RESUMEN
OBJECTIVE: To study the anti-asthmatic effects of Volatile oil of Radix Angelicae Sinensis (VOR, Traditional Chinese Medicine) on asthmatic BALB/c mice and its effect on Th17 cell immuno-activity through IL-17A and RORγt. METHODS: After grouping (n=12), the asthmatic BALB/c mice were replicated through injection of ovalbumin (OVA) for sensitization and administration of OVA aerosol for challenge and then, the asthmatic behaviors, respiratory function, lung histopathology as well as levels of IL-17A in serum and retinoid-related orphan receptor gamma t (RORγt) in lung tissue were observed after the action of VOR. RESULTS: VOR could maintain normal growth of body weight in asthmatic mice, improve asthmatic behaviors, respiratory function, lung histopathology and, inhibit over-expression of IL-17A and RORγt (P<0.05, 0.01) at 60, 120, 240 mg/kg doses. The combination of VOR and dexamethasone could bring synergistic effects on growth of body weight and expression of IL-17A and RORγt. CONCLUSIONS: VOR has significant effects of anti-asthma and one of the mechanisms is to inhibit immune activity of Th17 cell through relieving over-expression of IL-17 and RORγt. Besides, the combination of VOR and glucocorticoid could bring synergistic effects.
Asunto(s)
Antiasmáticos/farmacología , Asma/tratamiento farmacológico , Medicamentos Herbarios Chinos/farmacología , Aceites Volátiles/farmacología , Células Th17/efectos de los fármacos , Angelica sinensis , Animales , Líquido del Lavado Bronquioalveolar , Interleucina-17/sangre , Pulmón/patología , Ratones , Ratones Endogámicos BALB C , Miembro 3 del Grupo F de la Subfamilia 1 de Receptores Nucleares/metabolismo , Ovalbúmina , Células Th17/inmunologíaRESUMEN
OBJECTIVE: To investigate the protective effects of Yuyin Ruangan Decoction(YRD, traditional Chinese medicine) on experimental hepatic injury in mice. METHODS: The mice were randomly divided into control group, model group and YRD low, middle and high dose group(n = 11). By ip injection of D-GalN, CCk or thioacetamide (TAA), three models of hepatic injury mice were established to investigate the effects of YRD through detecting the indexes of liver function in serum and, the content of antioxidant system in the hepatic tissue. RESULTS: YRD could decrease the content of alanine aminotransferase (ALT)and aspartate aminotransferase (AST) in serum and that of malonaldehyde (MDA) in the hepatic tissue, upregulate the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the hepatic tissue. Furthermore, the above effects were dosedependent in a certain degree. CoNCLUSION: YRD has some protection effects on the model of experimental hepatic injury in mouse.
Asunto(s)
Enfermedad Hepática Inducida por Sustancias y Drogas/tratamiento farmacológico , Medicamentos Herbarios Chinos/farmacología , Alanina Transaminasa/sangre , Animales , Antioxidantes/metabolismo , Aspartato Aminotransferasas/sangre , Glutatión Peroxidasa/metabolismo , Hígado/efectos de los fármacos , Hígado/enzimología , Malondialdehído/metabolismo , Ratones , Superóxido Dismutasa/metabolismoRESUMEN
OBJECTIVE: To study the antalgic and antiphlogistic functions and mechanism of ronggudingtong (RGDT) plaster (traditional Chinese medicine). METHODS: The painful models were established with hot plate test or acetic acid writhing and the inflammatory models were established with daubing dimethylbenzene on auricle or injecting formaldehyde in toe or synovial envelope to study the antalgic and antiphlogistic functions of RGDT Plaster. The total protein and leukotriene B4(LTB4) in inflammatory exudate were detected to investigate the antalgic and antiphlogistic mechanism of RGDT plaster. The mice were randomly divided into different groups (n = 11), on the basis of drug using, the indexes of pain threshold, swelling degree were observed. Sixty-six mice were used to establish gasbag synovitis model and randomly divided into normal control group,model control group, positive control group (Voltaren gel 0.8 mg/d)and low/medium/high dosage RGDT plaster treating groups(30 mg/d, 60 mg/d, 120 mg/d). RESULTS: 30 mg/d, 60 mg/d,120 mg/d RGDT plaster could upgrade the pain thresholds, remit auricular and foot swelling (P < 0.05, P < 0.01), and degrade total protein and LTB4 in inflammatory exudates (P < 0.05, P < 0.01). CONCLUSION: RGDT plaster has some antalgic and antiphlogistic functions, and one of the mechanisms is depressing synthesis of LTB4.
Asunto(s)
Analgésicos/farmacología , Antiinflamatorios/farmacología , Dolor/tratamiento farmacológico , Animales , Leucotrieno B4/metabolismo , Medicina Tradicional China , RatonesRESUMEN
OBJECTIVE: To establish and evaluate a mouse model of bronchial asthma with Yin deficiency syndrome. METHODS: The mouse model of bronchial asthma with Yin deficiency syndrome was established by the treatment with injecting ovalbumin (OVA) two times to sensitize, inhaling OVA 14 times to stimulate, and using thyroxin through lavage during late stimulation. This model was evaluated through body weight, asthmatic behaviors, respiratory function, autonomous activity, lung pathology, and pulmonary fluid clearance. RESULTS: OVA combined with thyroxin was an appropriate method to induce the mouse model with increased food and water intake, autonomous activity, asthmatic behaviors score, and respiratory rate, decreased body weight, tidal volume, and wet/dry ratio of lung, and changed with pathology of lung tissue. The changes of the above mentioned parameters indicated that the model was the bronchial asthma with Yin deficiency syndrome. CONCLUSION: The OVA combined with thyroxin is a good pattern to establish a mouse model of bronchial asthma with Yin deficiency syndrome successfully, which can highly simulate the clinical symptoms of this disease.