RESUMEN
The complete genome sequence of Streptomyces fulvissimus (DSM 40593), consisting of a linear chromosome with a size of 7.9Mbp, is reported. Preliminary data indicates that the chromosome of S. fulvissimus contains 32 putative gene clusters involved in the biosynthesis of secondary metabolites, two of them showing very high similarity to the valinomycin and nonactin biosynthetic clusters. The availability of genome sequence of S. fulvissimus will contribute to the evaluation of the full biosynthetical potential of streptomycetes.
Asunto(s)
Genoma Bacteriano/genética , Streptomyces/genética , Datos de Secuencia MolecularRESUMEN
The paper is focused on the primary analysis of the potential genes of primary response to IFNalpha, predicted by the program COTRASIF elaborated in our laboratory. Two web-instruments FatiGO and GOTM were applied for this purpose; the literature search was carried out using IHOP; genes were classified according to the conceptual diagram "Gene Ontology" (GO) and were ranked according to their first priority for the experimental validation. On the basis of conducted analysis 162 genes of potential primary response to IFNalpha were subdivided into two groups--61 genes, for which the experimental data of their responsibility to IFNalpha were found and 101 genes for which such data are absent. We have performed the functional analysis of the "unknown" genes according to GO. The enriched category "Synapse part" encountering three genes of Rattus norvegicus: PRKCA-binding of protein, NMDAR-L and GABAA--receptor subunit alpha-2 were revealed. Among 162 genes the enriched category "Immune response" was detected with Mbl1 gene, which is reckoned among "unknown" genes. The four designated genes are chosen as the candidates for the prior validation.