RESUMEN

We focused our study on the olfactory cells growth on biocompatible polymer films electrodeposited on a silicon microsystem. Several substrates such as polyethyleneimine (PEI), polypropyleneimine (PPI), and polypyrrole (PPy), acting as potentially good candidates for cell culture, were tested in order to allow cells to adhere and proliferate. During their growth, the evolution of their morphology was monitored using both confocal microscope and immunohistochemistry, leading to the conclusion of a normal development. An estimation of the adhesion and proliferation rates of rat neuronal cell cultures indicated that PEI and PPI were the best substrates for cultivating olfactory cells.

Asunto(s)

Técnicas Biosensibles/métodos , Técnicas de Cultivo de Célula/métodos , Materiales Biocompatibles Revestidos/química , Neuronas/citología , Neuronas/fisiología , Bulbo Olfatorio/fisiología , Polímeros/química , Animales , Técnicas Biosensibles/instrumentación , Adhesión Celular/fisiología , Técnicas de Cultivo de Célula/instrumentación , Línea Celular , Proliferación Celular , Ensayo de Materiales , Ratones , Bulbo Olfatorio/citología , Ratas