RESUMEN
We studied the molecular mechanisms of cross-adaptation to ionizing radiation (1 Gy) of lymphocytes isolated from rats subjected to emotional stress. The effects of chronic (CES; various types of stress exposure) and acute (AES; forced swimming) emotional stress in rats on indicators of oxidative stress, cell death, and levels of NRF2 and NOX4 proteins involved in the development of the adaptive response were analyzed in isolated lymphocytes. It was found that stress induced an adaptive response in rat lymphocytes and triggered processes similar to the adaptive response induced by low doses of ionizing radiation: an increase in the level of oxidized DNA and cell death, as well as an increase in the content of NOX4 and NRF2 proteins. In animals subjected to emotional stress, suppressed DNA oxidation in response to irradiation, reduced levels of protective factor NRF2, as well as lymphocyte death were observed.
Asunto(s)
Linfocitos , Factor 2 Relacionado con NF-E2 , Estrés Oxidativo , Radiación Ionizante , Estrés Psicológico , Animales , Linfocitos/efectos de la radiación , Linfocitos/metabolismo , Ratas , Factor 2 Relacionado con NF-E2/metabolismo , Factor 2 Relacionado con NF-E2/genética , Estrés Psicológico/metabolismo , Masculino , Estrés Oxidativo/efectos de la radiación , Ratas Wistar , Adaptación Fisiológica/efectos de la radiación , NADPH Oxidasa 4/metabolismo , NADPH Oxidasa 4/genética , Daño del ADN/efectos de la radiaciónRESUMEN
The aims of the present work were to perform a comparative study of the effects of delta sleep-inducing peptide and Deltaran on neurons in emotiogenic brain structures and to address the question of whether it is possible to prevent or decrease the negative influences of stress loads on the severity of subsequent cerebral ischemia in rats, using glycine with delta sleep-inducing peptide combined in the neuroprotective formulation Deltaran. The results showed that Deltaran and delta sleep-inducing peptide had largely the same actions on the nature of spike activity of neurons in the dorsal hippocampus, paraventricular nucleus of the hypothalamus, and ventral anterior nuclei of the thalamus, evoking activation of some of the neurons in these brain structures. The dorsal hippocampus was dominated by activation of spike activity in response to administration of delta sleep-inducing peptide; Deltaran produced activation mainly in the paraventricular nuclei of the hypothalamus. In all animals given Deltaran, the index of brain blood supply was significantly greater than in animals not given Deltaran. The survival rate of cerebral ischemia was 100% in animals given Deltaran. Death occurred in 38% of animals not given Deltaran.
Asunto(s)
Isquemia Encefálica/prevención & control , Encéfalo/efectos de los fármacos , Péptido Inductor del Sueño Delta/farmacología , Glicina/farmacología , Neurotransmisores/farmacología , Estrés Psicológico/tratamiento farmacológico , Potenciales de Acción/efectos de los fármacos , Glándulas Suprarrenales/patología , Animales , Encéfalo/irrigación sanguínea , Encéfalo/fisiopatología , Isquemia Encefálica/complicaciones , Isquemia Encefálica/mortalidad , Combinación de Medicamentos , Hipocampo/efectos de los fármacos , Masculino , Tamaño de los Órganos/efectos de los fármacos , Núcleo Hipotalámico Paraventricular/efectos de los fármacos , Ratas , Ratas Wistar , Restricción Física , Estrés Psicológico/complicaciones , Estrés Psicológico/mortalidad , Estrés Psicológico/fisiopatología , Tálamo/efectos de los fármacos , Timo/patologíaRESUMEN
The characteristics of the actions of the non-competitive blocker of NMDA receptors dizocilpine on the expression of the early c-fos gene in the paraventricular nuclei of the hypothalamus were studied in rats with different predicted susceptibilities to emotional stress in conditions of treatment with delta sleep-inducing peptide. The results showed that prior treatment with dizocilpine blocked reductions in c-fos expression induced by delta sleep peptide.
Asunto(s)
Péptido Inductor del Sueño Delta/fisiología , Maleato de Dizocilpina/farmacología , Núcleo Hipotalámico Paraventricular/metabolismo , Proteínas Proto-Oncogénicas c-fos/biosíntesis , Receptores de N-Metil-D-Aspartato/antagonistas & inhibidores , Animales , Péptido Inductor del Sueño Delta/farmacología , Regulación de la Expresión Génica , Predisposición Genética a la Enfermedad , Inyecciones Intraperitoneales , Inyecciones Intraventriculares , Masculino , Núcleo Hipotalámico Paraventricular/efectos de los fármacos , Proteínas Proto-Oncogénicas c-fos/genética , Ratas , Ratas Wistar , Estrés Psicológico/genética , Estrés Psicológico/metabolismoRESUMEN
Emotional stress induced more marked increases in the expression of the c-fos gene in limbo-reticular structures of the brain in rats prognostically predisposed to emotional stress. I.p. doses of delta sleep-inducing peptide (DSIP) (60 nmol/kg) weakened the stress-induced expression of the c-fos gene. This effect was more apparent in animals predisposed to emotional stress, in which preliminary injections decreased stress-induced c-fos expression in the paraventricular hypothalamus and the medial and lateral parts of the septum. The decreased expression of the early gene c-fos in emotional stress after preliminary dosage with DSIP may reflect the leading mechanism of the anti-stress action of this peptide.