RESUMEN
Children whose parents are diagnosed with alcohol use problems are exposed to genetic and environmental risk factors and face a greater risk of developing mental health and behavioral problems and a higher risk of alcohol use. In this study, we present the case of a father and his son, both diagnosed with alcohol use disorder, who both attended, 12 years apart, the Inpatient Alcohol Treatment Program of the Alcohol Treatment Unit, in the Psychiatric Hospital of Attica, in Athens. The Alcohol Treatment Unit offers two inpatient treatment programs that have been operating since 1996 and are based on the principles of the Therapeutic Community. It was the first time that both a father and son coming from the same family attended one of these programs. The aim of this study is to extract useful information regarding the dynamics of a family in which alcohol dependence is transferred from generation to generation. Therapists try to decode this transference and interpret attitudes and behaviors under these circumstances.
Asunto(s)
Alcoholismo , Humanos , Masculino , Consumo de Bebidas Alcohólicas/efectos adversos , Padre/psicología , Alcoholismo/terapia , Planificación en SaludRESUMEN
The Mediterranean fruit fly Ceratitis capitata is an insect capable of wreaking extensive damage to a wide range of fruit crops. Protein kinase CK2 is a ubiquitous Ser/Thr kinase that is highly conserved among eukaryotes; it is a heterotetramer composed of two catalytic (α) and a dimer of regulatory (ß) subunits. We present here the construction of the cDNA molecules of the CK2α and CK2ß subunits from the medfly C. capitata by the 5'/3' RACE and RT-PCR methods, respectively. CcCK2α catalytic subunit presents the characteristic and conserved features of a typical protein kinase, similar to the regulatory CcCK2ß subunit, that also possess the conserved features of regulatory CK2ß subunits, as revealed by comparison of their predicted amino acid sequences with other eukaryotic species. The recombinant CcCK2α and CcCK2ß proteins were purified by affinity chromatography to homogeneity, after overexpression in Escherichia coli. CcCK2α is capable to utilize GTP and its activity and is inhibited by polyanions and stimulated by polycations in phosphorylation assays, using purified acidic ribosomal protein P1 as a substrate.