RESUMEN
The production of river buffalo meat in Italy has long been under discussion due to poor acceptance by consumers. In order to understand whether dietary energy content may affect the organoleptic characteristics of buffalo meat, two groups of river buffalo calves were fed on two diets, with high (H) and low (L) energy contents. The animals were slaughtered at 4-monthly intervals starting from 6 months old (10, 14 and 18 months) and five muscles were dissected on the half-carcass: Caput longum tricipitis brachii (CloTB), Gluteobiceps (Gb), Semitendinosus (St), Semimembranosus (Sm) and Longissimus dorsi (LD). The results showed that from 6 to 10 months of age the meat lipid content decreases and protein content increases for both diets. The lipid content increases slowly with both diets from 10 to 14 months. In the last experimental period (from 14 to 18 months) an increase in the percentage of lipids with diet H and a decrease with diet L was observed. At all slaughtering ages the meat from the animals fed on diet H had a higher energy content. The different energy content of the two diets did not significantly influence the composition of triglycerides only formed by SFA and triglycerides with a higher degree of unsaturation. The triglycerides with an unsaturated fatty acid in position sn 2 did not show the same behaviour in relation to age and diet. The energy content of the feed did influence the unsaturated fatty acid composition: MUFA increased with an increased energy level of the diet, while PUFA increased with a reduction in the energy level of the diet. The muscle LD showed a significantly higher (P<0.05) content of SFA and lower (P<0.05) of MUFA and PUFA than the other muscles. On the basis of our results, the better TAG's composition is found in the meat of animals fed on diet H and slaughtered at 4 months of age.
RESUMEN
Triacylglycerols from cows and buffaloes' milk fat were investigated by 13C nuclear magnetic resonance (NMR) spectroscopy. By the addition of pure triacylglycerols standards, we identified the resonances of both milk fats, and the peaks were used for qualitative and quantitative analysis of acyl groups. Multivariate analysis treatment of triacylglycerols distribution and composition parameters enabled us to identify milk. This study shows that NMR can safely be used to quantitate milk fatty acid content, providing unique information for milk identification of different animal species.
Asunto(s)
Leche/química , Triglicéridos/análisis , Animales , Búfalos , Isótopos de Carbono , Bovinos , Femenino , Espectroscopía de Resonancia Magnética , Análisis Multivariante , Especificidad de la EspecieRESUMEN
Three new N-acyl-2-methylene-beta-alanine methyl esters, Hurghamides E-G (5-7), were isolated from a Red Sea sponge Hippospongia sp. Their structures were elucidated by extensive spectroscopic studies.
Asunto(s)
Alanina/análogos & derivados , Alanina/química , Ésteres/química , Poríferos/química , Alanina/aislamiento & purificación , Animales , Ésteres/aislamiento & purificación , Océano Índico , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Solventes , Espectrofotometría InfrarrojaRESUMEN
Three novel fatty acid derivatives (1-3), containing one or two butenolide moieties, were isolated from the Caribbean gorgonian Pterogorgia anceps and chemically characterized by spectroscopic methods and comparison with known compounds. The new molecules were structurally related to ancepsenolide (4), a typical metabolite from Pterogorgia species, which was not detected in this collection of P. anceps.
RESUMEN
Oxidation of low density lipoprotein (LDL) has been implicated in atherogenesis. An increased content of oleic acid in LDL and the substitution of monounsaturated for polyunsaturated fatty acids in the diet reduce LDL oxidation. With 1H-NMR analysis, all LDL modifications, including the production of copper-induced aldehyde products, can be evaluated simultaneously. The aim of the present study was to investigate whether the fatty acid composition of LDL affected the NMR evaluation of aldehyde compounds. The LDL of the samples utilized were rich in oleic fatty acid (26.9%). After 48 h of exposure to copper sulfate, the mean production of malonyldialdehyde (MDA) by LDL was 31.2 nmol/mg of protein. Moreover, in the present study NMR did not reveal large amounts of peroxidative compounds since the nanomolar amounts of MDA produced after exposure to copper sulfate could not be detected. This study also demonstrated that the fatty acid composition (i.e. the oleic:linoleic acid ratio) must be taken into account in the evaluation of LDL peroxidation by NMR. In particular, a high concentration of oleic acid may limit the formation of large amounts of peroxidative compounds generated after exposure to the oxidant copper sulfate.
