RESUMEN
Observations on 277 children aged 3 months to 4 years with lesions of the nervous system, immunized with vaccine Act-HIB (Aventis Pasteur, France), were carried out in groups of children. The significant decrease of the level of Hib nasopharyngeal carrier state in 3.3 times and the decrease of total morbidity in acute infections of respiratory and ENT organs in 2.7 times occurred after immunization was revealed. The immunological effect of immunization on the level of protective titers against H. influenzae, type b, in children with organic CNS lesions in children was shown.
Asunto(s)
Anticuerpos Antibacterianos/sangre , Portador Sano/inmunología , Portador Sano/prevención & control , Enfermedades del Sistema Nervioso Central/inmunología , Infecciones por Haemophilus/inmunología , Infecciones por Haemophilus/prevención & control , Vacunas contra Haemophilus/administración & dosificación , Haemophilus influenzae tipo b/inmunología , Polisacáridos Bacterianos/administración & dosificación , Vacunación , Enfermedad Aguda , Cápsulas Bacterianas , Enfermedades del Sistema Nervioso Central/complicaciones , Preescolar , Infecciones por Haemophilus/complicaciones , Haemophilus influenzae tipo b/aislamiento & purificación , Humanos , Lactante , Inyecciones Intramusculares , Infecciones del Sistema Respiratorio/complicaciones , Infecciones del Sistema Respiratorio/inmunología , Infecciones del Sistema Respiratorio/prevención & control , SiberiaRESUMEN
Multiplex methylation-sensitive PCR was employed in studying the methylation of CpG islands in the RB1, p16/CDKN2A, p15/CDKN2B, p14/ARF, CDH1, MGMT, HIC1, and N33 promoter regions in breast carcinoma (105 tumors). Methylation was often observed for the two major suppressor genes involved in cell-cycle control through the Cdk-Rb-E2F signaling pathway, RB1 (18/105, 17%) and p16 (59/105, 56%); both genes were methylated in 13 tumors. Methylation involved p15 in two (2%) tumors; CDH1, in 83 (79%) tumors; MGMT, in eight (8%) tumors, and N33, in nine (9%) tumors. The p14 promoter was not methylated in the tumors examined.
Asunto(s)
Neoplasias de la Mama/genética , Metilación de ADN , Genes Supresores de Tumor , Proteínas Supresoras de Tumor , Proteínas de Ciclo Celular/genética , Islas de CpG , Inhibidor p15 de las Quinasas Dependientes de la Ciclina , Inhibidor p16 de la Quinasa Dependiente de Ciclina/genética , Femenino , Humanos , Factores de Transcripción de Tipo Kruppel , O(6)-Metilguanina-ADN Metiltransferasa/genética , Valores de Referencia , Proteína de Retinoblastoma/genética , Factores de Transcripción/genética , Proteína p14ARF Supresora de Tumor/genéticaRESUMEN
Three experimental series, in which 20 test subjects took part, were carried out to investigate the effect of super-erythemic doses of ultraviolet irradiation on metabolic parameters. It was found that UV irradiation of 12-15% of the skin area led not only to skin changes but also to functional changes in certain organs and systems, which was indicated by variations of metabolic parameters.
Asunto(s)
Metabolismo/efectos de la radiación , Rayos Ultravioleta , 11-Hidroxicorticoesteroides/metabolismo , Colesterol/metabolismo , Diuresis/efectos de la radiación , Recuento de Eritrocitos , Hematócrito , Hemoglobinas/análisis , Humanos , Recuento de Leucocitos , Metabolismo de los Lípidos , Proteínas/metabolismo , Dosis de Radiación , Piel/efectos de la radiaciónRESUMEN
From the cell-free extract of fodder yeast Candida tropicalis NADP-specific glutamate dehydrogenase was isolated and partially purified (75-fold) by means of fractional precipitation by ammonium sulphate and ion-exchange chromatography on DEAE-cellulose. The preparation was investigated with the aid of polyacrylamide gel electrophoresis. Kinetic characteristics of the enzyme in the cell-free extract and partially purified preparation were derived.