RESUMEN
Biodegradable membranes for cartilage applications were manufactured starting from polymeric networks of a lactose-modified chitosan (CTL), previously proposed for chondrocytes stimulation. This implantable biomaterial was conceived as a reservoir of a bioactive polymer that could promote the activity of chondrocytes and the healing of cartilage defects. Freeze-drying of reticulated hydrogels enabled to obtain pliable membranes with a homogeneous polymeric texture, as pointed out by scanning electron microscopy analyses. Swelling tests and dimensional evaluations showed that the material is able to absorb physiological fluids and expand gradually upon rehydration. This feature was evaluated on a simulated cartilage defect on pig's humerus (ex vivo), which revealed the capability of the membranes to progressively fit the tissue voids on the damaged cartilage. The rheological properties of the rehydrated membranes pointed out their peculiar strain-stiffening behavior, which represents a promising feature for the regeneration of tissues subjected to variable mechanical loads and deformations. Biological in vitro studies demonstrated the biocompatibility of the membranes in contact with primary chondrocytes and osteoblasts. Taken together, these results represent a starting point for the development of a novel generation of implantable biomaterials for cartilage treatment based on CTL.
Asunto(s)
Implantes Absorbibles , Materiales Biocompatibles/química , Cartílago Articular/lesiones , Quitosano/análogos & derivados , Condrocitos/citología , Animales , Células Cultivadas , PorcinosRESUMEN
Chitosan-based films with incorporated supercritical CO2 hop extract (HE) were developed and evaluated regarding structural, physicochemical, and antibacterial properties. The morphological and spectroscopic analyses have confirmed successful incorporation of HE into the polymer matrix, which affected films' structure and visual appearance. The presence of HE has caused a reduction in the hydrophilic character of films, but also provided a complete UV light blockage at wavelengths below 350 nm. Furthermore, a declining trend of tensile strength (from 14.4 MPa to 6.4 MPa) and Young's modulus (from 218.8 MPa to 26.9 MPa), as well as an ascending trend of elongation at break (from 10.7% to 35.1%), have been observed after the extract incorporation. The total phenolic content in the films was up to â¼13 mgGAE gfilm-1. Besides, the HE-loaded films exhibited antibacterial activity against foodborne pathogen Bacillus subtilis.
Asunto(s)
Antibacterianos , Bacillus subtilis/efectos de los fármacos , Materiales Biocompatibles , Quitosano , Extractos Vegetales , Antibacterianos/química , Antibacterianos/farmacología , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Dióxido de Carbono/química , Quitosano/química , Quitosano/farmacología , Módulo de Elasticidad , Interacciones Hidrofóbicas e Hidrofílicas , Extractos Vegetales/química , Extractos Vegetales/farmacología , Resistencia a la TracciónRESUMEN
Implantable membranes based on alginate and hyaluronic acid (HA) were manufactured to obtain a rapidly resorbing pliable mesh for the in situ administration of HA to intestinal tissue. Morphological analyses of this interpenetrated matrix pointed out a homogeneous polymeric texture while degradation studies demonstrated that the material is able to dissolve in physiological solutions within few days. Biological studies in vitro showed that the membrane is biocompatible towards human dermal fibroblasts and that liquid extracts from the HA-containing membrane can stimulate wound healing. A preliminary in vivo biocompatibility study on rats showed that the membranes in direct contact with the intestine did not elicit any acute adverse reaction or immune response, while only a mild inflammatory reaction was noticed at the mesenteric or serosal region. Overall, these results appear to support the application of these polysaccharide-based materials for intestinal wound healing.
