RESUMEN
High levels of triglycerides in blood can harden and block the arteries increasing the risk of heart disease and strokes. Triglycerides are important constituents of oils and fats used in various foods. The triglyceride content in commercial preparations of oils is estimated using conventional methods. In the present study, an electrochemical biosensor with lipase immobilized novel conductive polymer film has been developed for estimating triglyceride content in a variety of products. The portable biosensor can bring down the detection costs dramatically and can be used for varied purposes. It is based on cyclic voltammetry and has a three-electrode configuration system. Glassy carbon electrode is functionalized with nanoparticles embedded in polyethyleneimine and lipase is immobilized using glutaraldehyde. The strategy increases the electrochemical conductance manifold and overcomes the hindrance to lipase posed by membranes as it is oriented on the outside of the membrane. Thus, it increases the sensitivity and selectivity of detection. Results of scanning electron microscopy and FT-IR spectroscopy were used for characterizing the electrode surface. Linear range of the electrode for triglycerides is 100-500 mg/dL. The sensor was used successfully to determine triglyceride content in several real samples and the average recovery values lie from 95.47 % to 101.05 %.
Asunto(s)
Técnicas Biosensibles , Nanopartículas , Triglicéridos , Polímeros , Enzimas Inmovilizadas/química , Lipasa/química , Espectroscopía Infrarroja por Transformada de Fourier , Nanopartículas/química , Técnicas Biosensibles/métodos , Electrodos , Técnicas Electroquímicas , Límite de DetecciónRESUMEN
Use of immobilized acetylcholine esterase (AChE) for detecting organophosphorus pesticides in water sources and body fluids can bring down the detection costs dramatically. In the present study, AChE was directly doped on multiwalled carbon nanotube (MWCNT) surface modified with carboxylic groups through amide bond and used for organophosphorus pesticide detection. Amide bond formation between MWCNTs and the enzyme molecules avoid use of any intermediate membranes, cross-linkers or binding materials. This strategy overcomes the hindrance to electron transfer posed by membranes or cross-linkers and increases the sensitivity of detection. MWCNTs carrying carboxyl groups were deposited on glassy carbon electrode and were subsequently immobilized with AChE. The activity of AChE was monitored by cyclic voltammetry after immobilization. Scanning electron microscopy and atomic force microscopy were used to characterize the electrode surface. FT-IR spectra were taken to characterize enzyme-MWCNT complex. Under optimized parameters, the electrode showed linear range between 10 and 50â¯nM, which is promising for detection of trace amounts of the pesticide. The lower and higher detection limits of the sensor are 0.1â¯nM and 500â¯nM respectively. The stability and reusability of the electrode were determined. Finally, successful detection of organophosphorus compounds in real samples established it as reliable for sensor applications.