RESUMEN
Despite the ubiquitous and persistent presence of microplastic (MP) in marine ecosystems, knowledge of its potential harmful ecological effects is low. In this work, we assessed the risk of floating MP (1 µm-5 mm) to marine ecosystems by comparing ambient concentrations in the global ocean with available ecotoxicity data. The integration of twenty-three species-specific effect threshold concentration data in a species sensitivity distribution yielded a median unacceptable level of 1.21 ∗ 105 MP m-³ (95% CI: 7.99 ∗ 103-1.49 ∗ 106 MP m-³). We found that in 2010 for 0.17% of the surface layer (0-5 m) of the global ocean a threatening risk would occur. By 2050 and 2100, this fraction increases to 0.52% and 1.62%, respectively, according to the worst-case predicted future plastic discharge into the ocean. Our results reveal a spatial and multidecadal variability of MP-related risk at the global ocean surface. For example, we have identified the Mediterranean Sea and the Yellow Sea as hotspots of marine microplastic risks already now and even more pronounced in future decades.
Asunto(s)
Plásticos , Contaminantes Químicos del Agua , Ecosistema , Monitoreo del Ambiente , Mar Mediterráneo , Microplásticos , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidadRESUMEN
Global change will disturb the frequency, scale and distribution of harmful algal blooms (HABs), but we are unable to predict future HABs due to our limited understanding of how physicochemical changes in the environment affect interspecific competition between dinoflagellates. Trait-based mechanistic modelling is an important tool to unravel and quantify various direct and indirect interactions between species. The present study explores whether MacArthur's consumer-resource model can be used as a viable base model to predict dinoflagellate growth in closed multispecies systems. To this end, two batch culture experiments (294 cultures in total) with monocultures and multispecies cultures of Alexandrium minutum, Prorocentrum lima, P. micans, Protoceratium reticulatum and Scrippsiella trochoidea were performed. Despite changes to the relative (different nitrate concentrations) and absolute nutrient availability (dilutions of L1 medium), P. micans outcompeted all other species in mixed cultures. Consumer-resource modelling parameterized using monoculture growth correctly predicted this species dominance (R² between 0.80 and 0.95). Parameter estimates revealed that P. micans had a faster uptake of nitrogen when compared to its competitors, but did not differ in resource efficiency and natural mortality rate. Yet, while the model accurately predicted community dynamics during the growth phase, it was not able to predict their dynamics beyond the point of quiescence. Consumer-resource modelling was shown to differentiate the roles of resource assimilation, resource efficiency, and natural mortality rates in batch culture experiments with minimal data requirements beyond common measurements. The results suggest that consumer-resource models provide a promising basis for trait-based modelling of interspecific competition between (harmful) algae.
Asunto(s)
Dinoflagelados , Técnicas de Cultivo Celular por Lotes , Floraciones de Algas Nocivas , Nitratos , NitrógenoRESUMEN
Like marine diseases, harmful algal blooms (HABs) are globally increasing in frequency, severity and geographical scale. As a result, bivalves will have to face the combined threat of toxic algae and marine pathogens more frequently in the (near) future. These stressors combined may further affect the recruitment of ecologically and economically important bivalve species as HABs can affect the growth, viability and development of their larvae. To date, little is known on the specific effects of HABs on the innate immune system of bivalve larvae. This study therefore investigates whether two common harmful algae can influence the larval viability, development and immunological resilience of the blue mussel Mytilus edulis. Embryos of this model organism were exposed (48 h) to five densities of Pseudo-nitzschia multiseries or Prorocentrum lima cells. In addition, the effect of six concentrations of their respective toxins: domoic acid (DA) and okadaic acid (OA) were assessed. OA was found to significantly reduce larval protein phosphatase activity (p < 0.001) and larval viability (p < 0.01) at concentrations as low as 37.8 µg l(-1). P. multiseries (1400 cells ml(-1)), P. lima (150 cells ml(-1)) and DA (dosed five times higher than typical environmental conditions i.e. 623.2 µg l(-1)) increased the phenoloxidase (PO) innate immune activity of the mussel larvae. These results suggest that the innate immune response of even the earliest life stages of bivalves is susceptible to the presence of HABs.
