RESUMEN

BACKGROUND: Siderophores are small-molecule iron-chelators produced by microorganisms and plants growing mostly under low iron conditions. Siderophores allow iron capture and transport through cell membranes into the cytoplasm, where iron is released for use in biological processes. These bacterial iron uptake systems can be used for antibiotic conjugation or as targets for killing pathogenic bacteria. Siderophores have been explored recently because of their potential applications in environmental and therapeutic research. They are present in Streptomyces, Grampositive bacteria that are an important source for discovering new siderophores. OBJECTIVE: This review summarizes siderophore molecules produced by the genus Streptomyces emphasizing their potential as biotechnological producers and also illustrating genomic tools for discovering siderophores useful for treating bacterial infections. METHODS: The literature search was performed using PUBMED and MEDLINE databases with keywords siderophore, secondary metabolites, Trojan horse strategy, sideromycin and Streptomyces. The literature research focused on bibliographic databases including all siderophores identified in the genus Streptomyces. In addition, reference genomes of Streptomyces from GenBank were used to identify siderophore biosynthetic gene clusters by using the antiSMASH platform. RESULTS: This review has highlighted some of the many siderophore molecules produced by Streptomyces, illustrating the diversity of their chemical structures and a wide spectrum of bioactivities against pathogenic bacteria. Furthermore, the possibility of using siderophores conjugated with antibiotics could be an alternative to overcome bacterial resistance to drugs and could improve their therapeutic efficacy. CONCLUSION: This review confirms the importance of Streptomyces as a rich source of siderophores, and underlines their potential as antibacterial agents.

Asunto(s)

Sideróforos , Streptomyces , Antibacterianos/farmacología , Hierro , Quelantes del Hierro

RESUMEN

OBJECTIVE: To examine the association between reflective function and global functionality in borderline personality disorder (BPD) patients, controlling for symptomatology and defensive style. METHOD: Thirty-nine female inpatients were evaluated employing a sociodemographic questionnaire, the Structured Clinical Interview for Personality Disorders-II (SCID-II), the Self-Reporting Questionnaire (SRQ-20), the Reflective Functioning Questionnaire (RFQ), the Defence Style Questionnaire-40 (DSQ-40) and the Global Assessment of Functioning (GAF). RESULTS: Functionality was inversely associated with the reflective function uncertainty score (-.458; p < .01) and neurotic defences (-.335; p < .05). Symptom severity (SRQ-20) was associated with the use of immature defences (-.445; p < .01). The association between functionality and the reflective function uncertainty score remained significant, even when controlled for symptoms and defensive style (p = .002). CONCLUSION: The ability to mentalise seems to play a central and somehow independent role in BPD psychopathology.

Asunto(s)

Trastorno de Personalidad Limítrofe , Femenino , Humanos , Psicopatología

RESUMEN

INTRODUCTION: Eating disorders (EDs) affect up to 13% of young people and are associated with significant morbidity and mortality. Nevertheless, important, internationally recognized instruments for brief ED screening (Sick Control One Stone Fat Food Questionnaire [SCOFF]), symptom severity assessment and diagnosis (Eating Disorder Examination Questionnaire [EDE-Q]) and assessment of ED-associated psychosocial impairment (Clinical Impairment Assessment Questionnaire [CIA]) were not yet available in Brazilian Portuguese. Our objective was to perform the cross-cultural adaptation and translation into Brazilian Portuguese of the instruments SCOFF, EDE-Q and CIA. METHOD: The process involved a series of standardized steps, as well as discussions with experts. First, the relevance and adequacy of the scales' items to our culture and population were extensively discussed. Then, two independent groups translated the original documents, creating versions that were compared. With the participation of external ED experts (i.e., who did not take part in the translation process), synthesized versions were produced. The syntheses were then applied to a focal group of patients with ED (n = 8). After that step, a preliminary version of the three scales in Brazilian Portuguese was produced and sent for back-translation by two English native speakers, who worked independently. A synthesis of the back-translations, along with the preliminary versions in Brazilian Portuguese, were sent to the original authors. RESULTS: The Brazilian Portuguese versions of SCOFF, EDE-Q and CIA were approved by the original authors and are now available for use. CONCLUSION: This study provides important tools for the ED research field in Brazil.

