RESUMEN
OBJECTIVE: To investigate the effect of low concentration of Wenyang Tonglin Decoction (WTD) on the binding conditions of R45 plasmid conjugative transfer under liquid phase conjugation and its mechanism. METHODS: Escherichia coli CP9 (R45) and Staphylococcus aureus RN450RF were cultured in medium containing WTD, and their minimum inhibitory concentration (MIC) values were obtained. Using promoter fusion technology, E. coli CP9 (R45) containing a promoter fusion was obtained. ß-Galactosidase activity of TrfAp and TrbBp was tested, and the mRNA expression of regulatory factors (TrbA, KorA, and KorB) was detected by real-time fluorescent quantitative polymerase chain reaction. RESULTS: The MIC of E. coli CP9 (R45) was 400 g/L and that of S. aureus RN450RF was 200 g/L. When the drug concentration in the culture medium was 200 g/L, the highest number of conjugants was (3.47 ±0.20) × 107 CFU/mL At 90 h of conjugation, the maximum number of conjugants was (1.15 ±0.06) × 108 CFU/mL When the initial bacterial concentration was 108 CFU/mL, the maximum number of conjugants was (3.47 ± 0.20) × 107 CFU/mL. When the drug concentration was 200 g/L, the ß-galactosidase activity of TrfAp and TrbBp significantly increased; the relative quantification of TrbA, KorA and KorB were significantly inhibited. CONCLUSION: Low concentration of WTD promoted the development of bacterial resistance by affecting promoters and inhibiting the expression of regulatory factors.
Asunto(s)
Medicamentos Herbarios Chinos , Escherichia coli , Pruebas de Sensibilidad Microbiana , Plásmidos , Staphylococcus aureus , Medicamentos Herbarios Chinos/farmacología , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/genética , Plásmidos/genética , Escherichia coli/efectos de los fármacos , Escherichia coli/genética , Conjugación Genética , Regiones Promotoras Genéticas/genética , Farmacorresistencia Bacteriana/genética , Farmacorresistencia Bacteriana/efectos de los fármacos , beta-Galactosidasa/metabolismo , beta-Galactosidasa/genética , Relación Dosis-Respuesta a Droga , ARN Mensajero/genética , ARN Mensajero/metabolismo , Regulación Bacteriana de la Expresión Génica/efectos de los fármacosRESUMEN
OBJECTIVE: To observe the alteration of QT dispersion (QTd) and QTc dispersion (QTcd) in hemodialysis patients after oral administration of Zhigancao Decoction (, Roasted Licorice Decoction, RLD). METHODS: To investigate the alteration of QTd and QTcd in 68 routine hemodialysis patients before and after hemodialysis with 12-lead electrocardiogram (ECG) after orally administrated RLD for 4 weeks. Blood was also taken for measurement of plasma electrolytes, liver function, renal function, hemoglobin (Hgb) and hematocrit (HCT). RESULTS: After hemodialysis, QTd and QTcd were prolonged evidently; the difference was significant between before and after hemodialysis (P<0.05). After RLD orally administrated for 4 weeks, QTd and QTcd only slightly increased after dialysis compared with pre-dialysis (P>0.05). The QTd and QTcd of the post-therapy-post-dialysis decreased significantly compared with the pre-therapy-post-dialysis (P<0.05). There were no other significant changes in other variables (post-therapy-pre-dialysis vs. pre-therapy-pre-dialysis, or post-therapy-post-dialysis vs. pre-therapy-post-dialysis;P>0.05). After therapy, the number of patients with supraventricular arrhythmia, occasional ventricular premature beat and multiple ventricular premature beat were decreased from 15 to 4, 10 to 2 and 7 to 1, respectively. CONCLUSION: RLD therapy not only lowered the increased QTd and QTcd after hemodialysis, but also displayed a safety profile.
