RESUMEN

The complete mitochondrial genome sequence of Great tit Parus major was sequenced used polymerase chain reaction (PCR), long-and-accurate PCR and directly sequencing by primer walking. The Genbank accession was KP137624. The entire mitochondrial genome of P. major is a circular molecule of 16,776 bp in length and the content of A, T, C and G were 29.68%, 22.63%, 33.56% and 14.13%, respectively. The complete mitochondrial genome of P. major contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, plus 1 control regions and was similar to most of the other Aves birds in gene arrangement and composition. The complete mitochondrial genome of P. major could provide a useful data for resolving phylogenetic relationship problems related to Parus and P. major subspecies complex.

Asunto(s)

Aves/genética , Genoma Mitocondrial , Animales , Composición de Base , ADN/química , ADN/aislamiento & purificación , ADN/metabolismo , ADN Mitocondrial/química , ADN Mitocondrial/aislamiento & purificación , ADN Mitocondrial/metabolismo , Sistemas de Lectura Abierta/genética , ARN Ribosómico/química , ARN Ribosómico/aislamiento & purificación , ARN Ribosómico/metabolismo , ARN de Transferencia/química , ARN de Transferencia/aislamiento & purificación , ARN de Transferencia/metabolismo , Análisis de Secuencia de ADN