RESUMEN
The nucleotide sequence of the matrixprotein (M) gene of the lapinized rinderpest virus (RPV-L) was determined. The full-length cDNA of the RPV-L M gene is composed of 1460 base pairs and is supposed to contain an open reading frame of 1005 nucleotides encoding on M protein of 335 amino acids. The homology of the predicted amino acid among congeneric morbilliviruses such as RPV Kabete 'O' strain (wild strain of RPV), RPV RBOK strain (vaccine strain of RPV for cattle), measles virus (MV), and canine distemper virus (CDV), is approximately 94%, 93%, 87% and 77%, respectively. In the present study, all coding regions of the RPV-L strain have been determined.
Asunto(s)
Virus de la Peste Bovina/genética , Proteínas de la Matriz Viral/genética , Secuencia de Aminoácidos , Secuencia de Bases , ADN Viral/química , ADN Viral/genética , Genes Virales , Datos de Secuencia Molecular , Filogenia , ARN Viral/química , ARN Viral/genética , ARN Viral/aislamiento & purificación , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Peste Bovina/virología , Virus de la Peste Bovina/química , Homología de Secuencia de Aminoácido , Proteínas de la Matriz Viral/químicaRESUMEN
Forty Caspian seals were surveyed seroepidemiologically between 1993 and 1998 around the times of mass mortality that occurred in 1997 in the Caspian Sea and seven Baikal seals were also surveyed in 1998. Virus neutralizing tests and ELISA clearly suggested that distemper virus epidemic was caused in Caspian seals before the spring of 1997 and that CDV infection continued to occur in Lake Baikal in recent years.
Asunto(s)
Anticuerpos Antivirales/sangre , Brotes de Enfermedades/veterinaria , Virus del Moquillo Focino/inmunología , Infecciones por Morbillivirus/veterinaria , Phocidae/virología , Animales , Ensayo de Inmunoadsorción Enzimática/métodos , Ensayo de Inmunoadsorción Enzimática/veterinaria , Infecciones por Morbillivirus/epidemiología , Infecciones por Morbillivirus/virología , Federación de Rusia/epidemiología , Estudios Seroepidemiológicos , Siberia/epidemiologíaRESUMEN
The seroepidemiological survey of cats conducted in northern part of Taiwan in 1998 revealed that the positive rate of feline immunodeficiency virus (FIV)-infection was 21.9% (7/32) and the rate was much higher than those of previous reports. We succeeded in isolation of three strains of FIV from peripheral blood mononuclear cells of the blood samples. Nucleotide sequences of the env variable V3 to V5 region of the strains revealed that the isolates from distinct areas belong to subtype C. These data together with our previous report (Inada et al. 1997. Arch. Virol., 142: 1459-1467) indicate that FIV subtype C is prevalent in northern part of Taiwan.