RESUMEN
It is presented case report of diagnosis and treatment of foreign body of the intestine (magnets) in the 5-year-old child.
Asunto(s)
Cuerpos Extraños/diagnóstico , Cuerpos Extraños/terapia , Intestinos , Imanes/efectos adversos , Preescolar , Cuerpos Extraños/complicaciones , HumanosRESUMEN
Rabies virus nucleoprotein (N protein) encapsidates genomic RNA of the virus and forms the viral ribonucleoprotein complex. These N proteins represent highly organized structures which activate proliferation of B cells and production antibodies against the N protein. In addition to the B cell, the rabies virus N protein has been shown to induce potent T helper cell responses resulting in a long-lasting and strong humoral immune response. Rabies virus N protein is a molecular target of choice for development of tools to diagnose acute rabies infection. We produced a recombinant immune reactive C-terminal fragment of the rabies virus N protein which contains an antigenic determinant located between positions 360-389. Synthetic gene encoding the N protein was cloned into an expression plasmid to produce the recombinant antigen in Escherichiacoli cells BL21 (DE3). SDS-PAGE showed presence of the product with expected molecular weight (44 kDa). The recombinant fragment of the N protein efficiently recognized antibodies in sera from mice immunized with an inactivated rabies virus. Thus produced recombinant antigen of the rabies virus N protein can be used in an enzyme-linked immunosorbent assay (ELISA) for diagnosis of the rabies infection.
Asunto(s)
Sistema Nervioso Central/fisiopatología , Hipertensión Portal/fisiopatología , Derivación Portocava Quirúrgica , Niño , Estudios de Seguimiento , Encefalopatía Hepática/etiología , Encefalopatía Hepática/fisiopatología , Humanos , Hipertensión Portal/psicología , Hipertensión Portal/cirugía , Derivación Portocava Quirúrgica/efectos adversosRESUMEN
The authors have studied 22 patients with functioning vascular shunts (splenorenal--9, cavamesenterial--13) performed for extrahepatic portal hypertension in remote terms after operation (to 10.5 years). The vascular anastomosis in children was shown not to promote the development of considerable disturbances in functions of the liver and brain. It gives the authors a reason to recommend various vascular anastomoses in the treatment of children with the extrahepatic form of portal hypertension.