RESUMEN
Escherichia coli, an Enterobacterales member, is a normal representative of the microbiota of homeothermic animals. Most strains are commensal, but several pathotypes can cause disease, and numerous antimicrobial resistance factors have been identified. These bacteria have spread rapidly in recent years, highlighting the importance of screening the environment and non-human reservoirs for virulent strains and/or those presenting resistance factors, in addition to other microorganisms of public health importance. In this context, this study aimed to survey Enterobacteriales present in seabirds sampled along the Brazilian coast, comparing findings between migratory and resident birds, as well as between wrecked and non-wrecked animals. Escherichia coli pathotypes were also characterized through rapid seroagglutination and polymerase chain reaction techniques and antimicrobial resistance profiles were investigated through the disc agar diffusion method. Cloacal, ocular, oral, tracheal, and skin lesion swabs, as well as fresh feces, were collected from 122 seabirds. The findings indicate these animals as important hosts for opportunistic human pathogens. Escherichia coli strains were identified in 70 % of the analyzed seabirds, 62 % of which displaying resistant or intermediate profiles to at least one antimicrobial, while 7% were multiresistant. Resistance to tetracycline (22 %), nalidixic acid (15 %), trimethoprim-sulfamethozaxol (14 %) and ampicillin (12 %) were the most prevalent. Resistance to cefoxitin, a critically important antimicrobial for human medicine, was also detected. Virulence genes for one of the EAEC, ETEC or EPEC pathotypes were detected in 30 % of the identified strains, the first two described in seabirds for the first time. The EAEC gene was detected in 25 % of the sampled seabirds, all resident, 8 % of which exhibited a multidrug-resistant profile. Thus, seabirds comprise important reservoirs for this pathotype. Escherichia coli was proven an ubiquitous and well-distributed bacterium, present in all evaluated bird species and sampling sites (except Marajó Island). According to the chi-square test, no significant differences between E. coli prevalences or antimicrobial resistance profiles between migratory and resident and between wrecked and non-wrecked seabirds were observed. Thus, migratory birds do not seem to contribute significantly to E. coli frequencies, pathotypes or antimicrobial resistance rates on the Brazilian coast.
RESUMEN
In view of growing concerns, in a One Health context, regarding the transport and dissemination of pathogenic microorganisms among seabirds and other vertebrate animals, including humans, the aim of this study was to identify Salmonella spp. in stranded and non-stranded resident and migratory wild seabirds from the Brazilian coast. Antimicrobial susceptibility and molecular profiles, quinolone resistance genes and antigenic characterization of the isolates were also carried out. Fresh faeces and cloacal swabs were obtained totaling 122 seabirds sampled throughout different Brazilian coast regions. At the laboratory, sample culturing, Salmonella spp. isolation and biochemical identification were performed, followed by antigenic profile identification by serum agglutination, susceptibility profile characterization by the agar disc diffusion technique, detection of quinolone resistance genes (qnrA, qnrB, qnrS) using the multiplex polymerase chain reaction technique (multiplex PCR) and, finally, isolates profiles identification by pulsed field gel electrophoresis (PFGE). Salmonella enterica subsp. enterica was identified in 7% of the studied birds, comprising three different serovars: Panama (63 %), Typhimurium (25 %) and Newport (13 %). The most important findings reported herein are the first description of Salmonella panama in seabirds and the totality of isolates being resistant (or intermediate) to at least one tested antimicrobial, with emphasis on quinolone resistance. The molecular results suggest that the observed resistance cannot be explained by the presence of plasmid-mediated quinolone resistance genes. The PFGE suggests that the Panama and Newport profiles detected herein are not yet widespread in Brazil, unlike Typhimurium, which is already well distributed throughout the country. Considering this finding, we suggest that seabirds are an important link in the epidemiological chain of this serovar. The monitoring of these bacteria in seabirds, as well as of their susceptibility profiles to antimicrobials, must be continuous, strengthening the role of these animals as environmental health indicators and sentinels.