Asunto(s)
Sulfato de Cobre/farmacología , Ácidos Grasos/química , Peroxidación de Lípido/efectos de los fármacos , Lipoproteínas LDL/química , Lipoproteínas LDL/farmacología , Adulto , Ácidos Grasos/sangre , Humanos , Lipoproteínas LDL/sangre , Espectroscopía de Resonancia Magnética , MasculinoRESUMEN
The structural elucidation of the polar lipids in Halobacterium trapanicum is reported with particular emphasis on a new sulfated disaccharide derivative of 2,3-di-O-phytanyl-sn-glycerol. The full structural designation of this glycolipid is 2,3-di-O-phytanyl-1-O- (mannopyranosyl-(2-sulfate)-alpha-D-1-2-glucopyranosyl-alpha-D)-sn-glyce rol. The value of glycolipid structures in the taxonomy of halophilic Archaea is also discussed.
Asunto(s)
Disacáridos/química , Glucolípidos/química , Halobacterium/química , Secuencia de Carbohidratos , Clasificación , Disacáridos/aislamiento & purificación , Glucolípidos/aislamiento & purificación , Halobacterium/clasificación , Espectroscopía de Resonancia Magnética , Datos de Secuencia Molecular , Estructura Molecular , Fosfolípidos/química , Fosfolípidos/aislamiento & purificaciónRESUMEN
ThreeHypselodoris species,H. villafranca, H. cantabrica, and H. tricolor, have been studied in order to further investigate the defensive strategies of Chromodorididae mollusks from Iberian coasts. All animals possess large amounts of furanosesquiterpenoids, probably derived from their diet of sponges. The products are transferred and stored in specific sites of the mantle (mantle dermal formations, MDFs) to be used as chemical deterrents against predators. Chemical analysis of CantabrianHypselodoris species has led to the characterization of eight sesquiterpenoids. Among these, six have been found previously in both sponges and nudibranchs, while two are new natural products.Hypselodoris species cooccurring in the same area contained comparable mixtures of sesquiterpenoids. However, two populations ofH. cantabrica from different sites of the Cantabrian sea showed different metabolic patterns. All sesquiterpenoids induce feeding deterrence inCarassius auratus and two are also toxic toGambusia affinis.
RESUMEN
1. The Mediterranean sponge Reniera sarai, Pulitzeri-Finali, 1969 (Demospongiae: Haploscleridae: Renieridae) possesses in large amounts a series of unprecedented polycyclic alkaloids, saraines 1-3 and saraines A-C. 2. The structural peculiarities of saraines, their chemical-physical characteristics, along with their relevant abundance in the sponge, prompted a study aimed at investigating their biological properties. 3. Saraines were assayed for their cytotoxic, antibacterial, insecticidal and potential antitumoral activities. These results, along with the growth inhibition of fertilized sea urchin eggs, are reported.
Asunto(s)
Alcaloides/farmacología , Poríferos/química , Aedes , Agrobacterium tumefaciens , Animales , Artemia/efectos de los fármacos , Artrópodos , Insecticidas , Ácaros , Tumores de Planta , Erizos de Mar , Staphylococcus aureus/efectos de los fármacos , Cigoto/efectos de los fármacos , Cigoto/crecimiento & desarrolloRESUMEN
The conformational flexibility of the [Thr6, Leu13 psi(CH2NH) Met14] bombesin (6-14) nonapeptide has been studied by CD and one- and two-dimensional (1D and 2D) nmr techniques. The CD and nmr parameters in different solvents and in a micellar environment (SDS) are compared with the data collected for the parent bombesin (BN) and [D-Phe12, Leu14]BN. A preliminary investigation on spantide is also reported. In particular, the results obtained from CD measurements indicate that there is a shift from random coil structures, in aqueous solutions, toward folded structures in apolar media (2,2,2-trifluoroethanol) and in a membrane-mimetic environment (40 mM SDS) for all three peptides, namely BN, [D-Phe12, Leu14]BN, and [Thr6, Leu13 psi(CH2NH) Met14]BN (6-14). Spantide, which also possesses some inhibitory activity against BN but very little sequence similarity, even in water, shows an ordered conformation. Nuclear magnetic resonance parameters such as backbone NH-alpha CH coupling constant values, amidic temperature coefficients, and the presence of only sequential nuclear Overhauser effects have not provided, so far, any clear evidence for a preferential ordered structure in the peptides studied, and this may be due to rapid exchange among different conformers in the nmr time scale.