Asunto(s)
Materiales Biocompatibles/química , Ácido Hialurónico/química , Herida Quirúrgica/terapia , Cicatrización de Heridas , Alginatos/química , Animales , Supervivencia Celular , Fibroblastos/metabolismo , Ácidos Hexurónicos/química , Humanos , Inflamación , Ensayo de Materiales , Ratones , Células 3T3 NIH , Polímeros , Polisacáridos/química , Ratas , Piel/metabolismoRESUMEN
Developing synthetic materials able to mimic micro- and macrorheological properties of natural networks opens up to novel applications and concepts in materials science. The present contribution describes an active network based on a semi-synthetic polymer, a lactitol-bearing chitosan derivative (Chitlac), and a transient inorganic cross-linker, boric acid. Due to the many and diverse anchoring points for boric acid on the flanking groups of Chitlac, the cross-links constantly break and reform in a highly dynamic fashion. The consequence is a network with unusual non-equilibrium and mechanical properties closely resembling the rheological behavior of natural three-dimensional arrangements and of cytoskeleton. Concepts like network nucleation, reorganization and disassembly are declined in terms of amount of the cross-linker, which acts as a putative motor for remodeling of the network upon application of energy. The out-of-equilibrium and non-linear behavior render the semi-synthetic system of great interest for tissue engineering and for developing in-vitro mimics of natural active matrices.
Asunto(s)
Materiales Biocompatibles/química , Quitosano/química , Lactosa/química , Ácidos Bóricos/química , Dispersión Dinámica de Luz , ReologíaRESUMEN
Current strategies in Central Nervous System (CNS) repair focus on the engineering of artificial scaffolds for guiding and promoting neuronal tissue regrowth. Ideally, one should combine such synthetic structures with stem cell therapies, encapsulating progenitor cells and instructing their differentiation and growth. We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons. Moreover, we investigated the feasibility of combining these approaches with engineered mesenchymal stem cells able to release neurotrophic factors. We show here that composite bio-constructs made of Chitlac, a Chitosan derivative, favor hippocampal neuronal growth, synapse formation and the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins. STATEMENT OF SIGNIFICANCE: In our work, we characterized polysaccharide-based bioactive platforms as biocompatible materials for nerve tissue engineering. We show that Chitlac-thick substrates are able to promote neuronal growth, differentiation, maturation and formation of active synapses. These observations support this new material as a promising candidate for the development of complex bio-constructs promoting central nervous system regeneration. Our novel findings sustain the exploitation of polysaccharide-based scaffolds able to favour neuronal network reconstruction. Our study shows that Chitlac-thick may be an ideal candidate for the design of biomaterial scaffolds enriched with stem cell therapies as an innovative approach for central nervous system repair.
Asunto(s)
Neuronas/citología , Neuronas/efectos de los fármacos , Polisacáridos/química , Células Madre/citología , Ingeniería de Tejidos/métodos , Animales , Materiales Biocompatibles , Técnicas de Cultivo de Célula , Diferenciación Celular , Células Cultivadas , Quitosano/química , Femenino , Vidrio , Hipocampo/citología , Hidrogeles , Microscopía de Fuerza Atómica , Microscopía Confocal , Neuronas Motoras/citología , Neuronas Motoras/metabolismo , Factores de Crecimiento Nervioso , Regeneración Nerviosa , Neurogénesis , Técnicas de Placa-Clamp , Fenotipo , Polímeros/química , Porosidad , Ratas , Electricidad Estática , Andamios del Tejido/químicaRESUMEN
Chronic non-healing wounds are a clinically important problem in terms of number of patients and costs. Wound dressings such as hydrogels, hydrocolloids, polyurethane films and foams are commonly used to manage these wounds since they tend to maintain a moist environment which is shown to accelerate re-epithelialization. The use of antibacterial compounds is important in the management of wound infections. A novel wound-dressing material based on a blended matrix of the polysaccharides alginate, hyaluronic acid and Chitlac-silver nanoparticles is here proposed and its application for wound healing is examined. The manufacturing approach to obtain membranes is based on gelling, foaming and freeze-casting of alginate, hyaluronic acid and Chitlac-silver nanoparticles mixtures using calcium ions as the cross-linking agent. Comprehensive evaluations of the morphology, swelling kinetics, permeability, mechanical characteristics, cytotoxicity, capability to inhibit metalloproteinases and of antibacterial property were conducted. Biological in vitro studies demonstrated that hyaluronic acid released by the membrane is able to stimulate the wound healing meanwhile the metal silver exploits an efficient antibacterial activity against both planktonic bacteria and biofilms. Overall, the experimental data evidence that the studied material could be used as antibacterial wound dressing for wound healing promotion.