Asunto(s)
Aves/microbiología , Farmacorresistencia Bacteriana , Farmacorresistencia Bacteriana Múltiple , Salmonella , Animales , Antibacterianos/farmacología , Brasil/epidemiología , Farmacorresistencia Bacteriana/efectos de los fármacos , Electroforesis en Gel de Campo Pulsado/veterinaria , Lindera/microbiología , Pruebas de Sensibilidad Microbiana/veterinaria , Salmonella/clasificación , Salmonella/efectos de los fármacos , Salmonella enterica , Salmonella typhimuriumRESUMEN
BACKGROUND: Bovine vaccinia is an exanthematic disease caused by Vaccinia virus (VACV). This zoonosis has been associated with several cases of bovine infection, particularly in milk herds. Farmers, milkers and their close contacts developed lesions on the hands, forearms, legs and face accompanied by fever, headache, malaise, myalgia and axillary, inguinal and cervical lymphadenopathy. VACV infections have a significant public health impact due to their occupational character, high frequency of transmission and the improper medical treatment often applied. OBJECTIVES: To study natural human infection by VACV and to analyze clinical and epidemiological aspects, emphasizing the patients' immunological status. STUDY DESIGN: Ninety-eight individuals from rural properties with bovine vaccinia (BV) outbreaks who were at risk due to contact were submitted to epidemiological and clinical studies. From these individuals, 54 sera were analyzed by serological and molecular procedures. This study was conducted in Rio de Janeiro State from September 2002 to October 2006. RESULTS: The clinical frequency of infection was 52.0%, with 57.4% ELISA and 43.0% PRNT-positive reactions. DNAemia was detected in 18.5% of the analyzed sera, and 50% of smallpox-vaccinated individuals developed symptoms. CONCLUSIONS: This study confirms the high clinical frequency of human VACV infection, even among vaccinated individuals. The infection was related to detection of IgG- or IgM-specific antibodies that correlates in most of the cases with positive PRNT. The DNAemia suggests viremia during VACV natural infections. Our data indicate that patients vaccinated against smallpox may no longer be protected.
Asunto(s)
Enfermedades de los Bovinos/epidemiología , Enfermedades de los Bovinos/transmisión , Brotes de Enfermedades , Virus Vaccinia/aislamiento & purificación , Vaccinia/veterinaria , Zoonosis/epidemiología , Zoonosis/transmisión , Adolescente , Adulto , Animales , Anticuerpos Antivirales/sangre , Brasil , Bovinos , Enfermedades de los Bovinos/virología , ADN Viral/sangre , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Persona de Mediana Edad , Población Rural , Vaccinia/epidemiología , Vaccinia/transmisión , Adulto Joven , Zoonosis/virologíaRESUMEN
O trabalho consistiu no estudo epidemiológico do surto de poxvirose bovina no Estado do Espírito Santo no período de 2002 a 2005. A coleta dos dados deu-se em 28 propriedades de gado bovino leiteiro de 08 municípios do Sul do Estado do Espírito Santo com casos de doenças pústulo-vesicular em bovinos, no período de agosto de 2002 a maio de 2005. Observou-se que a prevalência da doença no gado bovino leiteiro variou de 10,2% a 100% das propriedades com média de 52,7%. Através de isolamento viral e PCR do gene Timidina Quinase (TK), identificou-se o vírus do gênero Orthopoxvirus como o agente etiológico da poxvirose bovina. Em todas as propriedades constatou-se uma perda econômica, principalmente relacionada à queda na produção leiteira. A poxvirose bovina assumiu um caráter epidêmico no Sul do Estado do Espírito Santo, com grande impacto econômico e importância em saúde pública, como uma zoonose ocupacional entre os ordenhadores. Baseadas nos dados obtidos, medidas de cunho fiscal-sanitário e de orientação ao produtor agropecuário foram implementadas, visando conter a disseminação da doença entres as propriedades, e os outros municípios do Estado.
