RESUMEN

A sensitive method for determining exposure to the chemical warfare agent VX is described in which the biomarker ethyl methylphosphonofluoridate (VX-G) is measured in red blood cells (RBCs) following treatment with fluoride ion using isotope-dilution gas chromatography-tandem mass spectrometry. The analyte was isolated via solid-phase extraction and detected using ammonia chemical ionization in the multiple reaction monitoring mode. A good linear relationship was obtained in the quantitative concentration range of 4 ng/mL to 1000 ng/mL with an absolute detection limit of < 1 pg on column. The method has been applied to the analysis of RBCs from a laboratory worker accidentally exposed to VX vapor. Detection and quantitation of VX-G were possible in samples taken as late as 27 days following exposure.

Asunto(s)

Monitoreo del Ambiente/métodos , Eritrocitos/química , Cromatografía de Gases y Espectrometría de Masas/métodos , Compuestos Organotiofosforados/sangre , Espectrometría de Masas en Tándem/métodos , Acetilcolinesterasa/química , Acetilcolinesterasa/metabolismo , Biomarcadores/análisis , Biomarcadores/sangre , Sustancias para la Guerra Química/análisis , Sustancias para la Guerra Química/metabolismo , Inhibidores de la Colinesterasa/análisis , Inhibidores de la Colinesterasa/sangre , Inhibidores de la Colinesterasa/metabolismo , Exposición a Riesgos Ambientales/análisis , Eritrocitos/enzimología , Fluoruros/química , Humanos , Compuestos Organotiofosforados/análisis , Compuestos Organotiofosforados/metabolismo , Compuestos de Potasio/química , Extracción en Fase Sólida/métodos