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Introduction: This in-vitro study comprises Antimicrobial and Confocal Microscopic analysis of the efficacy of Triple Antibiotic and Toluidine Blue (TBO) loaded chitosan nanoparticles (chnp) activated with photodynamic therapy (PDT) against Enterococcus faecalis (Ef). Methodology: 105 single-rooted extracted teeth were decoronated, cleaned and shaped, and incubated with Ef. The roots were randomly divided into three groups: Group I-Control, Group II-Triple Antibiotic Paste (TAP), Group III: Triple antibiotic-loaded chitosan nanoparticles (tachnp), and Group IV: Tachnp and TBO loaded chnp activated with PDT. Ten specimens from all the groups were subjected to antimicrobial analysis and five specimens were observed under the confocal microscope after 24 h and 7 days. Group IV was additionally analyzed after 24 h and 7 days of laser activation. Results: Group IV C showed the least colony-forming units followed by Group IVB, Group IIIB, Group IVA, and Group IIIA. Group II showed more colony-forming units. On intergroup comparison of mean colony count in control and experimental groups at 24 h and 7 days using one-way ANOVA F test was highly significant P < 0.001. The confocal microscopic images of IV C showed the increased intensity of fluorescent red indicating dead bacteria. Conclusion: Within the limitations of this study, TAP loaded chitosan nanoparticles activated with PDT showed a significant reduction in colony count.
RESUMEN
AIM: To evaluate the effect of different irrigating solutions on postspace treatments on the push-out bond strength of glass fiber posts. MATERIALS AND METHODS: Thirty mandibular premolar roots were decoronated and endodontically treated. Postspaces were prepared and roots were divided into three groups: In group 1: 2.5% sodium hypochlorite irrigation (control), group 2: 17% ethylenediaminetetraacetic acid (EDTA) with hand activation, group 3: 17% EDTA irrigation with photon-induced photoacoustic streaming (PIPS) has been done to the postspaces. Scanning electron microscope (SEM) analysis has been made for two samples of each group. Fiber posts were then luted with resin cement. Each root was prepared for push-out test. Data have been statistically analyzed. RESULTS: SEM results showed clean postwalls with both group 2 and group 3, whereas group 1 showed adhesion of resin cement to intraradicular dentine. When all groups were compared, the bond strength values are higher with group 2 followed by group 3. CONCLUSION: Within the limitations of the study, clean postwalls and the highest bond strength values were obtained from 17% EDTA with hand activation and 17% EDTA with PIPS.