RESUMEN
A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based ELISA could be used as an alternative to MAT for the diagnosis of bovine leptospirosis.
Asunto(s)
Antígenos Bacterianos/química , Proteínas de la Membrana Bacteriana Externa/química , Enfermedades de los Bovinos/microbiología , Leptospira interrogans/aislamiento & purificación , Leptospirosis/veterinaria , Lipoproteínas/química , Animales , Anticuerpos Antibacterianos/sangre , Antígenos Bacterianos/biosíntesis , Antígenos Bacterianos/genética , Proteínas de la Membrana Bacteriana Externa/biosíntesis , Proteínas de la Membrana Bacteriana Externa/genética , Bovinos , Enfermedades de los Bovinos/sangre , Ensayo de Inmunoadsorción Enzimática/veterinaria , Leptospirosis/sangre , Leptospirosis/microbiología , Lipoproteínas/biosíntesis , Lipoproteínas/genética , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Sensibilidad y EspecificidadAsunto(s)
Proteínas de la Membrana Bacteriana Externa/genética , Leptospira interrogans serovar canicola/genética , Secuencia de Aminoácidos , Proteínas de la Membrana Bacteriana Externa/química , Secuencia de Bases , Clonación Molecular , Regulación Bacteriana de la Expresión Génica , Datos de Secuencia Molecular , FilogeniaAsunto(s)
Antígenos Bacterianos , Proteínas de la Membrana Bacteriana Externa , Enfermedades de los Bovinos/diagnóstico , Leptospira interrogans serovar canicola/inmunología , Leptospirosis/veterinaria , Proteínas Recombinantes , Animales , Antígenos Bacterianos/inmunología , Proteínas de la Membrana Bacteriana Externa/inmunología , Bovinos , Enfermedades de los Bovinos/microbiología , Leptospirosis/diagnóstico , Lipoproteínas/inmunología , Conejos , Sensibilidad y EspecificidadRESUMEN
The efficacy of a recombinant leptospiral lipoprotein LipL41 as an antigen for conducting enzyme-linked immunosorbent assay (ELISA) for diagnosis of bovine leptospirosis was evaluated. Using known positive and known negative cattle sera the recombinant antigen was found to be highly reactive in the concentration of 100 ng/well. Using a total of 321 field cattle sera the sensitivity of ELISA as compared to microscopic agglutination test (MAT) was calculated to be 100% whereas the specificity was 85.3%. The seropositivity of leptospirosis among bovine population was found to be 21.18% having the predominance of serovars Sejroe and Pomona. It was concluded that rLipL41 protein could be a putative diagnostic candidate for serodiagnosis of bovine leptospirosis.