RESUMEN
The present study was undertaken to investigate the ability of a drug curcumin-loaded polymer to inhibit the growth of cervical cancer cells by enhancing the anti-cancer efficiency of curcumin. We synthesized poly(methacryloyl beta-alanine) (PMBA) as a nanocarrier by radical polymerization in supercritical CO2. The results showed that the curcumin encapsulated and folic acid (FA)-treated PMBA (Poly@Cur-FA) for 24 h activated the reactive oxygen species-mediated programmed cell death machinery in HeLa cells. This remarkable effect of Poly@Cur-FA treatment was visualized using different fluorescent probes, which demonstrated that the Poly@Cur-FA treatment disrupted the cell membrane, as also supported by scanning electron microscopy observations. The effect of Poly@Cur-FA dispersion on the cells was observed under a transmission electron microscope. Further, the HeLa cells were treated with the polymer encapsulated curcumin and Bcl2 siRNA (Pol-Cur-siRNA) for 24 h, which effectively suppressed the Bcl2 and simulated the autophagic pathway. This co-delivery system was designed to inhibit curcumin efflux and can enhance the treatment efficacy by targeting multiple signaling pathways, including cell cycle, apoptotic, and autophagic pathways. Collectively, the Pol-Cur-siRNA system appears to offer an efficient combinational therapeutic strategy that might overcome the problems associated with the chemosensitivity against the standard synthetic anti-cancer drugs. To support the experimental data, an artificial neural network model was developed to foresee the drug and gene release behaviors.
Asunto(s)
Antineoplásicos , Curcumina , Nanopartículas , Neoplasias del Cuello Uterino , Aminoácidos , Antineoplásicos/química , Dióxido de Carbono , Curcumina/química , Portadores de Fármacos/química , Femenino , Ácido Fólico/química , Células HeLa , Humanos , Nanopartículas/química , Polímeros/uso terapéutico , Proteínas Proto-Oncogénicas c-bcl-2/genética , ARN Interferente Pequeño , Neoplasias del Cuello Uterino/tratamiento farmacológico , Neoplasias del Cuello Uterino/genéticaRESUMEN
Highly effective antimicrobial agents are needed to control the emergence of new bacterial strains, their increased proliferation capability, and antibacterial resistance that severely impact public health, and several industries including water, food, textiles, and oil and gas. Recently, bimetallic nanoparticles, formed via integration of two different metals, have appeared particularly promising with antibacterial efficiencies surpassing that of monometallic counterparts due to synergistic effects, broad range of physiochemical properties, and diverse mechanisms of action. This work aims to provide a review on developed bimetallic and supported bimetallic systems emphasizing in particular on the relation between synthesis routes, properties, and resulting efficiency. Bimetallic nanostructures on graphene, zeolites, clays, fibers, polymers, as well as non-supported bimetallic nanoparticles are reviewed, their synthesis methods and resulting properties are illustrated, along with their antimicrobial activity and potential against different strains of microbes.
RESUMEN
We report a case of vesicocervical fistula following intrauterine device (Lippes loop) erosion following insertion 45 years ago. Vesicocervical fistula was suspected clinically. Ultrasound and magnetic resonance imaging confirmed the presence of a foreign body. Biopsy and magnetic resonance imaging were performed to rule out malignancies of the urogenital tract. The fistula was managed by laparotomy, hysterectomy, and bladder flap closure. We report this case because of its rarity.