Asunto(s)
Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/uso terapéutico , Electrocardiografía , Diálisis Renal , Adulto , Anciano , Demografía , Femenino , Humanos , Fallo Renal Crónico/tratamiento farmacológico , Fallo Renal Crónico/fisiopatología , Masculino , Persona de Mediana EdadRESUMEN
OBJECTIVE: To study the preventive effect of herbal formulation on experimental murine urinary tract infection (UTI) induced by Dr Escherichia coli 11128. METHODS: E. coli 11128 carrying Dr fimbriae was isolated from patients with chronic pyelonephritis. The minimal inhibitory concentration (MIC) value of herbal solution for E. coli 11128 was determined for further studies. Forty C3H/HeJ mice were divided into the herb-treated group (n=20, given Chinese herbs by gavage at an average dose of 20 g/kg body weight daily 3 days before inoculation), and control group (n=20, given the same amount of distilled water by gavage). Three and 6 days after infection, bacteria were counted in the urine and the kidneys of the mice. Kidney histopathologic changes were evaluated. Neutrophils infiltration and accumulation were detected. RESULTS: The MIC value of herbal solution was 0.1 g/mL for the E. coli 11128. In herb-treated mice, there was a significant reduction in bacterial counts in urine and colonization densities of kidneys. Microscopic studies revealed signs of inflammation in kidneys. In herb-treated mice, herbal administration resulted in significantly reduced neutrophilic infiltrates (P<0.05). The semi-quantitative scores for renal lesions were significantly lower (P<0.05). CONCLUSION: Prophylactic administration of herbal formulation potentiated the effect in partially preventing experimental murine ascending UTI.
Asunto(s)
Medicamentos Herbarios Chinos/uso terapéutico , Fitoterapia , Infecciones Urinarias/tratamiento farmacológico , Infecciones Urinarias/prevención & control , Animales , Modelos Animales de Enfermedad , Medicamentos Herbarios Chinos/farmacología , Escherichia coli/efectos de los fármacos , Infecciones por Escherichia coli/tratamiento farmacológico , Infecciones por Escherichia coli/prevención & control , Femenino , Riñón/efectos de los fármacos , Riñón/patología , Ratones Endogámicos C3H , Infecciones Urinarias/microbiologíaRESUMEN
BACKGROUND: A major cause of mortality in hemodialysis patients is cardiac disease. Most complementary and alternative therapies, including Chinese herbal medicine, have been useful in the treatment of cardiac disorders. MATERIALS AND METHODS: A 46 year old Asian woman with chronic renal failure was admitted to the clinic for hemodialysis. In the course of the fifth session of standard dialysis, she developed shock followed by a ventricular tachycardia which rapidly degenerated into cardiac arrest, from which she was resuscitated through cardio-pulmonary resuscitation. The following therapeutic strategies were applied: low discharge oxygen inhalation; stricter water and salt restriction; dialysate temperature set at 36.0 °C; rhEPO 3000u, per week, low molecular weight iron dextran, 200mg/day, intravenously for five days; the patient received Chinese herbal concoction orally. RESULTS: The patient obtained efficient standard dialysis without any cardiac syndrome. CONCLUSION: Chinese herbs are useful in the management of cardiac disorders in hemodialysis. Chinese herbs may provide more benefits by adjusting dialysis strategies.
Asunto(s)
Medicamentos Herbarios Chinos/administración & dosificación , Fallo Renal Crónico/terapia , Fitoterapia/métodos , Diálisis Renal/efectos adversos , Taquicardia Ventricular/terapia , Terapia Combinada , Femenino , Humanos , Persona de Mediana Edad , Taquicardia Ventricular/etiología , Resultado del TratamientoRESUMEN
Objective. To investigate the effects of Chinese herbal therapy on chronic tension-type headache. Method. 132 patients with chronic tension-type headache were enrolled in the study. All patients filled in headache questionnaire at baseline phase and 4, 8, and 12 weeks after baseline. As an alternative therapeutic method, the patients were orally administrated Chinese herbal concoction for ten days. Therapeutic effects were evaluated during 12 weeks of followup. Result. In the primary outcome analysis, mean headache scores were significantly lower in the group. Scores fell by 25%-40% during 12 weeks of followup. Patients fared significantly well for most secondary outcome measures. From baseline to 4-12 weeks of followup, the number of days with headache decreased by 6.8-9.5 days. Duration of each attack also significantly (P < 0.05) shortened from 5.3 hours at 4 weeks to 4.9 hours after 8 weeks of followup. Days with medication per four weeks at followup were lower than those at the baseline. The differences were significant (P < 0.05, 0.01) for all end points. Days with medication fell by 56.6% at 12 weeks. Conclusion. The study has provided evidence that Chinese herbal therapy can be clinically useful for the treatment of chronic tension-type headache.