Asunto(s)
Bombesina/análogos & derivados , Bombesina/antagonistas & inhibidores , Oligopéptidos/química , Fragmentos de Péptidos/química , Secuencia de Aminoácidos , Bombesina/química , Dicroismo Circular , Espectroscopía de Resonancia Magnética/métodos , Datos de Secuencia Molecular , Conformación ProteicaRESUMEN
The conformational properties of spantide [(D-Arg, D-Trp, Leu) substance P] have been studied by fluorescence, CD, and nmr techniques. The fluorescence, CD, and nmr parameters in different solvents and in a micellar environment (SDS) are compared with the data collected for bombesin. A preliminary investigation on [D-Pro] spantide is also reported.
Asunto(s)
Bombesina/química , Sustancia P/análogos & derivados , Secuencia de Aminoácidos , Dicroismo Circular , Espectroscopía de Resonancia Magnética , Datos de Secuencia Molecular , Soluciones , Espectrometría de Fluorescencia , Sustancia P/químicaRESUMEN
Hypselodoris nudibranchs from different geographic areas (Spain and Italy) have been studied in order to investigate their general defensive strategy. Longifolin (1) and nakafuran-9 (2) are the main ichthyodeterrent allomones used by the mollusks to avoid predation. Evidence of their dietary origin is presented and the very effective strategy against predators, which includes secretion of allomones into the mucus and their storage into specific mantle dermal formations (MDFs), is also discussed.
RESUMEN
The title compound 3, an amatoxin analogue containing L-alpha-aminobutyric acid instead of L-asparagine in position 1, as in natural toad stool peptides, has been synthesized. It does not inhibit the eukaryotic DNA-dependent RNA polymerase form II (or B) in concentrations up to 10(-4)M, whereas 50% inhibition is exerted in 10(-6)M solution by the corresponding Asn-analogue S-deoxo-Ile3-amaninamide 2. The striking difference seems to be due to a relatively small variation of the conformation recognized by sensitive NMR spectroscopic methods.
Asunto(s)
Amanitinas , Amanitinas/síntesis química , Amanitinas/farmacología , Secuencia de Aminoácidos , Dicroismo Circular , Espectroscopía de Resonancia Magnética , Datos de Secuencia Molecular , Estructura Molecular , Conformación Proteica , ARN Polimerasa II/antagonistas & inhibidores , TemperaturaRESUMEN
The conformation flexibility of the tetradecapeptide hormone bombesin and its synthetic antagonist (DPhe12, Leu14)-bombesin has been studied using nuclear magnetic resonance and circular dichroism techniques. The spectral features observed indicate that the ordered structure present in the C-terminal pentapeptide moiety of native BBS is lost in the (DPhe12, Leu14) analog.
Asunto(s)
Bombesina , Bombesina/análogos & derivados , Bombesina/metabolismo , Bombesina/farmacología , Dicroismo Circular , Espectroscopía de Resonancia Magnética , Conformación Proteica , Receptores de Bombesina , Receptores de Neurotransmisores/efectos de los fármacos , Receptores de Neurotransmisores/metabolismo , Relación Estructura-ActividadRESUMEN
The conformational behavior of a heterodetic bicyclic decapeptide (BCPLT) in the absence and in the presence of calcium ions has been studied by means of mono and two-dimensional nmr techniques. Free BCPLT possesses a quite compact structure stabilized by intramolecular bonds and turns. In the structure a cluster of carbonyls is located in a cavity that is supposed to be the cation binding site.
