RESUMEN
A new series of new 1,4-naphthoquinone derivatives containing carbazole-6,11-dione moiety, which has not been reported yet, has been synthesized from 1,4-naphthoquinone and 4-aminophenylsulfone involving a Michael addition, benzoylation, and Pd-catalyzed coupling. This set of compounds has been evaluated for in vitro antibacterial studies against different Gram-positive and Gram-negative bacteria, and most of the synthesized compounds exhibited good antibacterial activity and the minimum inhibitory concentrations (MICs) are compared with the standard drugs used. Compound 7 exhibited good antibacterial activity among all the molecules studied with the best MIC of 2.1 µg/mL against Bacillus subtilis. To understand the molecular interactions with targeted proteins, the molecular docking of all the synthesized compounds were carried out; between 14 molecules docked, compound 7 was the one with the best glide and E model score of -7.73 and -95.37, respectively. In all docked molecules, compound 5 exhibited least glide and E model score of -4.55 and -101.56, respectively. Figureá
RESUMEN
A new series of 2-(4-aminobenzosulfonyl)-5H-benzo[b]carbazole-6,11-dione derivatives, which has not been reported yet, has been synthesized from 1,4-naphthoquinone and 4-aminophenylsulfone involving an Michael addition, benzoylation and Pd catalyzed coupling. This set of compounds has been evaluated for in vitro cytotoxicity specifically against human cervical cancer cell line (SiHa) and most of the synthesized compounds exhibited good cytotoxic activity. Molecular docking of all the synthesized compounds was studied; among fourteen molecules docked compound 3 was the one with the best glide and E model score of -9.06 and -73.41, respectively which is close to the glide score of SAHA (standard). In all docked molecules, the compound 7a exhibits least glide and E model score of -2.97 and -71.02 respectively.
Asunto(s)
Carbazoles/química , Inhibidores de Histona Desacetilasas/síntesis química , Proteínas Represoras/antagonistas & inhibidores , Sulfonas/química , Sitios de Unión , Carbazoles/síntesis química , Carbazoles/toxicidad , Dominio Catalítico , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Inhibidores de Histona Desacetilasas/química , Inhibidores de Histona Desacetilasas/toxicidad , Histona Desacetilasas/metabolismo , Humanos , Simulación del Acoplamiento Molecular , Naftoquinonas/química , Proteínas Represoras/metabolismoRESUMEN
The kinetics of oxidation of eight structurally different amino acids by hypobromite ion (BrO(-)) in the presence of hydroxide ion has been studied. The reactions proceed through the rapid formation of N-bromoamino acid anion which then decomposes in the rate-limiting step. The decomposition of N-bromoamino acid anions is found to proceed through an unimolecular and a base-catalyzed reaction. The large negative values of DeltaS() and the products formed suggest that the hydroxide ion-catalyzed reactions proceed through an E(2) mechanism. N-Bromoamino acid anion gives an absorption spectrum with lambda(max) at approximately 290 nm.