RESUMEN
Primary and secondary parasitoids of Hypogeococcus spp. (Hemiptera: Pseudococcidae) on Cactaceae, Amaranthaceae, and Portulacaceae were reported from field surveys conducted in Puerto Rico, USA, since 2009. Resident natural enemies of the Harrisia cactus mealybug (HCM), Hypogeococcus sp., which is devastating native cacti in Puerto Rico and threatening cacti in the adjacent Caribbean islands, were documented prior to the planned introductions of non-native biological control agents from South America against this pest. Two species of Encyrtidae (Hymenoptera: Chalcidoidea) were recorded as primary parasitoids of the HCM on Cactaceae: Leptomastidea hypogeococci Triapitsyn, which was the most common, and Anagyrus quilmes Triapitsyn, Logarzo & Aguirre. Another primary parasitoid, Acerophagus sp. near nubilipennis Dozier (Encyrtidae), was also reared from a different Hypogeococcus sp. on Amaranthaceae and Portulacaceae. Hyperparasitoids, usually via L. hypogeococci, included Prochiloneurus narendrani Noyes & Triapitsyn (Encyrtidae), Pachyneuron sp. (Hymenoptera: Pteromalidae), Chartocerus elongatus (Girault), and Chartocerus niger (Ashmead) (Hymenoptera: Signiphoridae). Cheiloneurus chrisiantorresi Triapitsyn sp. n. (Encyrtidae) is newly described and illustrated; its status as a suspect hyperparasitoid of Hypogeococcus sp. on Amaranthaceae and Portulacaceae, along with that of Cheiloneurus sp., will need to be verified. The recorded HCM predators include one species of Chamaemyiidae (Diptera), Leucopina bella (Loew), and three species of Coccinellidae (Coleoptera): Cryptolaemus montrouzieri Mulsant, Decadiomus austrinus (Gordon), and Scymnobius flavifrons (Melsheimer).
Asunto(s)
Dípteros/parasitología , Himenópteros/fisiología , Control Biológico de Vectores , Animales , Cactaceae , Femenino , Masculino , Puerto RicoRESUMEN
The common New World egg parasitoid of the corn leafhopper Dalbulus maidis (DeLong) (Hemiptera: Cicadellidae), an economically important pest of maize from Argentina to southern USA, has long been misidentified as the Palaearctic species Anagrus incarnatus Haliday or its synonym A. breviphragma Soyka (Hymenoptera: Mymaridae). Using a combination of genetic and morphometric methods, and available biological information, specimens reared from eggs of D. maidis in Argentina and Mexico, described and illustrated here as Anagrus (Anagrus) virlai Triapitsyn sp. n., are shown to be different from those of A. incarnatus from the Palaearctic region. Mitochondrial and nuclear ribosomal DNA sequence data provide clear evidence for the separation of the two species. Anagrus virlai is also known from Brazil, Colombia, Guadeloupe (France), and Guyana.
Asunto(s)
Himenópteros/anatomía & histología , Himenópteros/clasificación , Animales , Argentina , Brasil , Colombia , ADN Mitocondrial/genética , ADN Ribosómico/genética , Femenino , Francia , Guyana , México , Zea maysRESUMEN
The corn leafhopper, Dalbulus maidis (DeLong), is the most important leafhopper pest of maize, Zea mays, in the Americas. A survey of the diversity of its egg parasitoids was carried out in northwestern Argentina. During summer from 2004 to 2007, the samples were collected, using sentinel eggs of D. maidis on corn leaves, exposed in 48 cornfields. Sixteen species belonging to four families of Chalcidoidea (Hymenoptera) were identified. Among the parasitoid groups, Trichogrammatidae was the most represented family with eight species, followed by Mymaridae with six species. The mymarid Anagrus incarnatus Haliday and the trichogrammatid Pseudoligosita longifrangiata (Viggiani) were the most abundant and frequent parasitoids. The mean percentage of parasitism of D. maidis eggs was 16.4% and varied greatly among the sites, ranging from 0 to 56.7%; generally, it was higher in Yungas and lower in Monte province sites. The species richness was higher in the localities within the Yungas, with 13 parasitoid species, of which two species were dominant, comprising 83.6% of the collected individuals. Monte was the province that showed the highest diversity index (H´ = 1.62). In addition, we present information on the distribution, known host associations of each parasitoid species and an identification key to all species of egg parasitoids of D. maidis in Argentina.
