RESUMEN
A spectroscopic device for monitoring the temperature of aqueous solutions is presented. It uses a 950 nm light emission diode as light source and two photodiodes as detectors. Temperature is monitored following the thermally induced absorbance changes of the water-OH second overtone (approximately 960 nm). A linear response between the light absorbed by an aqueous solution and its temperature is found in the range from 15 to 95 degrees C. A prediction error of 0.1 degrees C and a precision of 0.07 degrees C in temperature measurement can be achieved. Up to 0.1 M of electrolyte concentration can be present in the solution without significantly affecting the temperature measurement. Different strategies, such as remote (noninvasive) or in situ (using a fiber-optic probe) temperature measurement, are shown, and their relative advantages are discussed.
RESUMEN
BACKGROUND: Cathepsin B (CB) is a lysosomal cysteine proteinase synthesized as a zymogen of 39-47 kilodaltons (kD), which is subsequently converted into an active single- chain form of 33 kD (CB33) and, by additional processing, into the active 2-chain form containing a heavy chain of 27-29 kD (CB(27-29)) and a light chain of 4-6 kD. Increased or altered CB expression has been documented in a variety of tumor cells, but to the authors' knowledge only one study published to date has reported clinicopathologic significance for CB in transitional cell carcinoma (TCC) of the bladder. METHODS: In this work, CB expression was determined by Western blot analysis in TCC bladder tissue from 30 patients. Nontumor bladder tissue was also analyzed for CB expression. RESULTS: The study results demonstrate higher expression of CB in TCC invasive tumors than in superficial bladder carcinoma. Furthermore, whereas normal bladder only expressed the 29-kD CB protein, tumor and peritumoral tissue demonstrated the 27- to 29-kD CB form. Immunohistochemical staining did not evidence changes in CB localization between tumor and nontumor tissue. CONCLUSIONS: According to the results of the current study, bladder tumor progression appears to be associated with quantitative changes in CB protein expression, as well as with qualitative changes related to the type of CB expressed.