Asunto(s)

Trastornos de Alimentación y de la Ingestión de Alimentos/diagnóstico , Escalas de Valoración Psiquiátrica , Psicometría/métodos , Adulto , Humanos , Psicometría/instrumentación

RESUMEN

BACKGROUND: Over the last few years, fungal infections have emerged as a worrisome global public health problem. Candidiasis is a disease caused by Candida species and has been a problem worldwide mainly for immunosuppressed patients. Lately, the resistant strains and side effects have been reported as important issues for treating Candidiasis, which have to be solved by identifying new drugs. OBJECTIVE: The goal of this work was to synthesize a series of 1,3-benzoxathiol-2-one derivatives, XYbenzo[ d][1,3]oxathiol-2-ones, and evaluate their antifungal activity against five Candida species. METHODS: In vitro antifungal screening test and minimum inhibitory concentration determination were performed according to CLSI protocols using ketoconazole as the reference drug. The cytotoxicity of the most active compounds was evaluated by hemolysis and MTT (Vero cells) assays. RESULTS: Compounds 2 (XY = 6-hydroxy-5-nitro, MIC = 4-32 µg/mL) and 7 (XY = 6-acetoxy-5-nitro, MIC =16-64 µg/mL) showed good results when compared with current antifungals in CLSI values (MIC = 0.04-250 µg/mL). These compounds exhibited a safer cytotoxicity as well as a lower hemolytic profile than ketoconazole. CONCLUSION: Overall, the in vitro results pointed to the potential of compounds 2 and 7 as new antifungal prototypes to be further explored.

Asunto(s)

Antifúngicos/farmacología , Compuestos Heterocíclicos con 2 Anillos/farmacología , Lactonas/farmacología , Antifúngicos/síntesis química , Antifúngicos/química , Antifúngicos/toxicidad , Candida/efectos de los fármacos , Cristalografía por Rayos X , Compuestos Heterocíclicos con 2 Anillos/síntesis química , Compuestos Heterocíclicos con 2 Anillos/química , Compuestos Heterocíclicos con 2 Anillos/toxicidad , Lactonas/síntesis química , Lactonas/química , Lactonas/toxicidad , Pruebas de Sensibilidad Microbiana , Estructura Molecular

RESUMEN

Thrombosis related diseases are among the main causes of death and incapacity in the world. Despite the existence of antithrombotic agents available for therapy, they still present adverse effects like hemorrhagic risks which justify the search for new options. Recently, pachydictyol A, isopachydictyol A, and dichotomanol, three diterpenes isolated from Brazilian marine brown alga Dictyota menstrualis were identified as potent antithrombotic molecules through inhibition of thrombin, a key enzyme of coagulation cascade and a platelet agonist. Due to the biotechnological potential of these marine metabolites, in this work we evaluated their binding mode to thrombin in silico and identified structural features related to the activity in order to characterize their molecular mechanism. According to our theoretical studies including structure-activity relationship and molecular docking analysis, the highest dipole moment, polar surface area, and lowest electronic density of dichotomanol are probably involved in its higher inhibition percentage towards thrombin catalytic activity compared to pachydictyol A and isopachydictyol A. Interestingly, the molecular docking studies also revealed a good shape complementarity of pachydictyol A and isopachydictyol A and interactions with important residues and regions (e.g., H57, S195, W215, G216, and loop-60), which probably justify their thrombin inhibitor effects demonstrated in vitro. Finally, this study explored the structural features and binding mode of these three diterpenes in thrombin which reinforced their potential to be further explored and may help in the design of new antithrombotic agents.

Asunto(s)

Anticoagulantes/química , Anticoagulantes/farmacología , Diterpenos/química , Diterpenos/farmacología , Fibrinolíticos/química , Fibrinolíticos/farmacología , Trombina/antagonistas & inhibidores , Organismos Acuáticos/química , Coagulación Sanguínea/efectos de los fármacos , Brasil , Modelos Moleculares , Simulación del Acoplamiento Molecular/métodos , Phaeophyceae/química , Relación Estructura-Actividad , Trombosis/tratamiento farmacológico

RESUMEN

CYP51 is an enzyme of sterol biosynthesis pathway present in animals, plants, protozoa and fungi. This enzyme is described as an important drug target that is still of interest. Therefore, in this work, we reviewed the structure and function of CYP51 and explored the molecular modeling approaches for the development of new antifungal and antiprotozoans that target this enzyme. Crystallographic structures of CYP51 of some organisms have already been described in the literature, which enable the construction of homology models of other organisms' enzymes and molecular docking studies of new ligands. The binding mode and interactions of some new series of azoles with antifungal or antiprotozoan activities has been studied and showed important residues of the active site. Molecular modeling is an important tool to be explored for the discovery and optimization of CYP51 inhibitors with better activities, pharmacokinetics, and toxicological profiles.

