RESUMEN
A simple leak-free micropore chamber containing protozoan parasite species was implanted subcutaneously on the back of hamsters and evaluated for viability and multiplication of protozoan parasites. Trophozoites of defined strains of Entamoeba histolytica, Giardia lamblia, Trichomonas vaginalis, and Tritrichomonas foetus were used; their survival and multiplication in the chambers formed the basis of evaluation. Entamoeba histolytica and G. lamblia did not survive more than 6 hr and succumbed due to cellular adhesion. Trichomonas vaginalis and T. foetus survived 3 and 6 days and multiplied a maximum of 3.6 and 26 times, respectively. This indicated that exchange of body fluids and cells needed for the survival and multiplication of trichomonads readily occurs. This preliminary observation showed that micropore chambers may be useful for chemotherapeutic and immunological studies on trichomonads in ectopic sites.
Asunto(s)
Cámaras de Difusión de Cultivos/métodos , Trichomonas/crecimiento & desarrollo , Tritrichomonas/crecimiento & desarrollo , Animales , Cricetinae , Entamoeba histolytica/crecimiento & desarrollo , Giardia/crecimiento & desarrollo , Masculino , Filtros MicroporosRESUMEN
A method is described for successfully establishing caecal amoebiasis in hamsters which were not fed and which were pretreated with 1 ml of magnesium sulphate every 24 hours for three days and then given 12 x 10(5) trophozoites of the HM-1 axenic strain or 18 x 10(5) trophozoites of the HK-9 axenic strain of Entamoeba histolytica by the oral route. All the animals developed diarrhoea within 24 hours of infection. When the animals were killed on the fifth day after infection the caecum was swollen and fused. Large macroscopic ulcers full of pus could be seen in the caecum. None of the control animals showed any of the changes mentioned above.
Asunto(s)
Enfermedades del Ciego/etiología , Susceptibilidad a Enfermedades , Disentería Amebiana/etiología , Administración Oral , Animales , Enfermedades del Ciego/patología , Cricetinae , Modelos Animales de Enfermedad , Disentería Amebiana/patología , Entamoeba histolytica , Femenino , MasculinoRESUMEN
In the present study the comparative in-vitro and in-vivo efficacy of Go 10213 was compared with those of metronidazole, secnidazole, tinidazole, ornidazole and nimorazole against a metronidazole-resistant strain of T. vaginalis. Go 10213 was found to be superior in activity. It appears more promising than the drugs mentioned above on the basis of the evidence presented.
Asunto(s)
Antitricomonas/farmacología , Metronidazol/farmacología , Nitroimidazoles/farmacología , Trichomonas vaginalis/efectos de los fármacos , Animales , Farmacorresistencia Microbiana , RatonesRESUMEN
CG 10213-Go, a 5-nitroimidazole derivative synthesized at the CIBA-Geigy Research Centre, Bombay, proved superior to such currently used 5-nitroimidazole derivatives as metronidazole, ornidazole, secnidazole, tinidazole and nimorazole, against experimental amoebic infections of the liver and caecum in a golden hamster model. CG 10213-Go is now undergoing clinical trials.