The purpose of this study was to investigate the epidemiological data of bovine poxviruses in the State of Espírito Santo during the years 2002 to 2005. The sample consisted of 28 properties that produce cow's milk in 08 cities from the South of the State, in wich were present cases of bonive pustulo-vesicular disease between 2002 august to 2005 may. The bovine disease was found in 10,2% to 100% of the properties with a mean of 52,7%. Throughout virus isolation techniques and PCR of the Timidin Kinase gene, an Orthopoxvirus was identified as the etiological agent of the bovine yiruses. All the properties reported economic losts related to a decrease in milk production. The bovine poxviruses assumed an epidemic feature in the South of the State of Espírito Santo promoting a big economic impact and representing an important public health worry as an occupational zoonosis mainly for the milkers. Thus, based on the obtained data, policy and sanitary statements, and educational strategies, were applied in the properties in order to stop the disease dissemination over the neighbor cities.
Asunto(s)
Bovinos , Infecciones por Poxviridae/epidemiología , Orthopoxvirus/aislamiento & purificación , Parapoxvirus/aislamiento & purificaciónRESUMEN
O trabalho consistiu no estudo epidemiológico do surto de poxvirose bovina no Estado do Espírito Santo no período de 2002 a 2005. A coleta dos dados deu-se em 28 propriedades de gado bovino leiteiro de 08 municípios do Sul do Estado do Espírito Santo com casos de doenças pústulo-vesicular em bovinos, no período de agosto de 2002 a maio de 2005. Observou-se que a prevalência da doença no gado bovino leiteiro variou de 10,2% a 100% das propriedades com média de 52,7%. Através de isolamento viral e PCR do gene Timidina Quinase (TK), identificou-se o vírus do gênero Orthopoxvirus como o agente etiológico da poxvirose bovina. Em todas as propriedades constatou-se uma perda econômica, principalmente relacionada à queda na produção leiteira. A poxvirose bovina assumiu um caráter epidêmico no Sul do Estado do Espírito Santo, com grande impacto econômico e importância em saúde pública, como uma zoonose ocupacional entre os ordenhadores. Baseadas nos dados obtidos, medidas de cunho fiscal-sanitário e de orientação ao produtor agropecuário foram implementadas, visando conter a disseminação da doença entres as propriedades, e os outros municípios do Estado.(AU)
The purpose of this study was to investigate the epidemiological data of bovine poxviruses in the State of Espírito Santo during the years 2002 to 2005. The sample consisted of 28 properties that produce cow's milk in 08 cities from the South of the State, in wich were present cases of bonive pustulo-vesicular disease between 2002 august to 2005 may. The bovine disease was found in 10,2% to 100% of the properties with a mean of 52,7%. Throughout virus isolation techniques and PCR of the Timidin Kinase gene, an Orthopoxvirus was identified as the etiological agent of the bovine yiruses. All the properties reported economic losts related to a decrease in milk production. The bovine poxviruses assumed an epidemic feature in the South of the State of Espírito Santo promoting a big economic impact and representing an important public health worry as an occupational zoonosis mainly for the milkers. Thus, based on the obtained data, policy and sanitary statements, and educational strategies, were applied in the properties in order to stop the disease dissemination over the neighbor cities.(AU)
Asunto(s)
Parapoxvirus/aislamiento & purificación , Orthopoxvirus/aislamiento & purificación , Infecciones por Poxviridae/epidemiología , BovinosRESUMEN
No presente trabalho foram utilizados 54 bovinos, mestiços, portadores da papilomatose plana e/ou pedunculada, de ambos os sexos e com idade variando entre 1 e 7 anos. Os animais foram divididos aleatoriamente em três grupos, com 18 bovinos cada. O Grupo 1 não recebeu nenhum tipo de tratamento (grupo controle). O Grupo 2 foi constituído pelos animais tratados homeopaticamente. O Grupo 3 foi constituído pelos animais que receberam tratamento fitoterápico. Os resultados obtidos demostraram que os tratamentos homeopático e fitoterápico foram eficazes na cura da papilomatose cutânea bovina.