RESUMEN

Two homogeneous, metallic ion-containing pectic polysaccharides with mean M(r)s of 7600 and 15,000 were isolated from dried aerial parts of Achyrocline satureioides by anion exchange column chromatography on DEAE-Sepharose CL-6B and gel filtration column chromatography on Fractogel TSK HW-50 (S). The structures, as determined by methylation analysis, carboxyl reduction, and partial acid hydrolysis, were shown to be rhamnogalacturonans. Both pectins show a pronounced anticomplementary effect in vitro. The larger carbohydrate AS 4 of higher M(r) exerts anti-inflammatory activity and a strong enhancement of phagocytosis in vivo.

Asunto(s)

Pectinas/aislamiento & purificación , Plantas Medicinales/química , Secuencia de Carbohidratos , Proteínas Inactivadoras de Complemento/química , Proteínas Inactivadoras de Complemento/aislamiento & purificación , Metales/química , Datos de Secuencia Molecular , Pectinas/química , Pectinas/inmunología , Plantas Medicinales/inmunología