Asunto(s)
Calcio , Péptidos Cíclicos , Conformación Proteica , Secuencia de Aminoácidos , Modelos Moleculares , Datos de Secuencia Molecular , Unión ProteicaRESUMEN
The proton nmr characterization of bombesin (BBS) and of two peptide fragments corresponding to the (1-6) and (6-14) sequences has been carried out at 500 MHz in dimethyl sulfoxide (DMSO-d6) using two-dimensional (2D) homo and 1H-13C heterocorrelated techniques. All resonances in the nmr spectra have been assigned and several coupling constants have been measured. The backbone J alpha CH-NH coupling constants are quite similar and around 7.8-8.2 Hz, pointing to an unfolded structure in DMSO-d6. The possibility of secondary structures in highly viscous mixtures of DMSO-d6-water was investigated. The existence of sequential nuclear Overhauser enhancement (NOE) effects in the C-terminal nonapeptide section may indicate a preferential site for secondary structuring.
Asunto(s)
Bombesina , Oligopéptidos , Bombesina/síntesis química , Dimetilsulfóxido , Espectroscopía de Resonancia Magnética , Oligopéptidos/síntesis química , Conformación Proteica , AguaRESUMEN
The proton NMR characterization of bombesin has been carried out at 500 MHz in DMSO-d6 using two-dimensional homo- and 1H-13C hetero-correlated techniques. All resonances in the NMR spectra have been assigned and several coupling constants have been measured. The backbone J alpha CH-NH coupling constants have constant values that vary between 7.8 and 8.2 Hz and indicate an unfolded structure in DMSO-d6. Discrepancies with data recently obtained at 300 MHz [(1987) Eur. J. Biochem. 168, 193-199] are discussed.
Asunto(s)
Bombesina , Secuencia de Aminoácidos , Bombesina/síntesis química , Deuterio , Dimetilsulfóxido , Espectroscopía de Resonancia Magnética/métodos , Conformación ProteicaAsunto(s)
Contracción Muscular/efectos de los fármacos , Músculo Liso/fisiología , Oligopéptidos , Animales , Cobayas , Técnicas In Vitro , Espectroscopía de Resonancia Magnética/métodos , Músculo Liso/efectos de los fármacos , Oligopéptidos/farmacología , Péptidos Opioides , Relación Estructura-ActividadRESUMEN
Amatoxin analogues with D and L-Ala substitutions in position 5 have been studied by means of 1- and 2-dimensional n.m.r. spectroscopy at 500 MHz. The assignment of all resonances for both analogues has been carried out mostly with the use of COSY and NOESY type experiments. Temperature coefficients for the amide NH protons have been measured and the data compared to known amatoxin structures. The results obtained demonstrate that the rigidity of the bicyclic amatoxin framework is preserved in the D and L-Ala5 analogues, although the temperature coefficients point to intramolecular hydrogen bonds stronger in the case of the L-Ala analogue. The 10-fold decrease of biological activity is discussed in terms of structural features involving also the Trp4 indole accessibility.
Asunto(s)
Amanitinas , Espectroscopía de Resonancia Magnética/métodos , Modelos Moleculares , Conformación Proteica , Relación Estructura-Actividad , TermodinámicaRESUMEN
Three exorphins, beta-casomorphin-5, morphiceptin and its D-Pro4 analog, were studied in DMSO by means of 1H and 13C NMR spectroscopy, with the aim of detecting conformational features of potential biological significance for the mu opioid activity since the presence of two Pro residues restricts the accessible conformational space more than in all other peptides. It is found that the conformational mixtures present in solution contain relevant fractions of folded conformers, a feature that assures the observation of four different Tyr OH signals in the 500 MHz spectrum of morphiceptin. The conformer distribution of (very active) (D-Pro4)-morphiceptin is different from those of its (less active) congeners.
Asunto(s)
Endorfinas , Espectroscopía de Resonancia Magnética , Péptidos , Conformación Proteica , EstereoisomerismoRESUMEN
The structure-activity relationship of several mu selective opioid peptides has been evaluated on the basis of both experimental and theoretical approaches. The conformations of Tyr-D-Ala-Phe-Gly-NH2, the tetrapeptide N-fragment of dermorphin, and two analogues have been studied in solution by 1H NMR spectroscopy. The physicochemical environment inside the receptor has been simulated by complexing the peptides with a crown ether and dissolving the complexes in chloroform. The family of conformations derived from the NMR data possesses most of the features previously proposed for mu agonists and is fully consistent with an original model of the mu receptor based on the structures of many rigid opiates. As a simple test of this model, the synthesis of a linear peptide with significant mu activity in spite of the absence of Tyr1 is reported.