Asunto(s)
Alginatos/química , Vendajes , Ácido Hialurónico/administración & dosificación , Nanopartículas del Metal/administración & dosificación , Plata/administración & dosificación , Cicatrización de Heridas/efectos de los fármacos , Infección de Heridas/prevención & control , Antibacterianos/administración & dosificación , Células Cultivadas , Portadores de Fármacos/química , Portadores de Fármacos/uso terapéutico , Fibroblastos/efectos de los fármacos , Fibroblastos/fisiología , Ácido Glucurónico/química , Ácidos Hexurónicos/química , Humanos , Queratinocitos/efectos de los fármacos , Queratinocitos/fisiología , Ensayo de Materiales , Pruebas de Sensibilidad Microbiana , Heridas y Lesiones/microbiología , Heridas y Lesiones/patología , Heridas y Lesiones/terapiaRESUMEN
Injectable bone fillers represent an attractive strategy for the treatment of bone defects. These injectable materials should be biocompatible, capable of supporting cell growth and possibly able to exert antibacterial effects. In this work, nanocomposite microbeads based on alginate, chitlac, hydroxyapatite and silver nanoparticles were prepared and characterized. The dried microbeads displayed a rapid swelling in contact with simulated body fluid and maintained their integrity for more than 30â days. The evaluation of silver leakage from the microbeads showed that the antibacterial metal is slowly released in saline solution, with less than 6% of silver released after 1â week. Antibacterial tests proved that the microbeads displayed bactericidal effects toward Staphylococcus aureus, Pseudomonas aeruginosa and Staphylococcus epidermidis, and were also able to damage pre-formed bacterial biofilms. On the other hand, the microbeads did not exert any cytotoxic effect towards osteoblast-like cells. After characterization of the microbeads bioactivity, a possible means to embed them in a fluid medium was explored in order to obtain an injectable paste. Upon suspension of the particles in alginate solution or alginate/hyaluronic acid mixtures, a homogenous and time-stable paste was obtained. Mechanical tests enabled to quantify the extrusion forces from surgical syringes, pointing out the proper injectability of the material. This novel antibacterial bone filler appears as a promising material for the treatment of bone defects, in particular when possible infections could compromise the bone-healing process. Copyright © 2016 John Wiley & Sons, Ltd.
Asunto(s)
Antibacterianos/farmacología , Huesos/efectos de los fármacos , Nanopartículas del Metal/química , Nanocompuestos/química , Polisacáridos/farmacología , Plata/farmacología , Biopelículas/efectos de los fármacos , Muerte Celular/efectos de los fármacos , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Humanos , Pruebas de Sensibilidad Microbiana , Microesferas , Nanocompuestos/ultraestructura , Pseudomonas aeruginosa/efectos de los fármacos , Pseudomonas aeruginosa/crecimiento & desarrollo , Staphylococcus epidermidis/efectos de los fármacos , Staphylococcus epidermidis/crecimiento & desarrolloRESUMEN
The effect of transient cross-links has been explored on a lactose-modified chitosan, which previously had shown interesting biological features. The presence of galactose side chains and of the polyol spacer resulted particularly appealing for the reticulation by borate ions. The interaction between chitlac and borax was investigated by means of 11B NMR while rheology pointed to a marked non-linear behavior depending on the amount of borax added to the system. The presence of limited amount of cross-linking ion led to dilatant behavior when the steady flow curve was measured. In addition, strain stiffening was noticed on elastic response upon exceeding a critical stress, indicating a transient nature in the formation of the cross-links. The non-linear response of chitlac in the presence of borax compared surprisingly well with the one showed by proteins composing the natural ECM pointing at a possible role of mechanotransduction in the biological significance of the modified chitosan.
