RESUMEN
This study aimed to screen 10 medicinal plant extracts on zebrafish (Danio rerio), evaluating their impact on the complement system, immunoglobulin M (IgM) levels, lysozyme, and peroxidase activity, while also enhancing their efficacy through the gradual release using alginate-chitosan nanocapsules. The prepared methanolic extracts were combined with fish feed. The fish were divided into 12 groups, including 10 treatment groups, a positive and a negative control group. Results showed varying impacts of the extracts on the immune and antioxidant systems, with Cinnamon (Cinnamon cassia) and Hypericum (Hypericum perforatum) extracts demonstrating the most significant effects. Subsequently, Cinnamon and Hypericum extract were encapsulated in alginate-chitosan nanocapsules to assess their impact on zebrafish immune parameters, separately and synergistically. Gradual release of the extracts from the nanocapsules was observed, with slower release at pH 2 compared to pH 7. Overall, Cinnamon and Hypericum extracts exhibited substantial immune system enhancement, and their encapsulation in nanocapsules improved their effects on zebrafish immune parameters. These findings suggest using these encapsulated extracts to enhance immune responses in aquatic organisms.
RESUMEN
Kisspeptin (kp) is a key regulator of reproduction, which stimulates sexual maturation and gametogenesis in mammals, amphibians, and teleosts. In the present study, to enhance the biological activity of kp10, a novel analog (referred to as M-kp10) was designed based on the endogenous goldfish variant, in which phenylalanine 6 was substituted by tryptophan and the N-terminus was acetylated. Compared with the native kp-10 and salmon gonadotropin-releasing hormone (GnRH3), the effect of M-kp10 on sexual hormones and reproductive indices as well as the expression of kiss1, cyp19a1, and kiss1ra genes in goldfish (Carassius auratus) was investigated. In practice, peptides were synthesized based on the standard Fmoc-solid-phase peptide synthesis and purified by employing RP-HPLC, followed by approving their structure using ESI-MS. The results showed that M-kp10 increased significantly 17,20ß-DHP, LH, FSH and E2 as well as fecundity, hatching and fertilization percentages than the other peptides. Histological studies revealed that M-kp10 led to the faster growth of ovarian follicles compared to the kp-10 and GnRH3. The genes of cyp19a1, kiss1ra, and kiss1 were remarkably more expressed after treatment with M-kp10. In conclusion, the results indicated the superiority of M-kp10 over kp-10 in inducing sexual maturation and accelerating the percentage of fecundity, suggesting that M-kp10 could be a promising candidate for application in the artificial breeding of fish.
Asunto(s)
Carpa Dorada , Kisspeptinas , Animales , Femenino , Kisspeptinas/metabolismo , Carpa Dorada/genética , Hormona Liberadora de Gonadotropina/metabolismo , Reproducción , Mamíferos/metabolismoRESUMEN
Kisspeptin, upstream of the hypothalamic-pituitary-gonadal axis, play an essential role in the reproductive process. In the present study, the effect of different types of kisspeptin, including goldfish (Carassius auratus) kiss1 kisspeptin (Kiss1), human kisspeptin (Hkiss) and their combination (Kiss1+H) on the reproductive-related genes (kiss1, Kissr and Cyp19) of adult female goldfish was investigated in comparison with Ovaprim (a synthetic GnRH hormone). Kiss1 and Hkiss were synthesized using a solid-phase synthesis approach. Peptides were injected at a dose of 100 µg/kg body weight. The brain and ovarian tissues of samples were separated for histological studies 24 hr post-injection. The expression of the kiss1, Kissr and Cyp19 genes was measured by RT-PCR. The results showed a significant increase in expression of the reproductive-related genes. Histological analysis revealed higher number of mature oocytes in kisspeptin treated groups compare to other ones. In conclusion, Hkiss and Kiss1+H are the most effective peptides in oocyte maturation and expression of reproductive-related genes. In addition, it seems that kisspeptins in other domestic animals can be used to stimulate the hypothalamus-pituitary-gonadal axis.
