RESUMEN
It is shown that in using the method of multiple organ cultivation, the tissues of the CNS in the human and animal embryo preserve their organotypical characteristics. The use of a cartilage plate increases the viability of the tissues and the duration of its maintenance in cultures. During the process of explantation the cultivated tissue undergoes some morphological modifications. The data obtained are discussed in the light of a possible use of the CNS cultures for experimental purposes.
Asunto(s)
Sistema Nervioso Central/anatomía & histología , Tejido Nervioso/anatomía & histología , Animales , Encéfalo/anatomía & histología , Cercopithecus , Haplorrinos , Humanos , Ratones , Técnicas de Cultivo de Órganos , Ratas , Médula Espinal/anatomía & histología , Factores de Tiempo , Supervivencia TisularRESUMEN
A morphologic study of 57 cancers of the human gastrointestinal tract maintained in short-term organ culture was carried out, and the response of part of these tumors to infection with poliovirus was studied. The tumors, grown in vitro, mainly retained their original characteristics and histologic types. A certain correlation between the histologic type and its viability in short-term organ culture was revealed: the more differentiated forms were maintained better than the poorly differentiated ones. A comparative study of the polio-virus-infected and the control cultures indicated that wild poliovirus showed the most marked oncolytic activity in short-term organ culture, whereas attenuated poliovirus III produced a less marked, although still distinct, destructive effect.