Asunto(s)
Materiales Biocompatibles/química , Materiales Biomiméticos/química , Boratos/química , Quitosano/química , Reactivos de Enlaces Cruzados/química , Lactosa/química , Elasticidad , Humanos , Espectroscopía de Resonancia Magnética , Reología , Estrés Mecánico , ViscosidadRESUMEN
The present paper explores the effect of boric acid on Chitlac, a lactose-modified chitosan which had previously shown interesting biological and physical-chemical features. The herewith-reported experimental evidences demonstrated that boric acid binds to Chitlac, producing conformational and association effects on the chitosan derivative. The thermodynamics of boric acid binding to Chitlac was explored by means of 11B NMR, circular dichroism (CD), and UV-vis spectroscopy, while macromolecular effects were investigated by means of viscometry and dynamic light scattering (DLS). The experimental results revealed a chain-chain association when limited amounts of boric acid were added to Chitlac. However, upon exceeding a critical boric acid limit dependent on the polysaccharide concentration, the soluble aggregates disentangle. The rheological behavior of Chitlac upon treatment with boric acid was explored showing a dilatant behavior in conditions of steady flow. An uncommonly high dependence in the scaling law between the zero-shear viscosity and the concentration of Chitlac was found, i.e., η0 â CCTL5.8, pointing to interesting potential implications of the present system in biomaterials development.
Asunto(s)
Ácidos Bóricos/química , Quitosano/química , Lactosa/química , Materiales Biocompatibles/química , Sustancias Macromoleculares/química , Espectroscopía de Resonancia Magnética , Polisacáridos/química , ViscosidadRESUMEN
Adhesive coatings for implantable biomaterials can be designed to prevent material displacement from the site of implant. In this paper, a strategy based on the use of melanin-like nanoparticles (MNPs) for the development of adhesive coatings for polysaccharidic membranes was devised. MNPs were synthesized in vitro and characterized in terms of dimensions and surface potential, as a function of pH and ionic strength. The in vitro biocompatibility of MNPs was investigated on fibroblast cells, while the antimicrobial properties of MNPs in suspension were evaluated on E. coli and S. aureus cultures. The manufacturing of the adhesive coatings was carried out by spreading MNPs over the surface of polysaccharidic membranes; the adhesive properties of the nano-engineered coating to the target tissue (intestinal serosa) were studied in simulated physiological conditions. Overall, this study opens for novel approaches in the design of naturally inspired nanostructured adhesive systems.
Asunto(s)
Adhesivos/química , Materiales Biocompatibles Revestidos/química , Melaninas/química , Nanopartículas/química , Adulto , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Materiales Biocompatibles Revestidos/síntesis química , Materiales Biocompatibles Revestidos/farmacología , Escherichia coli/efectos de los fármacos , Escherichia coli/crecimiento & desarrollo , Escherichia coli/ultraestructura , Fibroblastos/efectos de los fármacos , Fibroblastos/metabolismo , Humanos , Membranas/efectos de los fármacos , Membranas/metabolismo , Membranas/ultraestructura , Microscopía Electrónica de Rastreo , Nanopartículas/ultraestructura , Polisacáridos/química , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/crecimiento & desarrollo , Staphylococcus aureus/ultraestructuraRESUMEN
All-organic porous sponges were obtained throughout the direct and solvent-free (oven 105 °C, time>6â h) crosslinking of TEMPO-oxidized cellulose nanofibers (TOCNF) with 25â kDa branched polyethyleneimine (bPEI) in the presence of different amounts of citric acid (CA) as co-crosslinker. The chemical and mechanical stability of these materials was provided by the formation of amide bonds between the carboxylic moieties of TOCNF and CA with the primary amines of bPEI. The mechanical properties were investigated under static and dynamic loads with both dry and wet samples. The materials had the interesting capability to recover their shape with reduced losses in mechanical resistance, while their Young's modulus progressively increased with the content of CA. In work toward developing possible applications of bPEI-TOCNF sponges in drug delivery, amoxicillin (AM) and ibuprofen (IB) were considered as model drugs. All materials showed very good performance in adsorbing both AM and IB (ca. 200â mg g-1 ) from methanol solution. In particular, an increased adsorption of IB was observed in parallel to the increase of citrate moieties in the samples. Moreover, samples crosslinked in presence of CA showed slower kinetic release in aqueous environments than materials obtained without CA.