RESUMEN
BACKGROUND: Clinical isolates of herb-resistant uropathogenic E. coli were isolated. It was possible that the virulence genotypes and phylogenetic background of E. coli differed between Chinese herb-resistant E. coli and -susceptible isolates. For this purpose, the prevalence of virulence factors (VFs) and phylogenetic background, with regard to Chinese herb resistance, among E. coli strains causing acute pyelonephritis from China were investigated. MATERIALS AND METHODS: E. coli isolates from patients with acute pyelonephritis were used in this study. Standard disc diffusion methodology was used to test the susceptibility of Chinese herbal concoction against E. coli strains. Multiplex PCR amplifications employed three markers (chuA, yjaA, and TSPE4.C2) to classify E. coli isolates into one of four phylogenetic groups (group A, B1, B2, or D). The isolates were also tested for 14 virulence-associated traits (VFs) of uropathogenic E. coli. RESULTS: A total of 115 E. coli strains were isolated. 79 (68.7%) were susceptible and 36 (31.3%) were resistant to the herbal concoction. 20.9% of the isolates encoded three or more of VFs for which they were screened, with 13.9% in susceptible isolates and 36.1% in resistant isolates. The key VFs (fyuA and/or iutA siderophores) present in >80% of isolates. The papA and papC adhesins were detected in the majority of resistant isolates (72.2% and 63.9% respectively). 78.5% of susceptible isolates belong to phylogenetic groups A, while 83.3% of resistant isolates belong to group B2. CONCLUSION: PapA and papC are significant VFs with an essential role in contributing to Chinese herb-resistance. Chinese herb-resistance is associated with a shift towards more virulent strains and B2 phylogenetic group.
Asunto(s)
Antibacterianos/farmacología , Medicamentos Herbarios Chinos/farmacología , Infecciones por Escherichia coli/microbiología , Pielonefritis/microbiología , Escherichia coli Uropatógena/efectos de los fármacos , Escherichia coli Uropatógena/genética , China , Proteínas de Escherichia coli/genética , Genotipo , Humanos , Pruebas de Sensibilidad Microbiana , Datos de Secuencia Molecular , Filogenia , Escherichia coli Uropatógena/clasificación , Escherichia coli Uropatógena/aislamiento & purificación , Virulencia , Factores de Virulencia/genéticaRESUMEN
BACKGROUND: In order to define the virulence factors between Chinese herb-resistant uropathogenic E. coli and susceptible strains, the UPEC isolates were classified into two groups according to its resistance to Chinese herbs. MATERIALS AND METHODS: The susceptibility profile of strains was determined by disk diffusion method. PCR systems were used to detect genes encoding papC, Aer, hly and cnf1. Isolated human urothelial cells were incubated in vitro and investigated with light microscope immunohistochemistry. Adhesion of E. coli to urothelial cells was studied in vitro. RESULTS: The results showed that, among the 105 UPEC isolates, 18 were resistant to the herbal concoction. Cnf1 and papC occurred in ≥66.7%, of herb-resistant isolates, while, hly and Aer occurred in 22.2% and 27.8% of strains respectively. Only one gene (Cnf1) occurred in >40%, of Herb-susceptible isolates. Other genes were also found in susceptible isolates: papC (20.7%), hly (11.5%), and Aer (6.9%). Light microscopy and immunochemical investigations demonstrated the normal pelvic transitional epithelial cells cultured. The adherence of strains in both groups increased in 30 min., and reached its peak at 60, (Susceptible E. coli) or 120 min., (Resistant E. coli). The adhesion of the susceptible bacteria to human uroepithelial cells was significantly lower compared with that of the resistant E. coli (p<0.05). CONCLUSION: These findings revealed that, Chinese herb-resistant uropathogenic E. coli isolates that are hemolytic, and have Aer, papC, hly, Cnf1 genes are more able to be uropathogenic and adherent.
