RESUMEN

A 10 days Leucotrofina or Timolimfotropina administration induced in Wistar rats a rise of glycemia and liver glycogen content, and a decrease of glycogen-phosphorylase a activity. These modifications lasted in time and may be due to an activation of the hepatic gluconcogenetic pathway, via a stimulated glucocorticoid production.

Asunto(s)

RESUMEN

Adult male rats were treated for 10 days with a thymic extract (Leucotrofina-L, or Timolimfotropina-T, Ellem Milano, Italy), in a daily dose of 0.1 ml/100g body weight. 5 days after the end of the treatment, rats were sacrificed and liver was removed. The modifications in the liver were of the same direction for noth thymic extracts (an increase of the total protein content and of DNA concentration and a decrease of RNA concentration).

Asunto(s)

RESUMEN

Thymomodulin and Thymolymphotropin, biologically active thymus derivative peptides exert recovery effects on the functionality of some membrane bound, mitochondrial and lysosomal enzymes (monoamine oxidase, ATPase, phosphatases, cytochrome oxidase, succinate oxidase) affected by gamma-irradiation. These drugs exert antistress effect by re-establishing the function of hypothalamus-pituitary-adrenal axis and that of lymphoid organs.

Asunto(s)

RESUMEN

Leucotrofina administration in Wistar rats induced in thymus an increase of total protein content and of protein synthesis from 14C-leucine, simultaneously with a raise in the AlAT activity. Adrenal glands were not affected. Leucotrofina administration on the background of Cyclophosphamide induced modifications restored at normal values some of the thymic parameters (total protein content and RNA content) but the ascorbic acid content in the adrenals remained lowered.

Asunto(s)

RESUMEN

The influence of leukotrophine on the behaviour of lymphomyeloid organs was studied in male Swiss mice aged 2 months. In the four comparatively studied groups it was shown that leukopoiesis and immune maturation was slightly stimulated by leukotrophine or protected by thiola, a radioprotective substance; the administration of both substances, leukotrophine + thiola, enhances the two actions, myelo-lymphoid leukopoiesis and cellular pyroninophilia, almost doubling them. Histochemical studies were supplemented with histoenzymatic and cytogenetic investigations.

Asunto(s)

Asunto(s)

Asunto(s)

Asunto(s)

Asunto(s)

Asunto(s)

Asunto(s)

Asunto(s)

RESUMEN

Postirradiation administration of Leukotrophin to whole-body irradiated mice was associated with increased LD50/30 and DRF. As indicated by 59Fe uptake and ESC number, haemopoiesis was significantly stimulated in spleen and bone marrow after Leukotrophin application to irradiated mice. DNA content and the uptake of 3H-thymidine in DNA was significantly enhanced in the thymus and bone marrow of irradiated and Leukotrophin-treated mice. The micronucleus test confirmed that Leukotrophin is a therapeutic agents, while administered before irradiation it does not influence the initial radio-lesions.

Asunto(s)

RESUMEN

The paper aims at evidencing the stimulating effect on hematopoietic stem cells exerted by the thymus extract "Leukotrophin", prepared by Ellem Laboratory, Milan, Italy. Investigation of the hematologic and histologic parameters allowed the follow up of alterations occurring in bone marrow, blood and tissues of the irradiated animals, treated and untreated as compared with the unirradiated and untreated controls. Regeneration was more active in the groups of animals irradiated and treated with Leukotrophin than in the irradiated and untreated controls. A maximal regeneration of the myeloid series, prevalently of the erythrocytic line, was found 9 days after irradiation.

Asunto(s)

RESUMEN

Small intestine cell nuclei incubated in sucrose media released large fractions of DNA into the culture medium. This effect was partially or completely suppressed when incubation was carried out in the presence of a protease inhibitor, 10 to 30 mM NaHSO3. The DNA released in sucrose media containing NaHSO3 was precipitated as a DNA-protein complex by increasing the bivalent ion concentration to 10 mM Ca2+ or 20 mM Mg2+. Most of the released DNA was not precipitated by Ca2+ or Mg2+ when incubation was performed without NaHSO3. As determined by viscosity measurements the mean molecular weight of the DNA released in the absence of NaHSO3 was from 3.5-8.0 x 10(5) and increased to about 11 x 10(5) (corresponding to 8 nucleosomes) when the incubation mixture contained NaHSO3. End group analysis indicated that the DNA segments were terminated by 3'-OH groups. It is suggested that fragmentation of DNA in chromatin was produced by a endogenous alkaline endonuclease activity which was present in the fraction of released DNA. The data support the view that the third-order repeat structure of chromatin consists of subunits containing 8 nucleosomes.

Asunto(s)

Asunto(s)

Asunto(s)

Asunto(s)

RESUMEN

Thymic involution of Ehrlich ascites tumor bearing Swiss and NMRI mice was associated with an increased incorporation of 3H-thymidine into thymus DNA and an enhanced activity of adenosine deaminase. The apparent depletion time constants, which were determined by the following time course of the retention of 3H-DNA, indicated that the rate cell depletion was increased in the thymus of tumor bearing mice. The results suggest that the thymic involution was correlated with an increased proliferative activity and an accelerated depletion of thymic cells.

Asunto(s)

RESUMEN

During the period of 18 days after transplantation the uptake of 3H-thymidine into DNA was significantly increased in the spleen of Ehrlich ascites tumor-bearing Swiss and NMRI mice. Adenosine deaminase activity was within the control limits in tumor-bearing Swiss mice and significantly increased for the tumor-bearers of the NMRI strain. However, the increase in the DNA content, which is associated to splenic hyperplasia, was evident only for the Swiss strain. The time course of the retention of 3H-DNA revealed that the half life of spleen cells ranged from 41 to 46 h in normal and tumor-bearing Swiss mice and normal NMRI mice. For tumor-bearing NMRI mice the rate of cell depletion was significantly increased (t1/2 = 32h) compared with that found in normal NMRI mice (t1/2 =44 h). The mean survival time was 23.0 days for the tumor-bearing Swiss mice and 24.6 days for the NMRI mice. These data suggest that the immune responses were similar in both strains of mice, although cell kinetics was different in the spleen of tumor-bearing Swiss and NMRI mice.

Asunto(s)