RESUMEN
The manuscript focuses on the demixing of hyaluronan and alginate in the hydrogel state. Binary solutions of the two polysaccharides have been treated with Ca2+ as the alginate cross-linking ion and the radial distribution of the two components in the hydrogels was measured by means of 1H NMR. These results revealed the presence of alginate-enriched and hyaluronan-enriched domains stemming from a polysaccharide demixing. The hydrogels were characterized by means of uniaxial compression and creep-compliance measurements which showed that the demixing increased the overall resistance of the hydrogel to stress. In addition, due to the viscoelastic properties of hyaluronan, a marked increase of the Newtonian viscosity of the constructs was noticed. The peculiarity of the effect of hyaluronan was demonstrated by the use of an alginate unable to form gel by binding non-calcium binding alginate, i.e. mannuronan, ruling out the effect of viscosity over the time-dependent behavior of the mixed hyaluronan-alginate hydrogels.
Asunto(s)
Alginatos/química , Ácido Hialurónico/química , Materiales Biocompatibles/química , Geles , Ácido Glucurónico/química , Ácidos Hexurónicos/química , Fenómenos MecánicosRESUMEN
Silver-based products have been proven to be effective in retarding and preventing bacterial growth since ancient times. In the field of restorative dentistry, the use of silver ions/nanoparticles has been explored to counteract bacterial infections, as silver can destroy bacterial cell walls by reacting with membrane proteins. However, it is also cytotoxic towards eukaryotic cells, which are capable of internalizing nanoparticles. In this work, we investigated the biological effects of Chitlac-nAg, a colloidal system based on a modified chitosan (Chitlac), administered for 24-48 h to a co-culture of primary human gingival fibroblasts and Streptococcus mitis in the presence of saliva, developed to mimic the microenvironment of the oral cavity. We sought to determine its efficiency to combat oral hygiene-related diseases without affecting eukaryotic cells. Cytotoxicity, reactive oxygen species production, apoptosis induction, nanoparticles uptake, and lysosome and autophagosome metabolism were evaluated. In vitro results show that Chitlac-nAg does not exert cytotoxic effects on human gingival fibroblasts, which seem to survive through a homoeostasis mechanism involving autophagy. That suggests that the novel biomaterial Chitlac-nAg could be a promising tool in the field of dentistry.
Asunto(s)
Autofagia , Técnicas de Cocultivo , Fibroblastos/microbiología , Adhesión Bacteriana/efectos de los fármacos , Supervivencia Celular , Quitosano/farmacología , Coloides/química , Materiales Dentales , Fibroblastos/citología , Citometría de Flujo , Encía/citología , Humanos , Iones , L-Lactato Deshidrogenasa/química , Nanopartículas del Metal/química , Microscopía Electrónica de Transmisión , Especies Reactivas de Oxígeno/química , Plata/farmacología , Streptococcus mitis/efectos de los fármacosRESUMEN
Alginate-based membranes containing hyaluronic acid (HA) were manufactured by freeze-drying calcium-reticulated hydrogels. The study of the distribution of the two macromolecules within the hydrogel enabled to highlight a polymer demixing mechanism that tends to segregate HA in the external parts of the constructs. Resistance and pliability of the membranes were tuned, while release and degradation studies enabled to quantify the diffusion of both polysaccharides in physiological solution and to measure the viable lifetime of the membranes. Biological studies in vitro proved that the liquid extracts from the HA-containing membranes stimulate wound healing and that fibroblasts are able to colonize the membranes. Overall, such novel alginate-HA membranes represent a promising solution for several medical needs, in particular for wound treatment, giving the possibility to provide an in situ administration of HA from a resorbable device.
