RESUMEN

Peptide microarrays are ideal tools for a variety of applications ranging from epitope mapping to immune monitoring. Here we present a method for high-throughput screening of biological samples using only standard microtiter plate equipment. Parallel incubation of a large number of samples with a small library of peptides is enabled by printing multiple identical mini-arrays on one microarray slide and further combining four slides to yield an incubation frame possessing the dimensions of a 96-well microtiter plate. Applying conventional lab equipment such as ELISA washers, hundreds of samples can be processed in 1 day yielding approx. 200 data points in triplicates per sample.

Asunto(s)

Péptidos/metabolismo , Análisis por Matrices de Proteínas/métodos , Secuencia de Aminoácidos , Métodos Analíticos de la Preparación de la Muestra , Humanos , Datos de Secuencia Molecular , Péptidos/química