Asunto(s)

Inhibidores de 14 alfa Desmetilasa/farmacología , Antifúngicos/farmacología , Antiprotozoarios/farmacología , Diseño de Fármacos , Simulación del Acoplamiento Molecular , Esterol 14-Desmetilasa/metabolismo , Inhibidores de 14 alfa Desmetilasa/química , Inhibidores de 14 alfa Desmetilasa/toxicidad , Animales , Antifúngicos/química , Antifúngicos/toxicidad , Antiprotozoarios/química , Antiprotozoarios/toxicidad , Sitios de Unión , Humanos , Micosis/tratamiento farmacológico , Micosis/enzimología , Micosis/microbiología , Unión Proteica , Estructura Secundaria de Proteína , Infecciones por Protozoos/tratamiento farmacológico , Infecciones por Protozoos/enzimología , Infecciones por Protozoos/parasitología , Esterol 14-Desmetilasa/biosíntesis , Especificidad por Sustrato

RESUMEN

BACKGROUND: Herpes simplex viruses (HSV) are leading causes of human infections which result in severe manifestations, especially in neonates, immunocompromised and/or transplanted individuals. Current HSV type-1 (HSV-1) resistance to standard antiviral agents is a therapeutic challenge, especially for treating immunocompromised patients. METHODS: Herein we describe the promising antiviral profile of three 2-aminomethyl-3-hydroxy-1,4-naphthoquinones against HSV-1 using Vero cells. RESULTS: The in silico theoretical analysis indicated that the lowest unoccupied molecular orbital (LUMO) and the conformational features of these molecules are important structural features for modulating their biological activity. Our in vitro results showed that these compounds have significant anti-HSV-1 activity comparable to acyclovir, the antiviral currently used clinically. Importantly two of them showed a lower cytotoxicity profile against Vero cells than acyclovir. The inhibitory mechanism analysis using a time-of-addition assay revealed that all compounds inhibit the late phase of lytic replication. Finally, the highest selectivity index of the first tested compound was almost twice as high as that of acyclovir. CONCLUSIONS: Since resistance is still a problem for treating HSV infections, these compounds present a promising profile toward the development of new strategies for anti-HSV-1 therapy.

Asunto(s)

Antivirales/farmacología , Herpesvirus Humano 1/efectos de los fármacos , Naftoquinonas/farmacología , Aciclovir/farmacología , Animales , Antivirales/química , Antivirales/toxicidad , Supervivencia Celular/efectos de los fármacos , Chlorocebus aethiops , Simulación por Computador , Farmacorresistencia Viral , Herpes Simple/tratamiento farmacológico , Herpes Simple/inmunología , Herpes Simple/virología , Humanos , Huésped Inmunocomprometido , Pruebas de Sensibilidad Microbiana , Naftoquinonas/química , Naftoquinonas/toxicidad , Células Vero , Ensayo de Placa Viral

RESUMEN

Numerous factors contribute to perinatal risk, many of which remain undefined. This study sought to determine the frequency of fish intake in postpartum women, and to establish a relationship between the rates of immunoreactivity for antigens from Anisakis spp. and high-risk pregnancy. In this prospective noninterventional study, a structured questionnaire was administered and serum was collected from postpartum women at two perinatal centers (a high-risk birth unit [HRBU] and a low-risk birth unit [LRBU]) in the Niteroi municipality of Brazil. Anisakis species-specific IgG and IgE were measured by ELISA. The chisquared test was performed, and odds ratios (ORs) with their 95% confidence intervals were estimated. The t-test or Mann-Whitney test was applied to continuous, normally distributed variables. In total, 309 women (170 from HRBU, 139 from LRBU) between 24.8 and 26.7 years old with a median of 6 to 8 prenatal visits were enrolled. Women in the two units exhibited differences in some variables, including prenatal care (p = 0.01), maternal and fetal risk (p = 0.00; OR = 6.17), and gestational age (p = 0.00), but no differences in fish consumption (p = 0.29), frequency of fish intake (p = 0.40), allergic symptoms (p = 0.51), or frequency of anti-Anisakis reactivity (p = 0.22). Logistic regression analysis revealed that only age was independently associated with postpartum anti-Anisakis reactivity. This study confirmed a low prevalence of fish intake and suggested that Anisakis spp. had no impact on high-risk pregnancies among this postpartum study population.

