RESUMEN
The usefulness of the ATP bioluminescence method for monitoring surface hygiene was evaluated in a hospital pharmacy cleanroom. The sensitivity of the method was found to be appropriate for assessing the efficiency of cleaning and disinfection. ATP bioluminescence was superior to the traditional microbiological culture-based method for detecting unclean surfaces (pâ¯<â¯.05).
Asunto(s)
Adenosina Trifosfato/análisis , Desinfección/métodos , Desinfección/normas , Ambiente Controlado , Mediciones Luminiscentes/métodos , Servicio de Farmacia en Hospital , Bacterias/efectos de los fármacos , Recuento de Colonia Microbiana/estadística & datos numéricos , Desinfectantes/farmacología , Contaminación de Equipos/prevención & control , Propiedades de SuperficieRESUMEN
Knowing the normal cleanroom microbiota is the basis for ensuring microbiological quality; assess changes and the introduction of new sampling methods. During our study, we prepared a catalogue of cleanroom microorganisms located in four different cleanrooms in University Clinical Centre Ljubljana Pharmacy. Catalogue is prepared as a basis for assessing the suitability of the new rapid microbiological method and subsequent correlation of the results of both methods. The results of our study showed that 78% of isolated bacteria are Gram-positive. However, in more than 70% isolated bacteria were the part of the normal human microbiota, 10-15% of the microorganisms originated from the air, mainly spore-forming bacteria of the genus Bacillus and fungi, and 5-10% of the Gram-negative microorganisms that originated from the water and represent the potential endotoxins (pyrogens).