RESUMEN
To investigate the effect of tert-butylhydroquinone (tBHQ) on scrotal heat-induced damage in mice testes, 8-week-old mice were divided into 6 groups and administered with or without tBHQ through diet (10 mg/g), intraperitoneal injection (100 mg/kg body weight), or intratestis injection (12.5 mg/kg body weight), respectively. After single scrotal heat exposure (42 °C for 25 min), trunk blood and testes were collected 48 h later. The testes from diet and intraperitoneal tBHQ-treated mice showed more compact interstitial cells and less germ cell loss in the seminiferous epithelium compared with their corresponding non-tBHQ groups. However, intratestis tBHQ treatment showed no marked difference relative to the non-treatment group. In addition, pre-treatment of tBHQ caused lower testosterone concentrations and reduced expression of cytochrome P450 17α-hydroxylase/17,20-lyase (CYP 17) compared to the corresponding non-tBHQ groups. The results indicated that scrotal heat-induced structural damage was partly prevented by pre-treatment of tBHQ, which could be used as an effective antioxidant for preventing scrotal heat-mediated male infertility.
Asunto(s)
Antioxidantes/farmacología , Calor/efectos adversos , Hidroquinonas/farmacología , Infertilidad Masculina/prevención & control , Testículo/efectos de los fármacos , Animales , Antioxidantes/uso terapéutico , Hidroquinonas/uso terapéutico , Infertilidad Masculina/etiología , Masculino , Ratones , Espermatozoides/efectos de los fármacos , Esteroide 17-alfa-Hidroxilasa/genética , Esteroide 17-alfa-Hidroxilasa/metabolismo , Testículo/metabolismo , Testículo/patología , Testosterona/sangreRESUMEN
To determine if Butea superba Roxb., a traditional Thai male potency herb, has androgenic activity in 60-day-old male Wistar rats, we measured its effects on the pituitary-testicular axis and sex organs. Intact and orchidectomized adult male rats were subdivided into five groups (10 rats/group): distilled water, Butea superba (BS)-10, BS-50, BS-250, and testosterone propionate (TP). They received 0, 10, 50, and 250 mg·kg body weight-1·day-1 BS in distilled water by gavage and 6 mg·kg body weight-1·day-1 TP sc, respectively, during the 30-day treatment period. Blood was collected every 15 days and luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone were measured. Changes of weight and histological appearance of sex organs were determined at the end of the 30-day treatment and 15-day post-treatment periods. TP treatment reduced serum FSH and LH levels and significantly increased the weight of the seminal vesicles and epididymis, in accordance with histopathological changes, in both intact and orchidectomized rats. No changes in serum testosterone, LH, and FSH levels were observed in any of the intact rats treated with BS, but a significant increase in seminal vesicle weight was observed only in the BS-250 group. Although a significant reduction in serum LH was detected in the BS-50 and BS-250 groups of orchidectomized rats, no significant change in weight or histology of sex organs was observed. Thus, we conclude that B. superba needs endogenous testosterone to work synergistically to stimulate the accessory sex organ of intact animals and can potentially exhibit an LH reduction effect in orchidectomized animals.
Asunto(s)
Animales , Masculino , Ratas , Butea/química , Hormona Folículo Estimulante/sangre , Hormona Luteinizante/sangre , Extractos Vegetales/farmacología , Testosterona/sangre , Hormona Luteinizante/efectos de los fármacos , Orquiectomía , Tamaño de los Órganos/efectos de los fármacos , Hipófisis/efectos de los fármacos , Radioinmunoensayo , Ratas Wistar , Vesículas Seminales/efectos de los fármacos , Testículo/efectos de los fármacos , Propionato de Testosterona/farmacologíaRESUMEN
To determine if Butea superba Roxb., a traditional Thai male potency herb, has androgenic activity in 60-day-old male Wistar rats, we measured its effects on the pituitary-testicular axis and sex organs. Intact and orchidectomized adult male rats were subdivided into five groups (10 rats/group): distilled water, Butea superba (BS)-10, BS-50, BS-250, and testosterone propionate (TP). They received 0, 10, 50, and 250 mg·kg body weight(-1)·day(-1) BS in distilled water by gavage and 6 mg·kg body weight(-1)·day(-1) TP sc, respectively, during the 30-day treatment period. Blood was collected every 15 days and luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone were measured. Changes of weight and histological appearance of sex organs were determined at the end of the 30-day treatment and 15-day post-treatment periods. TP treatment reduced serum FSH and LH levels and significantly increased the weight of the seminal vesicles and epididymis, in accordance with histopathological changes, in both intact and orchidectomized rats. No changes in serum testosterone, LH, and FSH levels were observed in any of the intact rats treated with BS, but a significant increase in seminal vesicle weight was observed only in the BS-250 group. Although a significant reduction in serum LH was detected in the BS-50 and BS-250 groups of orchidectomized rats, no significant change in weight or histology of sex organs was observed. Thus, we conclude that B. superba needs endogenous testosterone to work synergistically to stimulate the accessory sex organ of intact animals and can potentially exhibit an LH reduction effect in orchidectomized animals.