Asunto(s)

Anisakiasis/diagnóstico , Anisakis/aislamiento & purificación , Antígenos Helmínticos/sangre , Complicaciones Parasitarias del Embarazo/diagnóstico , Embarazo de Alto Riesgo , Animales , Anisakiasis/epidemiología , Anisakiasis/parasitología , Anisakis/inmunología , Brasil/epidemiología , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Inmunoglobulina E/sangre , Inmunoglobulina G/sangre , Embarazo , Complicaciones Parasitarias del Embarazo/parasitología , Prevalencia , Estudios Prospectivos , Encuestas y Cuestionarios

RESUMEN

This paper aims to estimate the temperature equivalent to 10% (T10%), 50% (T50%) and 90% (T90%) of distilled volume in crude oils using (1)H NMR and support vector regression (SVR). Confidence intervals for the predicted values were calculated using a boosting-type ensemble method in a procedure called ensemble support vector regression (eSVR). The estimated confidence intervals obtained by eSVR were compared with previously accepted calculations from partial least squares (PLS) models and a boosting-type ensemble applied in the PLS method (ePLS). By using the proposed boosting strategy, it was possible to identify outliers in the T10% property dataset. The eSVR procedure improved the accuracy of the distillation temperature predictions in relation to standard PLS, ePLS and SVR. For T10%, a root mean square error of prediction (RMSEP) of 11.6°C was obtained in comparison with 15.6°C for PLS, 15.1°C for ePLS and 28.4°C for SVR. The RMSEPs for T50% were 24.2°C, 23.4°C, 22.8°C and 14.4°C for PLS, ePLS, SVR and eSVR, respectively. For T90%, the values of RMSEP were 39.0°C, 39.9°C and 39.9°C for PLS, ePLS, SVR and eSVR, respectively. The confidence intervals calculated by the proposed boosting methodology presented acceptable values for the three properties analyzed; however, they were lower than those calculated by the standard methodology for PLS.

RESUMEN

Negative-ion mode electrospray ionization, ESI(-), with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) was coupled to a Partial Least Squares (PLS) regression and variable selection methods to estimate the total acid number (TAN) of Brazilian crude oil samples. Generally, ESI(-)-FT-ICR mass spectra present a power of resolution of ca. 500,000 and a mass accuracy less than 1 ppm, producing a data matrix containing over 5700 variables per sample. These variables correspond to heteroatom-containing species detected as deprotonated molecules, [M - H](-) ions, which are identified primarily as naphthenic acids, phenols and carbazole analog species. The TAN values for all samples ranged from 0.06 to 3.61 mg of KOH g(-1). To facilitate the spectral interpretation, three methods of variable selection were studied: variable importance in the projection (VIP), interval partial least squares (iPLS) and elimination of uninformative variables (UVE). The UVE method seems to be more appropriate for selecting important variables, reducing the dimension of the variables to 183 and producing a root mean square error of prediction of 0.32 mg of KOH g(-1). By reducing the size of the data, it was possible to relate the selected variables with their corresponding molecular formulas, thus identifying the main chemical species responsible for the TAN values.

RESUMEN

Este relato de caso propõe-se apresentar o atendimento de uma criança decinco anos de idade que, no decorrer de suas sessões de psicoterapia, pôdecomunicar à terapeuta o abuso sexual que vinha sofrendo. Para a compreensãodinâmica do caso, faz-se uma breve revisão da literatura sobre conflitivaedípica, abuso sexual e contratransferência. Paciente e terapeuta podem encontraruma saída quando ambos se opõem ao abuso; não havendo, dessaforma, meios para manter o trauma “esquecido”.

