RESUMEN
The test for the evaluation of the toxicity of different types of pertussis preparations as manifested by their in vitro influence on mouse thymic cells (T test) has been finally worked out. The use of the T test has made it possible to reveal the nonstandard character of the production lots of adsorbed diphtheria-pertussis-tetanus vaccines, both whole-cell vaccine and Japanese acellular vaccine. The degree of the in vitro damaging action of pertussis preparations on mouse thymic cells greatly depends on the residual content of Bordetella pertussis nontoxoidized toxin which, in contrast to B. pertussis lipopolysaccharide and filamentous hemagglutinin, produces pronounced cytotoxic action on mouse thymic cells.
Asunto(s)
Vacuna contra la Tos Ferina/toxicidad , Timo/efectos de los fármacos , Animales , Peso Corporal/efectos de los fármacos , Células Cultivadas/efectos de los fármacos , Vacuna contra Difteria, Tétanos y Tos Ferina/toxicidad , Relación Dosis-Respuesta a Droga , Dosificación Letal Mediana , Ratones , Ratones Endogámicos CBA , Timo/citología , Factores de TiempoRESUMEN
The results of the trials of dried erythrocyte immunoglobulin immunodiagnosticum for the detection of hepatitis A virus antigen, developed by a group of scientists at the Tashkent Research Institute for Vaccines and Sera, are presented. The immunodiagnosticum is specific to hepatitis A virus antigen and suitable for the detection of this antigen in fecal and urine samples from patients with viral hepatitis A in the acute stage of the disease.
Asunto(s)
Antígenos Virales/análisis , Eritrocitos/inmunología , Hepatitis A/diagnóstico , Hepatovirus/inmunología , Inmunoglobulina G/análisis , Enfermedad Aguda , Epítopos/análisis , Estudios de Evaluación como Asunto , Heces/análisis , Hepatitis B/diagnóstico , Humanos , Pruebas Inmunológicas/métodos , Indicadores y ReactivosRESUMEN
The effectiveness of some immunological methods for diagnosing dysentery has been compared. This comparison has indicated that such methods as the determination of the presence of specific sensitization in the patients (by the indirect mast cell degranulation test), the detection of circulating complement-fixing antibodies to Shigella (by the complement fixation test) and antigenically reactive lymphocytes (by the lymphocyte agglutination test with the use of the antigen and by the indirect rosette-formation test) are more stable, expressive and sensitive than other serological tests and may be used for the complex clinical and laboratory diagnosis of this disease.
Asunto(s)
Disentería Bacilar/diagnóstico , Pruebas de Aglutinación , Pruebas de Fijación del Complemento , Gránulos Citoplasmáticos/metabolismo , Diagnóstico Diferencial , Gastroenteritis/diagnóstico , Pruebas de Hemaglutinación , Humanos , Linfocitos/inmunología , Mastocitos/metabolismo , Formación de Roseta , Pruebas Cutáneas , Fiebre Tifoidea/diagnósticoRESUMEN
Antiglobulin Coombs' test was included into the complex of sero-allergic reactions used for selection of the groups of population to be vaccinated by epidemiological indications, for the purpose of studying the incomplete antibodies in age aspect under conditions of the existing foci of brucellosis infection. A total of 1904 persons were examined. Incomplete antibodies were found with equal frequency in the blood serum of children and in adults of the population of the Kashka-Dariya region of Uzbek SSR residing in the foci of cattle brucellosis; it was revealed in 40.3% of children aged from 7 to 10 to 10 years, in 45.6% of children aged from 11 to 14 years, and in 42.3% of adults aged from 31 to 40 years.