Asunto(s)
Carpa Dorada/fisiología , Kisspeptinas/farmacología , Oocitos/efectos de los fármacos , Animales , Aromatasa/genética , Encéfalo/metabolismo , Domperidona/farmacología , Combinación de Medicamentos , Femenino , Expresión Génica/efectos de los fármacos , Carpa Dorada/genética , Carpa Dorada/metabolismo , Hormona Liberadora de Gonadotropina/farmacología , Ovario/metabolismoRESUMEN
The aquatic environment is constantly exposed to chemical pollutants from agriculture and the urban environment. In this study, the effects of Bisphenol A, Naphthalene, and Butachlor on metabolic and antioxidant enzymes of goldfish were investigated during subacute exposure. The samples (n = 144, 100.97 ± 31.47 g) were distributed randomly in 12 glass aquaria (70 L) with a stocking density of 12 fish for each. Each aquarium was exposed to one of each pollutant (Bisphenol A with 500 µg/L, Butachlor with 0.28 µL/L and Naphthalene with 200 µg/L) separately, while the control group remained without contaminants for the entire 5 weeks of the experiment. The result showed that the activity of AST (Aspartate transaminase), ALT (Alanine transaminase), ALP (alkaline phosphatases), and POD (Guaiacol Peroxidase) significantly altered in contaminants treatments. The POD level, an antioxidant enzyme, showed a significant increase, especially on the 14th day of exposure to Bisphenol A (p < 0.05). In conclusion, these enzymes, as a biomarker, can be useful in environmental biomonitoring of aquatic ecosystems.
Asunto(s)
Contaminantes Ambientales , Contaminantes Químicos del Agua , Animales , Antioxidantes , Ecosistema , Carpa Dorada , Hígado , Contaminantes Químicos del Agua/toxicidadRESUMEN
Despite several studies on fish hormone therapy, finding new candidates may provide more reproductive efficiency in artificial propagation. Kisspeptins, being upstream of the hypothalamic-pituitary-gonadal axis, appear to play a key role in the reproduction process. In the present study, the effect of different variants of kisspeptide, including goldfish (Carassius auratus) kiss1 kisspeptin (Kiss1), human kisspeptin (Hkiss), and their combination (Kiss1 + H), on the reproductive indices of goldfish broodstock in comparison to Ovaprim (a typical synthetic Gnrh hormone) was investigated. Peptides (Kiss1 and Hkiss) were synthesized using a solid-phase synthesis approach. Kiss1 and Hkiss were injected at a dose of 100 µg kg-1 body weight, blood samples were taken 6 h after injection and sex hormones (E2, Dhp, and 11-Kt), gonadotropins (Lh and Fsh), cortisol and reproductive indices (fecundity, fertilization and hatching percentage) were measured. The results showed a significant increase of plasma sex hormones and gonadotropins in fish treated with kisspeptins. In addition, the cortisol and lipoprotein lipase in Kiss1, Hkiss and Kiss1 + H were remarkably increased compared to Ovaprim. In conclusion, kisspeptins could be a more suitable candidate than Ovaprim for accelerating and synchronizing oocyte maturation in the fisheries industry.
Asunto(s)
Carpa Dorada/fisiología , Kisspeptinas/farmacología , Reproducción/efectos de los fármacos , Animales , Domperidona/administración & dosificación , Domperidona/farmacología , Combinación de Medicamentos , Estradiol/sangre , Estradiol/metabolismo , Femenino , Hormona Folículo Estimulante/sangre , Hormona Folículo Estimulante/metabolismo , Hormona Liberadora de Gonadotropina/administración & dosificación , Hormona Liberadora de Gonadotropina/farmacología , Humanos , Hidrocortisona/sangre , Hidrocortisona/metabolismo , Hormona Luteinizante/sangre , Hormona Luteinizante/metabolismo , Masculino , Progesterona/sangre , Progesterona/metabolismo , Reproducción/fisiología , Testosterona/sangre , Testosterona/metabolismoRESUMEN
The application of equipment and tools that produce a magnetic field is increasing in aquatic ecosystems. In the present study, the effects of acute (1 week) and subacute (3 weeks) exposures to different static magnetic fields (SMFs) of 2.5, 5, 7.5 mT on stress indices (cortisol and glucose), sex steroid hormones (17ß-estradiol and 17-α hydroxy progesterone) and fecundity of the zebrafish (Danio rerio) were investigated. The obtained results showed a significant change in cortisol, glucose, 17ß-estradiol (E2) and 17-α hydroxy progesterone (17-OHP) levels by enhancing the intensity and time of exposure to SMFs. In conclusion, the SMFs, especially at higher levels of intensities, showed physiologically harmful effects on the reproductive biology of the zebrafish during acute and subacute exposures.