Asunto(s)
Butea/química , Hormona Folículo Estimulante/sangre , Hormona Luteinizante/sangre , Extractos Vegetales/farmacología , Testosterona/sangre , Animales , Hormona Luteinizante/efectos de los fármacos , Masculino , Orquiectomía , Tamaño de los Órganos/efectos de los fármacos , Hipófisis/efectos de los fármacos , Radioinmunoensayo , Ratas , Ratas Wistar , Vesículas Seminales/efectos de los fármacos , Testículo/efectos de los fármacos , Propionato de Testosterona/farmacologíaRESUMEN
A 20-month-old boy was investigated for persistent liver dysfunction. Liver histologic findings showed chronic hepatitis. The presence of human herpesvirus-6 DNA in liver tissue was demonstrated both by in situ hybridization and by polymerase chain reaction. Human herpesvirus-6 may be a causative agent of chronic hepatitis in this case.
Asunto(s)
ADN Viral/análisis , Exantema Súbito/complicaciones , Hepatitis Viral Humana/virología , Herpesvirus Humano 6/genética , Leucocitos Mononucleares/virología , Biopsia , Southern Blotting , Enfermedad Crónica , Diagnóstico Diferencial , Hepatitis Viral Humana/sangre , Hepatitis Viral Humana/patología , Humanos , Hibridación in Situ , Lactante , Masculino , Reacción en Cadena de la PolimerasaRESUMEN
The clinical features of infection with human herpesvirus 7 (HHV-7) are not well described. Exanthem subitum is the only illness that is confirmed to be caused by HHV-7. We report two children who had exanthem subitum associated with central nervous system manifestations. Two strains of HHV-7 were isolated sequentially from peripheral blood mononuclear cells and saliva of the some child who had exanthem subitum complicated with acute hemiplegia in childhood. Two strains were confirmed to be HHV-7 by means of monoclonal antibodies to human herpesvirus 6 (HHV-6) and HHV-7, polymerase chain reaction, and DNA analysis. During the convalescent period, the antibody titer to HHV-7 rose from less than 1:10 to 1:320, whereas the antibody titer to HHV-6 remained less than 1:10. Another child with exanthem subitum complicated by acute hemiplegia had serologic evidence of primary HHV-7 infection. These two cases demonstrate a new relationship between HHV-7 and central nervous system symptoms.
Asunto(s)
Encefalopatías/virología , Infecciones por Herpesviridae/patología , Herpesvirus Humano 7 , Anticuerpos Antivirales/análisis , Encefalopatías/patología , ADN Viral/análisis , Epilepsia Generalizada/virología , Epilepsia Tónico-Clónica/virología , Exantema Súbito/patología , Exantema Súbito/virología , Femenino , Hemiplejía/virología , Herpesvirus Humano 6/genética , Herpesvirus Humano 6/inmunología , Herpesvirus Humano 6/aislamiento & purificación , Herpesvirus Humano 7/genética , Herpesvirus Humano 7/inmunología , Herpesvirus Humano 7/aislamiento & purificación , Humanos , Lactante , Leucocitos Mononucleares/virología , Reacción en Cadena de la Polimerasa , Saliva/virologíaRESUMEN
An actinomycete strain SC-4710, a new soil isolate, was found to produce a new macrolide antibiotic, PC-766B. Chemotaxonomic analysis of the producing organism revealed that the cells of SC-4710 had type IV cell wall, type A whole cell sugar pattern, type PII phospholipids, menaquinone MK-8(H4), cellular fatty acids comprising straight-chain saturated, unsaturated and tuberculostearic acids, and mycolic acids. The strain was identified as Nocardia brasiliensis (Lindenberg) Pinoy. The antibiotic, PC-766B, was active against Gram-positive bacteria, and some fungi and yeasts, but inactive against Gram-negative bacteria. It also showed antitumor activity against murine tumor cells in vitro and in vivo, and a weak inhibitory activity against Na+, K(+)-ATPase in vitro.