Asunto(s)
Floraciones de Algas Nocivas , Inmunidad Innata/efectos de los fármacos , Ácido Kaínico/análogos & derivados , Toxinas Marinas/toxicidad , Mytilus edulis/efectos de los fármacos , Mytilus edulis/inmunología , Ácido Ocadaico/toxicidad , Animales , Diatomeas/química , Dinoflagelados/química , Desarrollo Embrionario/efectos de los fármacos , Ácido Kaínico/toxicidad , Larva/efectos de los fármacos , Larva/enzimología , Larva/crecimiento & desarrollo , Larva/inmunología , Mytilus edulis/enzimología , Mytilus edulis/crecimiento & desarrollo , Fosfoproteínas Fosfatasas/genética , Fosfoproteínas Fosfatasas/metabolismoRESUMEN
A range of environmental factors, including chemicals, can affect epigenetic processes in organisms leading to variations in phenotype. Thus, epigenetics displays an important environmentally responsive element. The transgenerational impact of environmental stressors on DNA methylation and phenotype was the focus of this study. The influence of two known DNA methylation-changing agents, the phytoestrogen genistein and the fungicide vinclozolin, on the overall DNA methylation level in the Asian tiger mosquito Aedes albopictus was investigated. The experiment comprised four generations in a full life-cycle design with an exposed parental generation and three consecutive non-exposed offspring generations. Application of the methylation agents to the parental generation of the study led to an alteration of the global DNA methylation level of the exposed individuals and those in two subsequent generations. The phenotypic variability of the offspring generations was assessed by examining their insecticide sensitivity. Here, a significant decrease in sensitivity (p<0.01) towards the model insecticide imidacloprid revealed alterations of the mosquito's phenotype in two subsequent generations. Thus, the evaluation of A. albopictus from an epigenetic perspective can contribute important information to the study of the high adaptability of this invasive disease vector to new environments, and its underlying mechanisms.
Asunto(s)
Aedes/efectos de los fármacos , Metilación de ADN/efectos de los fármacos , Epigénesis Genética/efectos de los fármacos , Imidazoles/farmacología , Insecticidas/farmacología , Nitrocompuestos/farmacología , Adaptación Fisiológica/efectos de los fármacos , Adaptación Fisiológica/genética , Aedes/genética , Aedes/crecimiento & desarrollo , Animales , Vectores de Enfermedades , Tolerancia a Medicamentos , Ecotoxicología , Genisteína/toxicidad , Humanos , Neonicotinoides , Oxazoles/toxicidad , ReproducciónRESUMEN
Little research on the epigenetic phenomenon of DNA methylation has been performed with invertebrates. However, a few studies have shown that effects of transient chemical exposure can be transferred to non-exposed generations through epigenetic inheritance. If this is a wide-spread phenomenon it may have major consequences for current ecological risk assessments. The presence of DNA methylation in Daphnia magna has only very recently been demonstrated, although not quantified. In the present study, the overall content of 5-methyl-2'-deoxycytidine (5 mdC) in the DNA of D. magna was determined. Additionally, the effect of transgenerational Zn exposure on the 5 mdC content was assessed. An optimized LC-MS-MS method proved to be very suitable for measuring overall levels of 5 mdC in relatively small samples of D. magna DNA. The average [5 mdC]/[dG] ratio ranged from 0.13% to 0.81%. A slight but significant decrease in DNA methylation was found in the offspring (F1) of Zn exposed daphnids in the parental generation. However, this effect did not propagate into the next generation (F2). The presence of DNA methylation in D. magna and the finding that different exposure histories can entail different levels of methylation, open the way for further investigations on the implications of epigenetics in aquatic toxicology.
Asunto(s)
Metilación de ADN , Daphnia/genética , Zinc/toxicidad , Animales , Cromatografía Liquida/métodos , ADN/química , Daphnia/efectos de los fármacos , Desoxicitidina/análogos & derivados , Desoxicitidina/análisis , Espectrometría de Masas en Tándem/métodosRESUMEN
It is generally accepted that the bioavailability of metals in sediments is influenced by the presence of acid volatile sulfides (AVS). The pore water hypothesis predicts that, if the molar concentration of simultaneously extracted metals (SEM) in a sediment is smaller than the molar concentration of AVS, the free metal ion activity in the pore water is very small and that consequently no metal toxicity in short-term toxicity tests is observed. In this study we examined (1) if this concept can be extended to predict the absence of chronic Ni toxicity to the oligochaete deposit-feeding worm Lumbriculus variegatus and (2) if the organic carbon normalized excess SEM; i.e. [SEM-AVS]/f(OC) predicts the magnitude of Ni toxicity to L. variegatus. A 28-day toxicity experiment was performed in which biomass production of L. variegatus was determined in two natural sediments with different [AVS] and f(OC), spiked at different Ni concentrations. The absence of toxicity is predicted correctly by the [SEM-AVS]<0 criterion when only the 0-1 cm surface layer of the sediment is considered, but not when the whole bulk sediment is considered (0-3 cm). In both sediments, the same [SEM-AVS]/f(OC) at the surface corresponds with a similar decrease in L. variegatus biomass. Thus, [SEM-AVS]/f(OC) in the surface layer accurately predicts the magnitude of toxicity. This measure is therefore a good estimator of toxicologically available Ni. On the other hand, the free Ni(2+) ion activity in the overlying water appeared to be an equally good predictor of the magnitude of toxicity. Consequently, it was not possible to determine the relative importance of the overlying water and pore water exposure route with the semi-static laboratory experiments.