RESUMEN
OBJECTIVES: The Fueguians are descendants of the first settlers of America, a 'relict' isolated geographically for 10,000 years. We compared their cranial variation with other Americans, and samples from Asia and Australia to know whether the modern extinct Fueguians can be considered Paleoamericans or not. MATERIALS AND METHODS: Herein we study 176 Fuego-Patagonian skulls, the largest cranial sample to be studied, refined and well documented, using CVA, and the D2 of Mahalanobis. The affinities between populations and sexual dimorphism were jointly studied. RESULTS: Terrestrial hunters (Selknam) have a different cranial morphology from sea canoeists (Yamana, Alakaluf) particularly with regard to cranial size and robustness. In the American context, there are extreme differences between the canoeists of Santa Cruz (California) and the Eskimos and canoeists of Fuego-Patagonia in terms of cranial size, prognathism and development of the frontal region. Fueguian canoeists are cranially closer to the Californian ones than to their Fueguian neighbors, the Selknam. Our results favor the hypothesis of two different flows for the origin of the first populators of Tierra del Fuego. DISCUSSION: We concluded that the robusticity of some Fuegians (Selknam) might be the result of an allometric pattern of overall robusticity expression well as a result of epigenetics or differential reproduction (Larsen, 2015:264) or hypothetical endocrine changes (Bernal et al. in Am J Hum Biol 2006;18:748-765). When compared with three Australian-Melanesian series, the group comprising Amerindians, Ainu, and Eskimos clusters together as they are all extremely different from the former in terms of both cranial size and shape.
Asunto(s)
Etnicidad/estadística & datos numéricos , Indígenas Sudamericanos/estadística & datos numéricos , Cráneo/anatomía & histología , Adulto , Antropología Física , Cefalometría , Chile , Femenino , Humanos , Masculino , Caracteres Sexuales , Cráneo/patología , Población BlancaRESUMEN
BACKGROUND: Movima, Yuracare, Ignaciano and Trinitario are Amerindian populations living in the Bolivian lowlands of the Amazonian basin. The cultural and genetic affinity of the peoples living in this area is poorly known, despite many archaeological studies demonstrating its importance in pre-Columbian times. Densely populated Amerindian groups occupied the region, both in the Llanos and along the river streams of the Amazonian basin, practising intense agricultural activities and exchange of goods. The historical and linguistic records indicate that the land was occupied through successive migrations that gave rise to complex socio-economic communities. Genetic information suggests that the colonization of the American continent was fairly simple from a emigrational point of view, but other evolutionary processes, such as genetic drift or natural selection, could have also shaped the genetic background of present day populations in the Beni region. AIM: The objective of this study is to characterize the genetic diversity of these populations by analysing the sequence variability of the HVR-I control region in the mitochondrial DNA (mtDNA). The Amerindian origin of these populations suggests that close genetic similarities should be evident between the Beni samples studied here and other Amerindian groups. However, complex processes of population interactions and/or isolation in the Beni region might result in non-expected genetic affinities. SUBJECTS AND METHODS: DNA was extracted from pulled-out hairs obtained in situ from non-closely related individuals living in the Beni Department in Bolivia. DNA was extracted using a standard Chelex 100 method and a 401 bp DNA fragment of the HVR-I region was amplified using specific primers (L-15978 and H-16412). DNA amplicons were purified by centrifugation using Microspin S-300 HR columns and both SNA strands were sequenced after asymmetric PCR using direct Dye-Terminator 2 sequencing kit (Perkin-Elmer). Two independent 401 and 328 bp DNA fragments were sequenced separately for each sample. The sequence analyses includes mismatch distributions and mean pairwise differences, median network analysis, and neighbour joining, maximum likelihood phylogenetic comparisons. Genetic diversity of DNA sequences was also measured in various ways for the sample studied and UPGMA trees were drawn, including a large number of South Amerindian sequences. RESULTS: The genetic diversity of 401 nucleotide long mtDNA sequences in the hypervariable control region, from positions 16,000-16,400, was characterized in a sample of 54 Amerindians living in the Llanos de Moxos. A total of 34 distinct lineages were observed, defined by 41 variable nucleotide positions, and 70.6% of all lineages were single sequences. All four major Amerindian haplogroups were detected (A 18.5%, n=10; B 24.1%, n=13, C 50.0% n=27; and D 5.6%, n=3). The median network analysis observed suggests that processes of population expansion took place in the Beni region. However, no clear haplotype differentiation by population could be detected. High levels of molecular variability and a bimodal pair-wise mismatch distribution were seen within the sample. The analyses of molecular variance (AMOVA) showed that most of the variance observed was due to intrapopulation variability, and that the highest among-groups variance was obtained when a linguistic classification criteria was used. The phylogenetic comparison revealed unique lineages in the Beni areas, not reported for other Amerindian populations. CONCLUSIONS: The genetic diversity observed in the Beni area is higher than that observed in other American populations living in much larger areas and with a long, known evolutionary history, despite the reduced area of Moxos. This could result from processes of reproductive isolation between groups, followed by population expansions and migration, where genetic drift might have be a major evolutionary force in population differentiation.
Asunto(s)
ADN Mitocondrial/genética , Variación Genética , Genética de Población , Indígenas Sudamericanos/genética , Bolivia , Humanos , Filogenia , Polimorfismo Genético , Análisis de Secuencia de ADN/métodosRESUMEN
This study provides the frequencies of four mitochondrial DNA (mtDNA) haplogroups of 233 native South Amerindians in eight populations living in the Beni Department of Bolivia, including six populations not previously studied. Linguistically, these populations belong to the three principal South Amerindian language stocks, Andean, Equatorial-Tucanoan, and Ge-Pano-Carib. Frequency analyses under geographic, historic, linguistic, and genetic configurations using the theta statistic of Weir (Weir 1990) and analysis of molecular variance (AMOVA) show similar results. Results are also similar when phenetic cluster is used. Aymara belongs almost exclusively to haplogroup B, Quechua- and Moseten-speaking tribes belong to haplogroups A and B, but the first tribe presents high frequencies of haplogroup B. Yuracare, Trinitario, and Ignaciano exhibit high frequencies of A, B, and C haplogroups, and the Movima present a large proportion of haplogroup C. There is some correspondence between mtDNA haplogroup frequencies and language affiliation and historical connections, but less so with geographic aspects. The present study provides a context for understanding the relationship between different Amerindian populations living in a multiethnic area of Bolivia.
Asunto(s)
ADN Mitocondrial/genética , Heterogeneidad Genética , Haplotipos/genética , Indígenas Sudamericanos/genética , Análisis de Varianza , Bolivia , Variación Genética , Humanos , Lenguaje , Polimorfismo GenéticoRESUMEN
Ancient DNA from bones and teeth of 60 individuals from four extinct human populations from Tierra del Fuego-Patagonia (Selknam, Yamana, Kaweskar and Aonikenk) has been extracted and the mitochondrial DNA (mtDNA) amplified by using the polymerase chain reaction. High-resolution analysis of endonuclease restriction site variation in the mtDNA and sequencing of its hypervariable non-coding control region, revealed complete absence of two of the four primary mitochondrial haplotype groups present in contemporary Amerinds, namely A and B. In contrast, haplogroups C and D were found in all but one sample with frequencies of approximately 38% and 60%. These results, together with the decreasing incidence of group A in more southerly latitudes in the American continent and the absence of cluster B above 55 degrees North in America and Asia, argue that the first settlers entering America 21000-14000 years ago already lacked both mtDNA lineages.