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This case report presents the psychotherapeutic work of a 5-year-old boy who,as the sessions developed, was able to report the sexual abuse he had beensubject to his therapist. To understand the case dynamics, a brief literaturereview on Oedipus conflict, sexual abuse and countertransference is presented.Patient and therapist can find a way out when both oppose the abuse in orderthat by no means the trauma is kept “forgotten.”.

Asunto(s)

Preescolar , Niño , Psicoanálisis

RESUMEN

Este relato de caso propõe-se apresentar o atendimento de uma criança decinco anos de idade que, no decorrer de suas sessões de psicoterapia, pôdecomunicar à terapeuta o abuso sexual que vinha sofrendo. Para a compreensãodinâmica do caso, faz-se uma breve revisão da literatura sobre conflitivaedípica, abuso sexual e contratransferência. Paciente e terapeuta podem encontraruma saída quando ambos se opõem ao abuso; não havendo, dessaforma, meios para manter o trauma “esquecido”.(AU)

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This case report presents the psychotherapeutic work of a 5-year-old boy who,as the sessions developed, was able to report the sexual abuse he had beensubject to his therapist. To understand the case dynamics, a brief literaturereview on Oedipus conflict, sexual abuse and countertransference is presented.Patient and therapist can find a way out when both oppose the abuse in orderthat by no means the trauma is kept “forgotten.”(AU)

Asunto(s)

Preescolar , Niño , Psicoanálisis

RESUMEN

OBJECTIVE: The Parental Bonding Instrument (PBI) is a widely used measure of parenting. Recent studies have proposed different factor structures. There is a disagreement in the literature about whether the PBI is best used as a two-factor or a three-factor measure. METHOD: Two hundred and fifty-seven female adults were recruited from a clinical population (139 psychiatric patients and 118 controls) and were requested to complete the PBI. Maximum likelihood confirmatory factor analyses were performed to compare the five different factor structures in terms of model fit. RESULTS: The poorest fit to the data was obtained by the Parker et al. model. The Kendler model was the only model that had an adjusted goodness-of-fit index >0.8 regarding both paternal and maternal PBI. When considering invariance of factor structure across age subgroups, the Kendler model was also the only acceptable model. CONCLUSION: Three-factor structures are preferable to two-factor structures. The Kendler model was the only one to provide an acceptable fit, but it must be considered that it was a female sample, and when considering gender subgroups other studies have found the same results. Despite the gender limitation, the present study contributes to a better understanding and use of the PBI in Brazilian samples.

Asunto(s)

Relaciones Madre-Hijo , Apego a Objetos , Responsabilidad Parental , Encuestas y Cuestionarios , Adulto , Brasil/epidemiología , Análisis Factorial , Femenino , Humanos , Trastornos Mentales/diagnóstico , Trastornos Mentales/epidemiología , Trastornos Mentales/psicología , Prevalencia , Índice de Severidad de la Enfermedad

RESUMEN

The study aims to compare the mental states and countertransference responses of 92 psychodynamically oriented psychotherapists, male and female, experienced and inexperienced, facing written reports of real patients who experienced traumatic events. Two vignettes were presented: one of a sexual violence, the other the sudden death of a significant person. The Mental States Rating System (MSRS; Bouchard, Picard, Audet, Brisson, & Carrier, 1998), the MSRS Self-Report (Goldfeld & Bouchard, 2004), and the Inventory of Countertransference Behavior (ICB; Friedman & Gelso, 2000) were used. Results showed that the mourning vignette led to more reflective responses (MSRS) and the rape case was associated with more negative countertransference reactions (ICB). Female participants were more reflective (MSRS); male therapists used less mentalized states (MSRS Self-Report) and expressed more negative reactions (ICB) for both scenarios. Experienced therapists showed more positive reactions on the ICB. The construct validity of the instruments is discussed in relation to the findings.