Asunto(s)
Portadores de Fármacos/química , Ácido Hialurónico/química , Hidrogeles/química , Polisacáridos/química , Adulto , Portadores de Fármacos/farmacología , Liberación de Fármacos , Humanos , Ensayo de Materiales , Fenómenos Mecánicos , Membranas Artificiales , Polisacáridos/farmacología , Reología , Piel/efectos de los fármacos , Cicatrización de Heridas/efectos de los fármacosRESUMEN
The main functions of biological adhesives and sealants are to repair injured tissues, reinforce surgical wounds, or even replace common suturing techniques. In general surgery, adhesives must match several requirements taking into account clinical needs, biological effects, and material features; these requirements can be fulfilled by specific polymers. Natural or synthetic polymeric materials can be employed to generate three-dimensional networks that physically or chemically bind to the target tissues and act as hemostats, sealants, or adhesives. Among them, fibrin, gelatin, dextran, chitosan, cyanoacrylates, polyethylene glycol, and polyurethanes are the most important components of these interfaces; various aspects regarding their adhesion mechanisms, mechanical performance, and resistance to body fluids should be taken into account to choose the most suitable formulation for the target application. This review aims to describe the main adhesives and sealant materials for general surgery applications developed in the past decades and to highlight the most important aspects for the development of future formulations.
Asunto(s)
Adhesivo de Tejido de Fibrina , Polímeros , Procedimientos Quirúrgicos Operativos/métodos , Adhesivos Tisulares , Animales , Adhesivo de Tejido de Fibrina/química , Adhesivo de Tejido de Fibrina/uso terapéutico , Humanos , Polímeros/química , Polímeros/uso terapéutico , Adhesivos Tisulares/química , Adhesivos Tisulares/uso terapéuticoRESUMEN
The present manuscript addresses the description of binary systems of hyaluronan (HA) and alginate (Alg) in semi-concentrated solution. The two polysaccharides were completely miscible in the entire range of relative weight fraction explored at a total polymer concentration of up to 3% (w/V). The rheological study encompassed steady flow and mechanical spectra for HA/Alg systems at different weight fractions with hyaluronan at different molecular weights. These extensive analyses allowed us to propose a model for the molecular arrangement in solution that envisages a mutual exclusion between the two polysaccharides even though a clear phase separation does not occur. This result may have profound implications when combinations of alginate and hyaluronan are proposed in the field of biomedical materials.
Asunto(s)
Alginatos/química , Ácido Hialurónico/química , Reología , Ácido Glucurónico/química , Ácidos Hexurónicos/química , Soluciones , ViscosidadRESUMEN
Treatment of non-healing wounds represents hitherto a severe dilemma because of their failure to heal caused by repeated tissue insults, bacteria contamination and altered physiological condition. This leads to face huge costs for the healthcare worldwide. To this end, the development of innovative biomaterials capable of preventing bacterial infection, of draining exudates and of favoring wound healing is very challenging. In this study, we exploit a novel technique based on the slow diffusion of tripolyphosphate for the preparation of macroscopic chitosan hydrogels to obtain soft pliable membranes which include antimicrobial silver nanoparticles (AgNPs) stabilized by a lactose-modified chitosan (Chitlac). UV-Vis and TEM analyses demonstrated the time stability and the uniform distribution of AgNPs in the gelling mixture, while swelling studies indicated the hydrophilic behavior of membrane. A thorough investigation on bactericidal properties of the material pointed out the synergistic activity of chitosan and AgNPs to reduce the growth of S. aureus, E. coli, S. epidermidis, P. aeruginosa strains and to break apart mature biofilms. Finally, biocompatibility assays on keratinocytes and fibroblasts did not prove any harmful effects on the viability of cells. This novel technique enables the production of bioactive membranes with great potential for the treatment of non-healing wounds.
Asunto(s)
Antibacterianos/administración & dosificación , Quitosano/química , Hidrogeles , Membranas Artificiales , Polifosfatos/química , Plata , Heridas y Lesiones/tratamiento farmacológico , Antibacterianos/uso terapéutico , Materiales BiocompatiblesRESUMEN
Biostable fiber-reinforced composites, based on bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix reinforced with E-glass fibers have been successfully used in cranial reconstructions and the material has been approved for clinical use. As a further refinement of these implants, antimicrobial, non-cytotoxic coatings on the composites were created by an immersion procedure driven by strong electrostatic interactions. Silver nanoparticles (nAg) were immobilized in lactose-modified chitosan (Chitlac) to prepare the bacteriostatic coatings. Herein, we report the use of inkjet technology (a drop-on-demand inkjet printer) to deposit functional Chitlac-nAg coatings on the thermoset substrates. Characterization methods included scanning electron microscopy, scanning white light interferometry and electro-thermal atomic absorption spectroscopy. Inkjet printing enabled the fast and flexible functionalization of the thermoset surfaces with controlled coating patterns. The coatings were not impaired by the printing process: the kinetics of silver release from the coatings created by inkjet printing and conventional immersion technique was similar. Further research is foreseen to optimize printing parameters and to tailor the characteristics of the coatings for specific clinical applications.
