RESUMEN

We have cloned three cDNA isoforms of focal adhesion kinase (FAK) from the sea urchin, Lytechinus variegatus. The sea urchin FAK is more closely related to FAK from other deuterostomes than from invertebrate protostomes or to cell adhesion kinase beta (CAKbeta/Pyk2/FAK2). FAK is expressed in all cells of sea urchin embryos by the 120-cell stage and strongly in blastulae. Phospho-FAK concentrates on basal surfaces of epithelial cells in early blastulae and occurs in syncytial cables of primary mesenchyme cells (PMC). Inhibition of FAK by constructs of FAK-related non-kinase delays blastocoel expansion and early PMC ingression. These results suggest that FAK has roles in cell adhesion and in the shape and integrity of the epithelial cells in sea urchin embryos.

Asunto(s)

Embrión no Mamífero/enzimología , Proteínas Tirosina Quinasas/genética , Secuencia de Aminoácidos , Animales , Adhesión Celular/fisiología , Embrión no Mamífero/embriología , Embrión no Mamífero/metabolismo , Proteína-Tirosina Quinasas de Adhesión Focal , Expresión Génica/fisiología , Genes Reporteros , Inmunohistoquímica , Datos de Secuencia Molecular , Fosforilación , Filogenia , Proteínas Tirosina Quinasas/metabolismo , Erizos de Mar