Asunto(s)

Contratransferencia , Pesar , Escala del Estado Mental , Terapia Psicoanalítica , Violación/psicología , Trastornos por Estrés Postraumático/psicología , Violencia/psicología , Adulto , Concienciación , Femenino , Humanos , Masculino , Persona de Mediana Edad , Relaciones Profesional-Paciente , Factores Sexuales

RESUMEN

INTRODUÇÃO: O estabelecimento de uma aliança terapêutica de boa qualidade em uma psicoterapia psicanalítica é fundamental para o processo terapêutico. Este estudo avaliou a influência do nível de funcionamento defensivo do paciente na qualidade da aliança terapêutica estabelecida durante a psicoterapia. MÉTODO: Para avaliação da qualidade da aliança estabelecida, pacientes em psicoterapia psicanalítica e seus respectivos terapeutas responderam ao Helping Alliance Questionnaire (versão paciente e versão terapeuta, respectivamente). O nível defensivo foi inferido através da Escala de Funcionamento Defensivo proposta no Manual de Diagnóstico e Estatística das Perturbações Mentais, quarta edição, texto revisado. RESULTADOS: Não houve associação entre o estabelecimento de uma aliança terapêutica de boa qualidade e o nível defensivo do paciente. No entanto, houve diferença significativa quando a versão do terapeuta foi comparada com a respondida pelo paciente: os pacientes estabeleceram uma aliança terapêutica de melhor qualidade em relação a seus terapeutas do que o inverso. CONCLUSÕES: O fato de a aliança terapêutica de boa qualidade ter se estabelecido independentemente do nível defensivo do paciente sugere que o treinamento e as características pessoais do terapeuta podem levar a uma capacidade de conectar-se com o paciente, apesar do grau de comprometimento do seu funcionamento psíquico.

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BACKGROUND: The quality of a therapeutic alliance is essential in psychoanalytic psychotherapy and influences the therapeutic process. This study evaluated the relationship between the level of defense mechanisms and the quality of therapeutic alliance established during psychotherapy. METHOD: Patients in psychotherapy and their respective therapists completed the Helping Alliance Questionnaire (patient version and therapist version, respectively). The level of defenses was inferred by the Defensive Functioning Scale proposed in the Diagnostic and Statistical Manual of Mental Disorders, fourth edition, text revision. RESULTS: There was no association between the quality of therapeutic alliance and the patient's level of defense mechanisms in this sample. On the other hand, there was a difference when the therapist version was compared to the patient version: patients established a stronger therapeutic alliance in relation to their therapists. CONCLUSIONS: The lack of influence of defense level in the quality of therapeutic alliance suggests that the therapist's training and personal characteristics may lead to the ability of connecting with the patient, despite impairment in their psychic functioning.

RESUMEN

INTRODUÇÃO: O artigo apresenta a adaptação transcultural do Mental States Rating System, uma escala de análise de conteúdo do discurso, seja ele falado, descrito ou filmado, que abrange de modo amplo tipos de contratransferência. MÉTODO: Foram realizadas as etapas de equivalência conceitual, equivalência de itens, equivalência semântica, equivalência operacional, equivalência funcional e aprovação da versão final pelo autor original do instrumento. RESULTADOS: Os critérios de equivalência foram satisfeitos, tendo a versão final sido aprovada pelo autor do instrumento original. CONCLUSÃO: A adaptação do Mental States Rating System disponibiliza para uso um instrumento que não só abrange todas as categorias de contratransferência descritas na literatura, mas as amplia, através da Teoria dos Estados Mentais. Constitui, desse modo, uma ferramenta de grande utilidade para pesquisa em psicoterapia e psicanálise, onde a contratransferência tem se mostrado um importante recurso, tornando-se ainda mais crucial nas patologias fundamentadas em estágios precoces do desenvolvimento, nos casos graves e nos traumas severos.

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INTRODUCTION: This article presents a cross-cultural adaptation of the Mental States Rating System, a content analysis scale applied to spoken, written or taped material, which covers a wide range of countertransference categories. METHOD: The following steps were performed: conceptual equivalence, item equivalence, semantic equivalence, operational equivalence, functional equivalence, and approval of the final version by the author of the original instrument. RESULTS: The study has reached the objectives of equivalence, and the final Brazilian Portuguese version has been approved by the original author. CONCLUSION: The study provides a Brazilian Portuguese version of an instrument that not only covers all the range of categories encompassed by countertransference described in the literature, but also expands it through the Mental States Theory. This represents a valuable tool for research on psychotherapy and psychoanalysis, where countertransference has proved to be an important resource, especially for the treatment of diseases based on early stages of development, for severe mental diseases and severe trauma.

