RESUMEN
BACKGROUND: Oral squamous cell carcinoma (OSCC) is one of the most common cancers in the head and neck squamous cell cancer group. The increasing frequency of oral carcinomas and their late-stage appearance is a major worldwide health concern. MicroRNAs (miRNAs) appear to play an important role in cancer growth and progression, according to growing data, whereas no information is available regarding miR-7113-3p and miR-6721-5p involvement in OSCC. In this article, the expression of MAP2K1, miR-7113-3p, and miR-6721-5p was examined for possible bio-logical functions in the advancement of oral squamous cell carcinoma. MATERIAL AND METHODS: We used quantitative real-time PCR (to examine the mRNA expression of MAP2K1, miR-7113-3p, and miR-6721-5p in fresh frozen OSCC tissues and adjacent normal fresh frozen tissues from 30 patients, and we investigated their relationship with clinical parameters. RESULTS: MAP2K1 expression was found to be dramatically increased in tumor tissues than in normal tissues, whereas miR7113-3p and miR-6721-5p expression was significantly decreased. Furthermore, a statistical correlation of P = 0.04 was also observed between increased MAP2K1 expression and perineural invasion. Additionally, we noted that the downregulation of miR-7113-3p appears to correlate positively with overexpression of MAP2K1 (P = 0.0218), and a negative correlation was observed between downregulation of miR-6721-5p and overexpression of MAP2K1 (P = 0.7771). CONCLUSION: Based on these findings, miR-7113-3p and miR-6721-5p might be prospective bio-markers for OSCC patients, and could be utilized to detect OSCC at an early stage for future dia-gnosis. MAP2K1 overexpression has been linked to the development of OSCC and perineural invasion.
Asunto(s)
Regulación Neoplásica de la Expresión Génica , MicroARNs , Neoplasias de la Boca , Microambiente Tumoral , Humanos , Neoplasias de la Boca/genética , Neoplasias de la Boca/patología , Neoplasias de la Boca/metabolismo , MicroARNs/genética , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patología , Carcinoma de Células Escamosas/metabolismo , MAP Quinasa Quinasa 1/genética , MAP Quinasa Quinasa 1/metabolismo , Carcinoma de Células Escamosas de Cabeza y Cuello/genética , Carcinoma de Células Escamosas de Cabeza y Cuello/patología , Carcinoma de Células Escamosas de Cabeza y Cuello/metabolismoRESUMEN
AIM: The purpose of this study was to determine the prognostic importance of positive exercise treadmill test (ETT) in predicting coronary artery disease (CAD) among asymptomatic population based on coronary angiographic findings. METHODS: The comparative study was conducted on a sample of over 60-year-old asymptomatic and symptomatic patients referred to our clinics for screening and clinical reasons between September 2006 and September 2007. All subjects with a positive ETT provided information about their cardiovascular risk factors through a validated questionnaire. The relationship between ETT performance and the CAD risk factors with coronary angiography were studied and compared between the two groups. RESULTS: Seven hundred thirty one symptomatic and 160 asymptomatic individuals were enrolled in the study. In the symptomatic group, gender, blood pressure and diabetes were significantly related with the angiographic findings, while blood pressure failed to show such a relation in the asymptomatic group. CONCLUSION: There is an association between positive ETT and the presence of CAD among asymptomatic elderly men but also women with diabetes or high blood pressure.