Asunto(s)
Resistencia a Medicamentos , Medicamentos Herbarios Chinos , Infecciones por Escherichia coli/microbiología , Genes Bacterianos , Escherichia coli Uropatógena/patogenicidad , Urotelio/microbiología , Adhesión Celular , Medicamentos Herbarios Chinos/farmacología , Humanos , Escherichia coli Uropatógena/efectos de los fármacos , Escherichia coli Uropatógena/genética , Virulencia/genéticaRESUMEN
Multidrug-resistant Pseudomonas aeruginosa, which was only susceptible to colistin, was isolated from the urine of a patient with pyelonephritis. The isolates were confirmed as class A Extended-spectrum-ß-lactamase-producing isolates. A blaBEL allele was detected by multiplex polymerase chain reaction. The organisms were identified as ST235, serotype O11. Chinese herbal decoction was orally administered. The patient returned 8 days later with relief of symptoms. No P. aeruginosa was isolated from two urine samples taken after 1 week and 2 weeks from the end of herbal therapy. The case supports the notion that these Chinese herbs are useful in treating pyelonephritis caused by multidrug-resistant P. aeruginosa. Chinese herbal medicine has allowed another choice of treatment.
Asunto(s)
Extractos Vegetales/uso terapéutico , Infecciones por Pseudomonas/microbiología , Pseudomonas aeruginosa/efectos de los fármacos , Pielonefritis/microbiología , Anciano , Antibacterianos/uso terapéutico , Farmacorresistencia Bacteriana Múltiple , Femenino , Humanos , Infecciones por Pseudomonas/tratamiento farmacológico , Pseudomonas aeruginosa/aislamiento & purificación , Pielonefritis/tratamiento farmacológicoRESUMEN
A 48-year-old asian man was admitted to hospital for the consideration of hemodialysis (HD). An adequate site was decided on for the AVF after Doppler ultrasonography examination of radial artery and cephalic vein. The AVF was formed under local anaesthetic at his right wrist. When seen at the clinic appointment one month later, the AVF was not working. The subsequent Doppler ultrasonography examination revealed two straight shunts at draining vein. The failure of AVF is due to impaired outflow following reduction of the resistance between draining vein and peripheral venous system.
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Fístula Arteriovenosa , Diálisis Renal/métodos , Humanos , Masculino , Persona de Mediana Edad , Arteria Radial , Venas , Muñeca/irrigación sanguíneaRESUMEN
We report a case of continuous ambulatory peritoneal dialysis (CAPD)-related peritonitis. Burkholderia gladioli, as a plant pathogen, were cultured from peritoneal effluent. Peritonitis healed after intraperitoneal cefazolin and gentamicin. This case indicated that CAPD effluent may be contaminated by B. gladioli, causing CAPD-related peritonitis. On the other hand, it can be successfully treated with intraperitoneal antimicrobials without removal of the Tenckhoff catheter.
Asunto(s)
Infecciones por Burkholderia/patología , Burkholderia gladioli/aislamiento & purificación , Peritonitis/microbiología , Anciano , Humanos , Masculino , Diálisis Peritoneal Ambulatoria Continua/efectos adversosRESUMEN
BACKGROUND: Many Chinese herbal formulations are used in the treatment of urinary tract infection. Clinical isolates of Escherichia coli (E. coli) resistant to Chinese herbs have been identified rarely. Report here is a case in which Chinese herbs-resistant E. coli isolate was identified. CASE: The strain of E. coli C16 was isolated from urine of a 72-year-old woman with a diagnosis of pyelonephritis. Standard disc diffusion methodology was used to test the Chinese herbal decoction against E. coli C16. The minimum inhibitory concentration value was 0.1 g/mL. The minimum bactericidal concentration value was 0.2 g/mL. The decoction was orally administered for 4 weeks. RESULTS: The therapeutic regimen was well tolerated initially. At the end of 4 weeks, the disease relapsed. E. coli C16 was isolated again, which was resistant to herbal solution. CONCLUSIONS: This case illustrates that excessive exposure to Chinese herbs used in unchanging standard formulations can also lead to bacterial resistance. Appropriate clinical use of Chinese herbs is imperative.