RESUMEN

OBJETIVO: O artigo apresenta a adaptação transcultural do Inventory of Countertransference Behavior (ICB) para o português brasileiro. O ICB constitui-se numa escala de 21 itens que busca acessar o comportamento contratransferencial. Esta escala, que deve ser preenchida pelo supervisor após a sessão de supervisão, abrange a contratransferência em suas categorias positiva e negativa. MÉTODO: Foram realizadas as etapas de equivalência conceitual, equivalência de itens, equivalência semântica, equivalência operacional, equivalência funcional e aprovação da versão final pelo autor original do instrumento. RESULTADOS: Os critérios de equivalência foram satisfeitos, tendo a versão final sido aprovada pelo autor do instrumento original. CONCLUSÃO: A adaptação do ICB disponibiliza para uso um instrumento de utilização prática, que abrange as categorias de contratransferência positiva e negativa. Constitui, deste modo, uma ferramenta de grande utilidade para a clínica, a supervisão e a pesquisa em psicoterapia e psicanálise, onde a contratransferência tem se mostrado um importante recurso, especialmente no tratamento de patologias fundamentadas em estágios precoces do desenvolvimento, nos casos graves e nos traumas severos.

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OBJECTIVE: This article presents a cross-cultural adaptation of the Inventory of Countertransference Behavior into Brazilian Portuguese. The Inventory of Countertransference Behavior is a 21-item scale designed to assess countertransferential behavior. This scale, which should be completed by the supervisor after a supervised session, comprehends countertransference and its positive and negative categories. METHOD: The following steps were performed: conceptual equivalence, item equivalence, semantic equivalence, operational equivalence, functional equivalence, and approval of the final version by the author of the original instrument. RESULTS: The study reached the objectives of equivalence, and the final Brazilian Portuguese version was approved by the original author. CONCLUSION: This adaptation provides a Brazilian Portuguese version of a practical instrument to assess positive and negative countertransference. It represents a valuable instrument for therapists, supervisors and researchers on psychotherapy and psychoanalysis, where countertransference has proved to be an important resource, especially to the treatment of diseases based on early stages of development, as well as to severe cases and severe trauma.

Asunto(s)

Humanos , Masculino , Femenino , Contratransferencia , Pesos y Medidas , Psicoanálisis/instrumentación , Psicoanálisis/métodos , Psicoterapia/instrumentación , Psicoterapia/métodos , Evaluación de Programas e Instrumentos de Investigación , Investigación

RESUMEN

Com a comprovação da eficácia de diferentes modelos de psicoterapia para o tratamento dos distúrbios psiquiátricos, ferramentas de pesquisa que viabilizem a investigação das especificidades dos diferentes métodos são essenciais para determinar qual tratamento é o mais adequado a um paciente em particular. Método: A partir da revisão da literatura, da discussão com experts, da realização de grupos focais e das aplicações iniciais, foi elaborada uma Versão Piloto do Instrumento de Avaliação para Psicoterapia Psicanalítica (IAPP-VP), utilizada durante o processo de avaliação de pacientes para psicoterapia psicanalítica. Foram realizadas análises fatoriais sucessivas que auxiliaram na elaboração de um modelo teórico confirmado por meio da modelagem de equação estrutural, resultando no IAIPP. Objetivos: Desenvolver e testar o IAIPP-VP, elaborando um modelo teórico para embasar a elaboração da versão final do instrumento. Resultados: O IAIPP-VP mostrou-se bastante útil na avaliação, planejamento terapêutico e supervisão dos casos. A análise dos dados resultou em um modelo em que oito itens compõem duas dimensões principais (nível de adaptação e motivação para tratamento psicanalítico), que resultariam na capacidade de formar uma aliança de qualidade; logo, em uma maior probabilidade de desfecho positivo em psicoterapia psicanalítica. A versão final do IAIPP foi um index com onze itens, com respostas tipo likert 0 a 9. Discussão: A elaboração desse tipo de ferramenta é um importante meio de qualificar pesquisa, ensino e assistência em psicoterapia psicanalítica. Esse modelo inicial deve servir de base para estudos subseqüentes que venham a confirmar, ou não, esses achados.

