RESUMEN
In this study, we report a new class of metallo-surfactant assisted silver nanoparticle produced by reduction process via AgNO3 solution and extract of Turnera Subulata (TS) in aqueous which act as reducing and metallo-surfactant [Co(ip)2(C12H25NH2)2](ClO4)3 (ip = imidazo[4,5-f][1,10]phenanthroline) act as stabilizing agent. In this study the silver nanoparticles produced using Turnera Subulata extract has showed yellowish brown color formation and an absorption peak at 421 nm signaling the biosynthesis of silver nanoparticles. The presence of functional groups in the plant extracts were identified by FTIR analysis. In addition, the effects of ratio, changing the concentration of the metallo surfactant, TS plant leave extract, metal precursors, and pH of the medium have been investigated on the scale of the Ag nanoparticles. Spherical shaped, crystalline in nature and â¼50 nm sized particles were recorded using TEM and DLS analysis. Furthermore, the mechanistic insights into cysteine and dopa detection by silver nanoparticles were investigated using HR-TEM analysis. This induces aggregation in stable silver nanoparticles owing to selective and strong interaction of -SH group of cysteine with silver nanoparticle surface. The biogenic Ag NPs are found to be highly sensitive to amino acids of dopa and cysteine with the diagnosis maximum for both amino acids as low as 0.9 µM (dopa) and 1 µM (cysteine) under optimized conditions.
Asunto(s)
Aminoácidos , Nanopartículas del Metal , Colorimetría , Nanopartículas del Metal/química , Cisteína/química , Plata/química , Tensoactivos , Extractos Vegetales/química , DihidroxifenilalaninaRESUMEN
The kinetics of outer sphere electron transfer reaction of surfactant cobalt(III) complex ions, cis-[Co(en)2(C12H25NH2)2](3+) (1), cis-[Co(dp)2(C12H25NH2)2](3+) (2), cis-[Co(trien)(C12H25NH2)2](3+) (3), cis-[Co(bpy)2(C12H25NH2)2](3+) (4) and cis-[Co(phen)2(C12H25NH2)2](3+) (5) (en: ethylenediamine, dp: diaminopropane, trien : triethylenetetramine, bpy: 2,2'-bipyridyl, phen: 1,10-phenanthroline and C12H25NH2 : dodecylamine) have been interrogated by Fe(2+) ion in ionic liquid (1-butyl-3-methylimidazoliumbromide) medium at different temperatures (298, 303, 308, 313, 318 and 323K) by the spectrophotometry method under pseudo first order conditions using an excess of the reductant. Experimentally the reactions were found to be of second order and the electron transfer as outer sphere. The second order rate constant for the electron transfer reaction in ionic liquids was found to increase with increase in the concentration of all these surfactant cobalt(III) complexes. Among these complexes (from en to phen ligand), complex containing the phenanthroline ligand rate is higher compared to other complexes. By assuming the outer sphere mechanism, the results have been explained based on the presence of aggregated structures containing cobalt(III) complexes at the surface of ionic liquids formed by the surfactant cobalt(III) complexes in the reaction medium. The activation parameters (enthalpy of activation ΔH() and entropy of activation ΔS()) of the reaction have been calculated which substantiate the kinetics of the reaction.
Asunto(s)
Cobalto/química , Complejos de Coordinación/química , Líquidos Iónicos/química , Tensoactivos/química , Quelantes/química , Transporte de Electrón , Interacciones Hidrofóbicas e Hidrofílicas , Iones , Cinética , Oxidación-ReducciónRESUMEN
A new surfactant cobalt(III) complex, cis-[Co(dpqc)2(DA)2](ClO4)3, where dpqc = dipyrido[3,2-a:2',4'-c](6,7,8,9-tetrahydro)phenazine and DA = dodecylamine, has been synthesized and characterized by elemental analysis, UV-Visible, IR and NMR spectra. The critical micelle concentration (CMC) value of this surfactant cobalt(III) complex in aqueous solution was obtained from conductance measurements. The conductivity data (at 303, 308, 313, 318 and 323 K) were used for the evaluation of the temperature-dependent CMC and the thermodynamics of micellization (ΔG(0) m, ΔH(0) m and ΔS(0) m). Absorption, fluorescence, cyclic voltammetry, circular dichroism and viscosity experiments have been carried out to study the interaction of the surfactant cobalt(III) complex with DNA and RNA. The results suggest that the complex can bind to nucleic acids by intercalation via both the dpqc ligand and the long aliphatic chain of the complex into the base pairs of DNA/RNA. In vitro cytotoxicity experiments show that the surfactant cobalt(III) complex exhibits cytotoxic activity against the HepG2 (human hepatocellular liver carcinoma) tumor cell lines and found to be active.