Asunto(s)
Resistencia a Medicamentos , Tolerancia a Medicamentos , Medicamentos Herbarios Chinos , Infecciones por Escherichia coli/microbiología , Escherichia coli/efectos de los fármacos , Pielonefritis/microbiología , Infecciones Urinarias/microbiología , Administración Oral , Anciano , Antibacterianos/uso terapéutico , Medicamentos Herbarios Chinos/uso terapéutico , Escherichia coli/aislamiento & purificación , Infecciones por Escherichia coli/tratamiento farmacológico , Femenino , Humanos , Pruebas de Sensibilidad Microbiana , Fitoterapia , Pielonefritis/tratamiento farmacológico , Recurrencia , Infecciones Urinarias/tratamiento farmacológico , Orina/microbiologíaRESUMEN
OBJECTIVE: This study aimed at assessing the adequacy of blood pressure (BP) control with the Chinese herbal fomentation at Yongquan point (K1). METHOD: A total of 51 participants as a group were enrolled. In 10 participants as the subgroup, BP data were compared with those simultaneously recorded from the radial artery of the same side. After the baseline examination, each enrolled patient participated in one session lasting 24 hours, during which Yongquan point (K1) of both sides were fomented with a mixture of Chinese herbs. The technique of 24-hour Ambulatory BP monitoring (ABPM) was repeated at baseline and on the day after the therapy. RESULTS: The systolic and diastolic BP measured intra-arterially during the test were, on average, similar to those measured noninvasively. Patients obtained a 6.0mmHg reduction for systolic BP and a 4.8mmHg reduction for diastolic BP during daytime. The rate of adverse events was 2.0% in the group. CONCLUSION: Our data demonstrated that BP recording by the ABPM method in our study provided a relatively accurate estimate of the average radial BP of the population. So the conclusion, "Chinese herbal fomentation at Yongquan point (K1) of both sides has some hypotensive potentials" was more reliable.
Asunto(s)
Puntos de Acupuntura , Antihipertensivos/uso terapéutico , Presión Sanguínea/efectos de los fármacos , Medicamentos Herbarios Chinos/uso terapéutico , Hipertensión/tratamiento farmacológico , Fitoterapia , Administración Tópica , Adulto , Antihipertensivos/farmacología , Monitoreo Ambulatorio de la Presión Arterial , Medicamentos Herbarios Chinos/farmacología , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
OBJECTIVE: The goal of this research was to assess the adequacy of blood pressure (BP) control by applying Chinese herbal fomentation (a type of thermotherapy) at the Yongquan point (K1). METHODS: A total of 102 patients with hypertension, ages 39-55, were included in this study. After a baseline examination, each enrolled patient participated in one session per day lasting 24 hours for 3 continuous days, during which the Yongquan point (K1) on both sides of the body were fomented (given thermotherapy) with a mixture of Chinese herbs. In addition, 24-hour ambulatory BP (ABP) monitoring (ABPM) was repeated at baseline and on the day after the therapy. RESULTS: Patients had significantly lower daytime systolic and diastolic BP post-treatment. There were no significant changes in 24-hour, or night-time systolic BP (SBP), diastolic BP (DBP), or mean arterial BP, and in pulse pressure after therapy. The rate of adverse events was 0.98% in these patients. CONCLUSIONS: The regimen sustained SBP and DBP control during the day for the majority of patients. This study suggested that a Chinese herbal fomentated at the Yongquan point (K1) of both sides of the body has some hypotensive potential.
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Puntos de Acupuntura , Antihipertensivos/uso terapéutico , Presión Sanguínea/efectos de los fármacos , Medicamentos Herbarios Chinos/uso terapéutico , Hipertensión/tratamiento farmacológico , Fitoterapia , Terapia por Acupuntura/métodos , Administración Tópica , Adulto , Medicamentos Herbarios Chinos/administración & dosificación , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
We have used reduced doses of Chinese herbs for estimation of urinary tract infections (UTIs) patients with stable impairment of renal function. A total of 33 adult female patients with moderately impaired renal function and symptomatic UTIs were included in this study. Urine cultures were carried out. Patients were monitored clinically and with various laboratory tests. Chinese herbal concoction divided by milligrams of creatinine per 100 ml were orally administrated for ten days. Three patients were excluded from final analysis. Most of the patients responded symptomatically to treatment. Chinese herbs eradicated the primary pathogen in 68.7% of the patients at the day 10 of treatment. Two patients relapsed (one had abbreviated courses of therapy) 6 to 8 days posttreatment. Organisms which recurred included Escherichia coli and Pseudomonas aeruginosa. Bacterial reinfections occurred 5 to 8 days posttreatment in four patients. Adverse reactions observed among the 30 patients were rare. Nausea (6.67%) and mild elevation of hepatic enzymes (3.33%) were probably drug related. Nausea disappeared when the therapy ended. Elevated hepatic enzymes resumed at the 2-week follow-up. Two patients demonstrated slight increases in serum creatinine on day 10 of treatment. One patient had a 12.5% elevation over baseline and the other had a 13.0% elevation. Serum creatinine values had improved in these two patients at 4-week follow-up. Chinese herbal medicine was effective and safe in the treatment of UTIs with renal insufficiency.