Asunto(s)

Evaluación de Programas e Instrumentos de Investigación , Estudio de Evaluación , Análisis Factorial , Modelos Psicológicos , Psicoanálisis , Psicoterapia

RESUMEN

Com a comprovação da eficácia de diferentes modelos de psicoterapia para o tratamento dos distúrbios psiquiátricos, ferramentas de pesquisa que viabilizem a investigação das especificidades dos diferentes métodos são essenciais para determinar qual tratamento é o mais adequado a um paciente em particular. Método: A partir da revisão da literatura, da discussão com experts, da realização de grupos focais e das aplicações iniciais, foi elaborada uma Versão Piloto do Instrumento de Avaliação para Psicoterapia Psicanalítica (IAPP-VP), utilizada durante o processo de avaliação de pacientes para psicoterapia psicanalítica. Foram realizadas análises fatoriais sucessivas que auxiliaram na elaboração de um modelo teórico confirmado por meio da modelagem de equação estrutural, resultando no IAIPP. Objetivos: Desenvolver e testar o IAIPP-VP, elaborando um modelo teórico para embasar a elaboração da versão final do instrumento. Resultados: O IAIPP-VP mostrou-se bastante útil na avaliação, planejamento terapêutico e supervisão dos casos. A análise dos dados resultou em um modelo em que oito itens compõem duas dimensões principais (nível de adaptação e motivação para tratamento psicanalítico), que resultariam na capacidade de formar uma aliança de qualidade; logo, em uma maior probabilidade de desfecho positivo em psicoterapia psicanalítica. A versão final do IAIPP foi um index com onze itens, com respostas tipo likert 0 a 9. Discussão: A elaboração desse tipo de ferramenta é um importante meio de qualificar pesquisa, ensino e assistência em psicoterapia psicanalítica. Esse modelo inicial deve servir de base para estudos subseqüentes que venham a confirmar, ou não, esses achados.(AU)

Asunto(s)

Psicoterapia , Psicoanálisis , Evaluación de Programas e Instrumentos de Investigación , Estudio de Evaluación , Análisis Factorial , Modelos Psicológicos

RESUMEN

INTRODUÇÃO: O desenvolvimento da versão em português do Brasil do Beziehungs-Muster Fragebogen, conhecido na literatura internacional como Relationship Patterns Questionnaire, questionário que avalia o padrão central de relação, visa à introdução, em nosso meio, de uma medida auto-aplicável para implementação de projetos de pesquisa cuja questão envolva o construto transferência. METODOLOGIA: As etapas para o desenvolvimento da versão foram: obtenção da licença dos autores; tradução do instrumento original para o português do Brasil; julgamento e ajuste do material traduzido por profissionais da área da psiquiatria e psicologia; retrotradução; julgamento da equivalência semântica; consenso de profissionais da área da psiquiatria e psicologia sobre a adequação do instrumento à nossa cultura; interlocução com a população-alvo. CONCLUSÃO: O Beziehungs-Muster Fragebogen é uma medida auto-aplicável, para avaliação da transferência ou padrão central da relação, que poderá facilitar a implementação de projetos para investigar os aspectos da relação terapêutica adjuntos à transferência. É um método de fácil aplicação e análise, baixo custo e que dispensa o uso de vídeos ou gravadores na sessão. Estudos investigatórios acerca do padrão de relação poderão fornecer mais resultados sobre a adaptabilidade desse instrumento à nossa cultura.

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INTRODUCTION: The Beziehungs-Muster Fragebogen (BeMus-3), known in the international literature as the Relationship Patterns Questionnaire (RPQ), is a questionnaire that evaluates the central pattern of relationship. The development of its Brazilian Portuguese version aims at introducing, in our country, a self-report measurement to implement research projects involving the construct of transference. METHODOLOGY: The development stages of this version were: obtaining permission from the authors; translation of the original instrument into Brazilian Portuguese; evaluation and adjustment of the translated material by psychiatry and psychology professionals; back-translation; evaluation of semantic equivalence; consensus of psychiatry and psychology professionals on the adequacy of the instrument to our culture; interchange with the target population. CONCLUSION: BeMus-3 is a self-report measurement to assess the transference or central pattern of relationship that can facilitate the implementation of projects to investigate the transference-related aspects of therapeutic relationship. This method is easy to apply and analyze, has a low cost and does not require the use of video or voice recorders during the session. Investigative studies on the pattern of relationship may provide further results on the adaptability of this instrument to our culture.