RESUMEN
Immunoglobulin penicillinase conjugate for the detection of Fr. tularensis antigen in enzyme immunoassay has been prepared. Its sensitivity is 0.7-1.0 ng/ml of Fr. tularensis lipopolysaccharide. The technique for the preparation of this conjugate is simpler than for that of peroxidase conjugate.
Asunto(s)
Antígenos Bacterianos/análisis , Francisella tularensis/inmunología , Haptenos , Inmunoglobulinas , Penicilinasa , Animales , Técnicas para Inmunoenzimas , Kazajstán , Pruebas de Neutralización , Garrapatas/inmunologíaAsunto(s)
Fiebre/microbiología , Infecciones por Pasteurella/microbiología , Pasteurella/aislamiento & purificación , Sepsis/microbiología , Adolescente , Pruebas de Aglutinación/métodos , Femenino , Fiebre/sangre , Fiebre/diagnóstico , Humanos , Infecciones por Pasteurella/sangre , Infecciones por Pasteurella/diagnóstico , Sepsis/sangre , Sepsis/diagnósticoRESUMEN
Industrial production of plague monoclonal antibodies in mice BALB/c was shown possible. Precipitation of the immunoglobulins with caprylic acid provided the highest purity of the preparations. Maintenance of the specific activity by the monoclonal antibodies was stable. The immunoenzymatic conjugate and erythrocytic diagnosticum on the basis of the monoclonal antibodies are sensitive, specific and may be used in antiplague practice.
Asunto(s)
Anticuerpos Monoclonales , Yersinia pestis/inmunología , Animales , Anticuerpos Monoclonales/aislamiento & purificación , Hibridomas/inmunología , Ratones , Ratones Endogámicos BALB CRESUMEN
In the urine of plague-infected great gerbils Yersinia pestis capsular antigen was detected by means of diagnostic preparations, both commercial and experimental (based on monoclonal antibodies). The antigen was detected in many urine samples taken from the animals over a prolonged period. The incidence and duration of antigenuria were found to be related to the survival time of great gerbils after infection and the level of antibodies in their blood. The number of animals with antigenuria markedly exceeded the number of animals from which Y. pestis was isolated, especially at a later period after infection. Examinations of urine samples from live great gerbils trapped in natural foci appears to be a method more effective in epizootiological survey than the bacteriological analysis of the animals.
Asunto(s)
Antígenos Bacterianos/orina , Gerbillinae/inmunología , Peste/inmunología , Yersinia pestis/inmunología , Animales , Anticuerpos Antibacterianos/análisis , Asia Central , Vectores de Enfermedades , Pruebas de Inhibición de Hemaglutinación , Pruebas de Hemaglutinación , Técnicas para Inmunoenzimas , Kazajstán , Pruebas de Neutralización , Peste/microbiología , Peste/mortalidad , Factores de Tiempo , Yersinia pestis/aislamiento & purificaciónRESUMEN
Beta-lactamase (penicillinase) has been used as a marker of monoclonal antibodies in the enzyme immunoassay made with a view to the detection of Y. pestis capsular antigen and antibodies to it. The trial of the enzyme immunoassay with the use of the beta-lactamase conjugate in laboratories and under field conditions has revealed the advantage of this assay over hemagglutination tests commonly used for this purpose.
Asunto(s)
Anticuerpos Monoclonales/análisis , Antígenos Bacterianos/inmunología , Yersinia pestis/inmunología , beta-Lactamasas , Animales , Animales Salvajes , Anticuerpos Antibacterianos/análisis , Bacillus/enzimología , Estudios de Evaluación como Asunto , Pruebas de Hemaglutinación , Técnicas para Inmunoenzimas , Ratones , Ratones Endogámicos BALB C , Pruebas de Neutralización , Peste/diagnóstico , Peste/veterinariaRESUMEN
Monoclonal antibodies to Yersinia pestis capsular antigen were fixed onto the surface of formulated sheep red blood cells. The preparation thus obtained was compared with commercial antibody erythrocyte diagnosticum in the passive hemagglutination test aimed at the search for the capsular antigen in the suspensions of Yersinia pestis museum cultures and in the antigen neutralization test aimed at the search for antibodies in the sera of wild and laboratory animals having had plague. Monoclonal erythrocyte diagnosticum proved to be suitable for the detection of both the capsular antigen and antibodies. The comparison of the results of the passive hemagglutination test and the enzyme immunoassay demonstrated the presence of very close relationship between them.
Asunto(s)
Anticuerpos Antibacterianos/aislamiento & purificación , Anticuerpos Monoclonales/inmunología , Eritrocitos/inmunología , Peste/diagnóstico , Yersinia pestis/inmunología , Animales , Anticuerpos Antibacterianos/inmunología , Anticuerpos Monoclonales/aislamiento & purificación , Antígenos Bacterianos/inmunología , Líquido Ascítico/inmunología , Pruebas de Hemaglutinación , Inmunización/métodos , Técnicas para Inmunoenzimas , Ratones , Ratones Endogámicos BALB CRESUMEN
By the character of the sensitivity of UV-irradiation, to N-methyl-N'-nitro-N-nitrosoguanidine, 5-bromuracil, mitomycin C, crystalviolet, and by the capacity to restore phage injuries the 1435-A and 1435-24 mutants were referred to the uvr-hcr-, 17 mutant--to the uvr-hcr+, and 35 mutant--to lon genotype. As a result of UV irradiation the experimental strains formed heteromorphic forms of bacteria, spindle-shaped, filamentous cells, were sensitive to the action of static electrical field of high frequency, this pointing to disturbance of cellular membranes structure in these mutants.
Asunto(s)
Reparación del ADN , Yersinia pestis , Antibacterianos/farmacología , Bacteriófagos , Bromouracilo/farmacología , Membrana Celular/efectos de los fármacos , Membrana Celular/efectos de la radiación , Violeta de Genciana/farmacología , Mitomicinas/farmacología , Mutación , Nitrosoguanidinas/farmacología , Rayos Ultravioleta , Yersinia pestis/efectos de los fármacos , Yersinia pestis/efectos de la radiaciónRESUMEN
The mutants of plague bacteria deficient in dark repair are described. They are sensitive to UV, mitomycin C, polymixin. Mutants have lost the ability to host cell reactivation in phage T7. The data obtained have shown that the addition of caffeine to the plating medium used to assay post-irradiation viability of UV-sensitive mutants did not influence the survival. Data are also given concerning virulence determinants, biochemical and other properties. The mutants formed P- colonies on hemine medium and Ca+ cells on Higuchi-Smith medium. They are avirulent for albino mice.