RESUMEN

IFN-γ is the most commonly measured cytokine released by the cells to define the cellular immune responses induced by the vaccine candidates for tuberculosis. IL-15 acts as a co-stimulator in IFN-γ production by NK cells and may therefore be important in the control of Mycobacterium tuberculosis that requires IFN-γ for clearance. The aim of the study is to determine whether Ag85A can also stimulate the innate immune response through the expression of IL-15, a cytokine that bridges the innate and adaptive immune systems. The expression of IL-15 was up regulated by about 4 fold in PPD+ healthy controls as compared with TB patients. Significantly higher expression of IL-15 mRNA in the Ag85A stimulated cells not only in PPD+ healthy controls but also in TB patients substantiates the use of Ag85A as a vaccine candidate over ESAT-6.

Asunto(s)

Aciltransferasas/inmunología , Antígenos Bacterianos/inmunología , Interleucina-15/biosíntesis , Mycobacterium tuberculosis/inmunología , Vacunas contra la Tuberculosis/inmunología , Adolescente , Adulto , Estudios de Casos y Controles , Células Cultivadas , Femenino , Humanos , Inmunidad Innata , Interferón gamma/biosíntesis , Interleucina-15/genética , Células Asesinas Naturales/inmunología , Recuento de Linfocitos , Masculino , Persona de Mediana Edad , ARN Mensajero/genética , Tuberculosis Pulmonar/inmunología , Regulación hacia Arriba/inmunología , Adulto Joven