RESUMEN
The case of a giant-cell epulis as an initial feature of primary hyperparathyroidism is presented. A 58-year-old woman appeared for dental treatment with a buccal alveolar swelling that was confirmed by means of biopsies to be a multinucleated giant-cell lesion. High calcium levels warranted investigation for a parathyroid adenoma, which was located in an ectopic position and surgically removed. This case delineates the importance of referring patients with oral lesions of doubtful cause to specialists for further diagnostic workup.
Asunto(s)
Enfermedades de las Encías/etiología , Granuloma de Células Gigantes/etiología , Hiperparatiroidismo/complicaciones , Adenoma/complicaciones , Adenoma/diagnóstico , Biopsia , Calcio/sangre , Coristoma/diagnóstico , Femenino , Enfermedades de las Encías/patología , Granuloma de Células Gigantes/patología , Humanos , Hiperparatiroidismo/sangre , Maxilar , Enfermedades del Mediastino/diagnóstico , Persona de Mediana Edad , Glándulas Paratiroides , Neoplasias de las Paratiroides/complicaciones , Neoplasias de las Paratiroides/diagnósticoRESUMEN
A new differential medium, CHROMagar Candida, for the isolation of clinically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. The recovery of yeasts on the medium was not significantly different from the recovery on Sabouraud dextrose agar. The identities of 450 green colonies on CHROMagar Candida, presumptively identified as Candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-N-acetylgalactosaminidase. Candida tropicalis also formed distinctive colonies, and other yeasts including Candida (Torulopsis) glabrata, Candida Parapsilosis, Candida Magnoliae, Candida lusitaniae, Candida Famata, Candida kefir, and Saccharomyces cerevisiae were readily distinguished from C. albicans and C. tropicalis isolates. CHROMagar Candida is a very useful medium, and its use will facilitate the study of yeasts associated with dental diseases.
Asunto(s)
Candida/aislamiento & purificación , Medios de Cultivo , Enfermedades de la Boca/microbiología , Saccharomyces cerevisiae/aislamiento & purificación , Candidiasis Bucal/microbiología , Caries Dental/microbiología , Dentadura Completa , Humanos , Boca Edéntula/microbiologíaRESUMEN
The antibacterial activity of acacia gum was assessed using fresh isolates and reference strains of Actinobacillus actinomycetemcomitans, Capnocytophaga spp., Porphyromonas gingivalis, Prevotella intermedia and Treponema denticola. A fine aqueous suspension of gum was produced by sonication and then a soluble fraction isolated by centrifugation and membrane filtration. These preparations were incorporated into columbia agar at doubling concentrations. Growth of P. gingivalis and P. intermedia cultures on the agar was inhibited by whole gum sonicate at concentrations of 0.5-1.0% w/v. Both species showed reduced susceptibility when horse blood was present in the agar. The gum soluble fraction did not inhibit growth of any bacterial culture. The effect of acacia on bacterial proteases was examined with cell sonicates from log phase broth cultures. Enzyme activities were determined by fluorimetric assay with various synthetic peptide substrates. Most protease activities reduced in the presence of 0.5% w/v gum sonicate, with the trypsin-like activities of P. gingivalis and P. intermedia proving most sensitive. The gum soluble fraction was nearly always less inhibitory than the sonicate. The action of acacia gum against suspected periodontal pathogens and their enzymes suggests that it may be of clinical value.