipificación , Esputo/microbiología , Streptococcus pneumoniae/clasificación , Streptococcus pneumoniae/aislamiento & purificaciónRESUMEN
The etiology of the disease, the age structure of fatalities, the time of death were studied in 34 fatal cases in the period of influenza A (H1N1) epidemic in December 1977-February 1978 and in 33 fatal cases during influenza A/Victoria/3/75 (H3N2) epidemic in January-March, 1975 (data from some autopsy offices of Kiev). The results of the investigation of the etiology by immunofluorescence and the histological methods were mostly similar. The polyetiological structure of the diseases in the influenza epidemic of 1975 and rather monoetiological one in the influenza epidemic of 1977-1978 were observed. In 1975, fatal cases were found mostly in 2 age groups: infants under 1 year and adults over 20, in 1977-1978 in 4 age groups: infants under 1, children of 1-3, over 9, and adults. In both epidemics the maximum number of fatal cases coincided with the beginning of a decline in the epidemic wave and subsequently they were registered evenly for over one month. A high infection rate with a new influenza A virus variant (H1N1) was found among normal subjects in all age groups.
Asunto(s)
Brotes de Enfermedades/epidemiología , Gripe Humana/etiología , Adolescente , Adulto , Antígenos Virales/análisis , Preescolar , Femenino , Humanos , Lactante , Virus de la Influenza A/inmunología , Gripe Humana/inmunología , Gripe Humana/mortalidad , Gripe Humana/patología , Masculino , U.R.S.S.Asunto(s)
Técnica del Anticuerpo Fluorescente , Gripe Humana/diagnóstico , Meningitis/diagnóstico , Infecciones del Sistema Respiratorio/diagnóstico , Adolescente , Adulto , Anciano , Femenino , Humanos , Gripe Humana/inmunología , Masculino , Meningitis/etiología , Meningitis/inmunología , Persona de Mediana Edad , Enfermedades Respiratorias/inmunologíaRESUMEN
Infection with adeno-associated viruses (AAV) early in life and extensive dissemination of these viruses in infants were discovered by detection of AAV antigen by the fluorescent antibody procedure in autopsy materials from infants dying of acute respiratory viral diseases. AAV antigens were found in cells from various organs of infants aged 2,5, 7, 9 days and older. In each individual case AAV of the same serological type was found in different organs. In 4-months-old twins AAV antigens of the same serotypes, 1 and 4, were found in the trachea, lungs, liver, kidney, brains. Out of 21 infants dying of adenovirus infection, 20 had AAV antigens the distribution of which in cells of various organs was analogous to that of the adenovirus antigen, with a few exceptions. Three infants had no adenovirus infection and no AAV antigne. In the other 6 infants no adenovirus antigen but AAV antigens were found. In the latter cases herpes virus infection is not excluded. Possible modes of transmission of AAV infection are discussed.