Asunto(s)
Bioimpresión/métodos , Quitosano/química , Materiales Biocompatibles Revestidos/química , Lactosa/química , Nanocompuestos/química , Plata/química , Biotecnología/métodos , Sustitutos de Huesos , Prótesis e ImplantesRESUMEN
Polysaccharide networks, in the form of hydrogels and dried membranes based on chitosan and on the cross-linker tripolyphosphate (TPP), were developed using a novel approach. TPP was incorporated into chitosan by slow diffusion to favor a controlled gelation. By varying chitosan, TPP, and NaCl concentration, transition from inhomogeneous to homogeneous systems was achieved. Rheology and uniaxial compression tests enabled to identify the best performing hydrogel composition with respect to mechanical properties. FTIR, (31)P NMR, and spectrophotometric methods were used to investigate the interaction chitosan-TPP, the kinetics of phosphates diffusion during the dialysis and the amount of TPP in the hydrogel. A freeze-drying procedure enabled the preparation of soft pliable membranes. The lactate dehydrogenase assay demonstrated the biocompatibility of the membranes toward fibroblasts. Overall, we devised a novel approach to prepare homogeneous macroscopic chitosan/TPP-based biomaterials with tunable mechanical properties and good biocompatibility that show good potential as novel polysaccharide derivatives.
Asunto(s)
Materiales Biocompatibles , Quitosano , Fibroblastos/enzimología , Hidroliasas/metabolismo , Hidrogeles , Polifosfatos , Polisacáridos , Animales , Materiales Biocompatibles/síntesis química , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Quitosano/química , Quitosano/farmacología , Fibroblastos/citología , Hidrogeles/síntesis química , Hidrogeles/química , Hidrogeles/farmacología , Ensayo de Materiales , Ratones , Células 3T3 NIH , Polifosfatos/química , Polifosfatos/farmacología , Polisacáridos/química , Polisacáridos/farmacologíaRESUMEN
This study sought to evaluate the in vitro biological response of human gingival fibroblasts (HGFs) co-coltured with Streptococcus mitis to bisphenol A glycidylmethacrylate/triethylene glycol dimethacrylate (BisGMA/TEGDMA) thermosets coated with Chitlac-nAg, a nanocomposite system with antimicrobial properties. To avoid bacterial adhesion to dental devices and to reduce cytotoxicity against eukaryotic cells, we coated BisGMA/TEGDMA methacrylic thermosets with a new material, Chitlac-nAg, formed by stabilizing silver nanoparticles, which have well-known antimicrobial properties, with a polyelectrolyte solution containing Chitlac. Cytotoxicity, cell morphology, cell migration and inflammatory interleukine-6 (IL-6) and prostaglandin E2 (PGE2) secretion were evaluated. Our results showed that the cytotoxicity exerted on HGFs by our nanocomposite material was absent in our co-culture model, where fibroblasts are able to adhere and migrate. After 24 h thermosets coated with Chitlac as well as those coated with Chitlac-nAg exerted a minimal cytotoxic effect on HGFs, while after 48 h LDH release rises up 20%. Moreover the presence of S. mitis reduced this release in a greater amount with Chitlac-nAg coated thermosets. The secretion of IL-6 was significant in both Chitlac and Chitlac-nAg coated thermosets, but PGE2 production was minimal, suggesting that the IL-6 production was not related to an inflammatory response. Co-culture and the addiction of saliva did not influence IL-6 and PGE2 secretion. Data obtained in the present work suggest that Chitlac n-Ag coated thermosets could significantly improve the success rates of restorative dentistry, since they limit bacterial adhesion and are not toxic to HGFs.