Asunto(s)
Hemípteros/clasificación , Hemípteros/parasitología , Zea mays/parasitología , Animales , Argentina , Hemípteros/fisiología , Herbivoria , Interacciones Huésped-Parásitos , Óvulo/parasitología , Recuento de Huevos de Parásitos , Hojas de la Planta/crecimiento & desarrollo , Zea mays/crecimiento & desarrolloRESUMEN
Levels of armored scales (Hemiptera: Diaspididae) on Mexican Hass avocados imported into California over May 2008-June 2009 were monitored on 135 trucks entering the state via the Blythe border station, the entry point receiving the highest volume of fruit. Levels of live sessile scales were 3.9-fold higher than indicated in a previous survey (September 2007-April 2008) although levels of live eggs and crawlers were similar to previous levels. A survey of avocado fruit in California infested with armored scales detected four species known to be endemic but failed to find any of the seven exotic Diaspididae entering the state on Mexican fruit. Monitoring of Mexican armored scales on imported avocados from September 2007 to December 2010 recovered 10 species of parasitoids predominated by two species of Signiphora Ashmead (Hymenoptera: Signiphoridae). One of these species, Signiphora flavopalliata Ashmead, comprised 36% of all collected Mexican parasitoids and is a known hyperparasitoid. A survey of armored scale parasitoids present on commercial California avocados detected 17 genetic signatures, with only four of these in common with those detected on imported Mexican fruit. The implications of these findings are discussed.
Asunto(s)
Hemípteros/fisiología , Hemípteros/parasitología , Himenópteros/fisiología , Persea , Animales , Biodiversidad , California , Frutas , Hemípteros/clasificación , Interacciones Huésped-Parásitos , Himenópteros/clasificación , Himenópteros/crecimiento & desarrollo , Larva/clasificación , Larva/fisiología , México , Pupa/clasificación , Pupa/fisiologíaRESUMEN
We genetically characterized the prospective South American egg parasitoid candidate, Gonatocerus tuberculifemur, of the glassy-winged sharpshooter (GWSS), Homalodisca vitripennis, for a neoclassical biological control program in California. Two molecular methods, inter-simple sequence repeat-polymerase chain reaction DNA fingerprinting and a phylogeographic approach inferred from the mitochondrial cytochrome oxidase subunit I gene (COI), were utilized. Five geographic populations from South America were analyzed; in addition, a phylogenetic analysis was performed with several named and one unnamed Gonatocerus species using the COI gene. DNA fingerprinting demonstrated a fixed geographic banding pattern difference in the population from San Rafael, Mendoza Province, Argentina. The COI analysis uncovered haplotype or geographic structure in G. tuberculifemur. A neighbour-joining distance (NJ) and a single most parsimonious tree (MP) clustered the populations into two well-supported distinct clades with strong bootstrap values (97-99% and 92-99%, respectively) with populations from San Rafael clustering into clade 2 and the rest of the populations clustering into clade 1. No haplotype sharing was observed between individuals from the two clades. Phylogenetic analyses performed by NJ and MP methods with 15 Gonatocerus species confirmed species boundaries and again uncovered two distinct clades in G. tuberculifemur with strong bootstrap support (95-100% and 68-100%, respectively). However, the NJ tree supported the morphologically defined relationships better than the MP tree. The molecular evidence in the present study is suggestive of a species level divergence. Because G. tuberculifemur is under consideration as a potential biological control agent for GWSS in California, understanding cryptic variation in this species is critical.