RESUMEN
Haloperidol is widely used in psychiatric practice both in monotherapy and in combination with neuroleptics, phenothiazine derivatives (chlorpromasin, levomepromasin) and antidepressants (amitriptilin, imipramin). We have developed techniques of haloperidol isolation from blood in combined poisonings with phenothiazine derivatives (chlorpromazine, levomepromasine) and antidepressants (amitriptilin, imipramin), their identification and quantitation with high-performance liquid chromatography. The highest output of the substances studied was achieved in alkaline hydrolysis. The techniques are expressive, sensitive and well reproducible. This makes them convenient for practice of forensic-chemical and chemico-toxicological laboratories.
Asunto(s)
Antipsicóticos/sangre , Toxicología Forense/métodos , Haloperidol/sangre , Intoxicación/sangre , Cromatografía Líquida de Alta Presión , Humanos , Intoxicación/etiología , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Factores de TiempoRESUMEN
To optimize conditions of tiapride isolation from cadaveric organs, we compared the results of conventional methods by Stas-Otto, A.A. Vasilyeva and V.F. Kramarenko which provide tiapride isolation up to 50% and a new precise and reproducible method providing 60 +/- 2% tiapride isolation. Identification of tiapride isolated from cadaveric material was made with thin-layer chromatography and high performance liquid chromatography. The latter assay employed the method of external standard. The original techniques proposed identify and measure tiapride in hepatic samples in the presence of unidentified endogenic compounds. The techniques are rapid, selective, sensitive and reproducible.
Asunto(s)
Antipsicóticos/análisis , Toxicología Forense/métodos , Clorhidrato de Tiapamilo/análisis , Cadáver , Toxicología Forense/normas , Humanos , Sensibilidad y EspecificidadRESUMEN
Offers methods for isolation of lidocaine and novocainamide present together in the urine, blood plasma, and saliva. Demonstrates the possibility of identifying these substances in biological fluids by thin-layer and gas liquid chromatography. Proposes methods for measuring lidocaine and novocainamide in biological objects by UV spectrometry (combined polynomial) and gas liquid chromatography. Demonstrates the reliability of the results.
Asunto(s)
Antiarrítmicos/análisis , Líquidos Corporales/química , Lidocaína/análisis , Procainamida/análisis , Antiarrítmicos/aislamiento & purificación , Cromatografía de Gases/métodos , Cromatografía de Gases/estadística & datos numéricos , Cromatografía en Capa Delgada/métodos , Cromatografía en Capa Delgada/estadística & datos numéricos , Humanos , Procainamida/aislamiento & purificación , Espectrofotometría Ultravioleta/métodos , Espectrofotometría Ultravioleta/estadística & datos numéricosRESUMEN
A method for spectrophotometric measurement of metronidasole and ethymisole jointly present in the urine has been developed. Combined polynomes have been designed to eliminate the effects of light-absorbing admixtures extracted from the urine on the results of analysis.
Asunto(s)
Etimizol/orina , Metronidazol/orina , Espectrofotometría Ultravioleta/métodos , Cromatografía Líquida de Alta Presión , Cromatografía en Capa Delgada , Humanos , Concentración de Iones de Hidrógeno , Espectrofotometría Ultravioleta/estadística & datos numéricosRESUMEN
Optimal conditions of metronidazole extraction from aqueous solutions by different solvents using a mathematical method of experiment planning (latin square) were studied. It was stated that optimal conditions of metronidazole extraction from aqueous solutions were: extraction time (5 min.), pH medium-6, extractant-isoamyl spirit in saturation of aqueous phase with ammonium sulfate.