Asunto(s)
Medicamentos Herbarios Chinos/uso terapéutico , Magnoliopsida , Fitoterapia , Insuficiencia Renal/complicaciones , Infecciones Urinarias/tratamiento farmacológico , Adulto , Anciano , Creatinina/sangre , Medicamentos Herbarios Chinos/efectos adversos , Medicamentos Herbarios Chinos/farmacología , Escherichia coli , Femenino , Humanos , Hígado/efectos de los fármacos , Hígado/enzimología , Persona de Mediana Edad , Náusea/etiología , Pseudomonas aeruginosa , Recurrencia , Insuficiencia Renal/sangre , Infecciones Urinarias/complicaciones , Infecciones Urinarias/microbiologíaRESUMEN
OBJECTIVES: The study objectives were to examine the effects of Chinese herbal fomentation on radiocephalic arteriovenous fistula (AVF) maturation. METHODS: All patients' preoperative evaluation, operative procedure, and follow-up information records were reviewed to identify patients undergoing radiocephalic AVF procedures for maintenance hemodialysis. All patients underwent preoperative vessel ultrasound. The ultrasound examinations were repeated every 2 months after the operative procedure. From the day after the operation, the forearm of the patient is fomented with a mixture of Dan Shen and Hong Hua in dry powdered form dissolved in fresh warm water. The fomentation lasted for 60 minutes, once a day for 28 consecutive days. RESULTS: Sixty-four (64) specific access site operations were yielded in 62 patients for analysis. Forty-nine (49; 76.6%) of the 64 fistulas were considered sonographically mature and 15 (23.4%) were deemed sonographically immature. The sonographically mature fistulas (n=46) that were suitable for puncture had blood flow of 655±132 mL/minute, and venous diameter of 4.5±0.3 mm. The most frequent complication was thrombosis before first use, which accounted for 6 AVFs failures to mature (0.16 per patient-year). There were two hospitalizations for fistula-related bacteremia in the group (0.05 per patient-year). CONCLUSIONS: The Chinese herbal fomentation reduced the frequency of early thrombosis of new AVFs and was accompanied by an increase in the proportion of AVFs that were suitable for dialysis.
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Derivación Arteriovenosa Quirúrgica , Medicamentos Herbarios Chinos/farmacología , Arteria Radial/efectos de los fármacos , Diálisis Renal/métodos , Trombosis/prevención & control , Grado de Desobstrucción Vascular , Administración Tópica , Adulto , Bacteriemia , Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/uso terapéutico , Femenino , Hospitalización , Humanos , Masculino , Medicina Tradicional China , Persona de Mediana Edad , Fitoterapia , Arteria Radial/diagnóstico por imagen , Arteria Radial/cirugía , Estudios Retrospectivos , Salvia miltiorrhiza , Trombosis/etiología , Ultrasonografía , Venas/diagnóstico por imagen , Venas/efectos de los fármacos , Venas/cirugía , Cicatrización de HeridasRESUMEN
A 56-year-old Asian woman was admitted to hospital for the consideration of hemodialysis (HD). A right femoral dialysis catheter was inserted for HD. Three months after removal of catheter, she was admitted because of right inguinal swelling. A thrill and bruit were felt and heard at the inguinal area. Color Doppler detected a fistula between right superficial femoral artery and right common femoral vein and subsequently confirmed by contrast enhanced computed tomography scan and 3-dimensional reconstruction with computed tomography. At surgery, a 4-mm-diameter fistula was found between the right superficial femoral artery and right common femoral vein. A primary closure of both defects in the artery and vein was then carried out. A follow-up digital vascular study 3 months after surgical repair was normal. In conclusion, nephrologist should have a heightened awareness to the potential of this complication and should at least document a normal exam following the removal of femoral catheters.
