RESUMEN

The phytohemagglutinin effect in vivo on monooxygenase activity depends on the type of the cells under study (thymocytes, splenocytes, macrophages, hepatocytes) and on the period after lectin injection. Variations of monooxygenase activities associated with different forms of cytochrome P-450 are established to have a number of peculiarities in isolated hepatocytes as compared to those in the liver microsome fraction. These differences are supposed to be due to the effect of nonmicrosomal cytochrome P-450. The results obtained are discussed with regard for phytohemagglutinin effect on systems regulating activity of cytochrome P-450-dependent monooxygenases.

Asunto(s)

Hígado/enzimología , Linfocitos/enzimología , Macrófagos/enzimología , Oxigenasas/metabolismo , Fitohemaglutininas/farmacología , Animales , Benzopireno Hidroxilasa/metabolismo , Membrana Celular/enzimología , Membrana Celular/metabolismo , Sistema Enzimático del Citocromo P-450 , Hígado/citología , Hígado/metabolismo , Linfocitos/metabolismo , Macrófagos/metabolismo , Masculino , Ratones , Ratones Endogámicos CBA