Asunto(s)
Fístula Arteriovenosa/cirugía , Cateterismo/efectos adversos , Fallo Renal Crónico/complicaciones , Diálisis Renal/métodos , Femenino , Vena Femoral/cirugía , Humanos , Persona de Mediana EdadRESUMEN
We assessed the effects of Chinese herbs on the uncomplicated urinary tract infections (UTIs) in women caused by fluoroquinolone-resistant strains. A total of 56 pre-menopausal women with uncomplicated UTIs caused by fluoroquinolone-resistant strains were included. Urine cultures were carried out. All organisms were proved to be fluoroquinolone-resistant at baseline. The patients were orally administrated Chinese herbal concoction for ten days. Chinese herbal concoction eradicated the primary pathogen in 71.4% of the patients at the 1-week follow-up. Among the 20 patients who had bacteriologic failures in the Day 5 of treatment, 2 developed superinfection. Of the failures in the group, Proteus mirabilis, Staphylococcus epidermidis and Providencia rettgeri were implicated in 50.0%, 50.0% and 100.0% of the failures, respectively. The clinical outcomes were also good, with cure or improvement for more than 80% of all subjects. About 14% of the study subjects reported at least one potential adverse event. The adverse events most frequently reported were nausea and diarrhea. All patients tolerated the symptoms. The adverse reactions did not prevail after discontinuation of the medication. Chinese herbal therapy may be an acceptable alternative for the treatment of uncomplicated UTIs caused by fluoroquinolone-resistant uropathogens.
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Antiinfecciosos Urinarios/uso terapéutico , Infecciones Bacterianas/tratamiento farmacológico , Farmacorresistencia Bacteriana , Medicamentos Herbarios Chinos/uso terapéutico , Fluoroquinolonas/uso terapéutico , Infecciones Urinarias/tratamiento farmacológico , Adulto , Infecciones Bacterianas/diagnóstico , Infecciones Bacterianas/microbiología , Femenino , Estudios de Seguimiento , Humanos , Pruebas de Sensibilidad Microbiana , Persona de Mediana Edad , Fitoterapia , Premenopausia , Resultado del Tratamiento , Infecciones Urinarias/diagnóstico , Infecciones Urinarias/microbiología , Adulto JovenRESUMEN
The aim of this study is to evaluate Chinese herbs' efficacy on adhesive properties of Escherichia coli (E. coli). The effects of Chinese herbal solution on the hemagglutination and adhesion by E. coli strain were studied. E. coli C16 was isolated from a patient with urinary tract infection. The MIC value of herbal solution for the E. coli C16 was 0.1g/ml. The MBC value was 0.2g/ml. The effects of herbal solution on the hemagglutination abilities of E. coli C16 were dependent on the herbal solution used. The strain C16 lost half of its hemagglutination abilities when the herbal solution concentration was at MIC (0.05g/ml). Herbal solution decreased the adherence of strain C16 in a dose-dependent way. The numbers of adherent bacteria were reduced to 45% of the control values after growth with herbal solution at MIC. The results show that anti-adhesion is one mode of action for Chinese herbs used against pathogens.
Asunto(s)
Adhesión Bacteriana/efectos de los fármacos , Medicamentos Herbarios Chinos/farmacología , Infecciones por Escherichia coli/microbiología , Escherichia coli/efectos de los fármacos , Hemaglutinación/efectos de los fármacos , Infecciones Urinarias/microbiología , Animales , Relación Dosis-Respuesta a Droga , Escherichia coli/fisiología , Pruebas de Hemaglutinación , Humanos , Pruebas de Sensibilidad MicrobianaRESUMEN
Our study aimed to investigate the effects of acupuncture on diabetic peripheral neuropathy. We compared 42 cases treated with acupuncture with 21 cases exposed to sham acupuncture and observed the effects on nerve conduction velocity and a variety of subjective symptoms associated with diabetic peripheral neuropathy. Three of the six measures of motor nerves, and two measures of sensory function, demonstrated significant improvement (p < 0.05) over the 15-day treatment period in the acupuncture group, while no motor or sensory function significantly improved in the sham acupuncture group. There were also significant differences in vibration perception threshold between the groups (p < 0.05) and when compared to the baseline levels (p < 0.01) in the acupuncture group. Acupuncture was significantly more effective than sham for treatment of numbness of the lower extremities, spontaneous pain in the lower extremities, rigidity in the upper extremities and alterations in temperature perception in the lower extremities after therapy. Our pilot study has, therefore, provided evidence that acupuncture may be clinically useful for the radical treatment of diabetic peripheral neuropathy.