RESUMEN
Papulo-purpuric gloves and socks syndrome, a typical maculopurpuric exanthema limited to hands and feet, mainly associated with Parvovirus B19 infection, has not, until now been reported in the literature in relation to pediatric cases in Italy. The case of a 10-year old girl with all the characteristics of the papulo-purpuric gloves and socks syndrome, as well as being IgM positive anti Parvovirus B19, is reported. This syndrome, despite its spontaneous remission, should be included in the differential diagnosis of maculopurpuric exanthemas.
Asunto(s)
Eritema Infeccioso/diagnóstico , Exantema/virología , Dermatosis del Pie/virología , Dermatosis de la Mano/virología , Niño , Femenino , Humanos , SíndromeRESUMEN
With the aim of isolating the Tumor Liberated Protein (TLP) gene, reverse transcription-polymerase chain reaction (RT-PCR) was used to isolate a approximately equal to 500 bp fragment containing a putative TLP cDNA sequence. Total RNAs were extracted from several cell lines with RNAzol B reagent (Tel-Test, Inc) and reverse transcribed using the Reverse Transcription System (Promega). PCR was carried out for 35 cycles (1 minute at 95 degrees C, 2 minutes at 40 degrees C and 1 minute at 72 degrees C) using an upstream degenerate oligonucleotide, ACN AAY AAR GAR GCN TCN ATG TG, which corresponds to the amino acid sequence T N K E A S I, and random hexamers as the downstream primer. PCR products were electrophoresed on a 1% agarose gel containing ethidium bromide. The PCR products were cloned in the pGEM-T easy vector (PROMEGA) and the resulting plasmid clones were sequenced with the chain termination method using the Applied Biosystems model 373A DNA sequencer. A putative open reading frame was deducted. The results obtained can be considered as preliminary data that will require more investigation in order to confirm them. We propose to continue the studies to verify that TLP could be a diagnostic marker in human cancer.
Asunto(s)
Antígenos de Neoplasias/genética , ADN Complementario/genética , Secuencia de Aminoácidos , Antígenos de Neoplasias/química , Secuencia de Bases , ADN Complementario/química , ADN Complementario/aislamiento & purificación , Humanos , Neoplasias Pulmonares/química , Neoplasias Pulmonares/genética , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Células Tumorales CultivadasRESUMEN
Our earlier studies led to the purification of a similar antigen from several types of human tumours which had the following properties: It provoked a delayed reaction in certain cancer patients which could be demonstrated both in vivo and in vitro (1). In contrast, healthy individuals did not react to this antigen. Subsequent studies led to a higher degree of purity of tumour antigen which enabled a partial sequence (2). The eight amino acid sequence obtained led to the synthesis of the corresponding peptide. This synthetic peptide was then used to raise specific antibodies in rabbits. The availability of such antibodies enabled to undertake a range of studies. Those investigations revealed that the antibodies were tumour specific because they reacted only with certain types of tumours but not with normal cells nor with other types of tumour tissues. We decided to name this tumour antigen as TLP for tumour liberated protein.
Asunto(s)
Antígenos de Neoplasias/aislamiento & purificación , Biomarcadores de Tumor/análisis , Neoplasias/inmunología , Adenocarcinoma/sangre , Adenocarcinoma/inmunología , Secuencia de Aminoácidos , Animales , Antígenos de Neoplasias/inmunología , Neoplasias Óseas/inmunología , Neoplasias de la Mama/sangre , Neoplasias de la Mama/inmunología , Carcinoma de Pulmón de Células no Pequeñas/sangre , Carcinoma de Pulmón de Células no Pequeñas/inmunología , Carcinoma de Células Escamosas/sangre , Carcinoma de Células Escamosas/inmunología , Femenino , Humanos , Neoplasias Pulmonares/sangre , Neoplasias Pulmonares/inmunología , Masculino , Neoplasias/sangre , Neoplasias/diagnóstico , Neoplasias/terapia , Especificidad de Órganos , Osteosarcoma/inmunología , Fragmentos de Péptidos/inmunología , Conejos , Timoma/sangre , Timoma/inmunología , Neoplasias del Timo/sangre , Neoplasias del Timo/inmunología , Neoplasias Urogenitales/sangre , Neoplasias Urogenitales/inmunologíaRESUMEN
The aim of this study was to evaluate efficacy of highly active antiretroviral therapy (HAART) in 64 HIV-1 positive treatment-naive and previously treated patients, with different viral load at baseline. The HAART regimen consisted of one protease inhibitor and two reverse-transcriptase inhibitors. Plasma RNA viral load was measured by RT-PCR (Roche Amplicor Monitor kit) at enrolment and at months 3, 6, 9, and 12 of follow-up. The viral load fell below the threshold of 200 copies/ml in 46.9% of patients at month 6 and this result lasted up to month 12 of follow-up in 42.2% of patients. In these patients the CD4+ cell count increased from a baseline with a median of 194 cells/mmc at month 12. Treatment failure occurred in 35.9% of patients and the proportion was higher among previously treated patients. 7 patients stopped therapy because of poor compliance and 5 because of adverse drug effects. Also in our cases the HAART regimen showed more efficacy in treatment-naive patients, whereas baseline viral load >1x105 was the cause of less efficacy of therapy.
RESUMEN
The role of serum tumor markers in lung cancer has not been defined. It is widely believed that almost all neoplasms produce at least a few substances, but the identity of most of the known substances has not been correlated with a positive role in either the diagnosis or prognosis of patients with these neoplasms. We observed a significant immunogenic response after injecting patients with an antigen extracted from tumor-liberated particles (TLP). A specific ELISA test for TLP performed on sera from patients with lung cancer was reported recently. Based on this information, we developed a polyclonal antibody anti-TLP and screened several cell lines and tumor specimens. We concluded that TLP is a cytoplasmatic antigen specific for epithelial derived neoplasms because it was not detected in non neoplastic tissue cells, in neuroendocrine-tumors or in mesenchymal-derived tumors and normal cells. In addition, the finding that TLP is detectable in neoplasms and tumoral cell lines with common embryologic features seems to suggest that TLP might be an "oncofetal antigen". However, these preliminary observations need confirmation by additional studies using larger numbers of patients and tumor types.
Asunto(s)
Antígenos de Neoplasias/análisis , Neoplasias Pulmonares/patología , Proteínas de Neoplasias/análisis , Secuencia de Aminoácidos , Animales , Anticuerpos , Especificidad de Anticuerpos , Antígenos de Neoplasias/inmunología , Western Blotting , Ensayo de Inmunoadsorción Enzimática , Humanos , Inmunohistoquímica , Neoplasias Pulmonares/cirugía , Proteínas de Neoplasias/inmunología , Fragmentos de Péptidos/inmunología , Conejos , Estudios RetrospectivosRESUMEN
The total and fractioned titres of circulating anticytomegalovirus (CMV) and antiherpesvirus (HSV) antibodies were evaluated in 63 idiopathic ulcerative proctocolitis patients in follow-up. The population was disaggregated applying criteria related to the stage of the disease: duration, extension, number of observed relapses over the follow-up. It was noted that just the anti-HSV antibodies were correlated in a statistically significant way to the extension (p less than 0.025) and duration (p less than 0.025) of the disease. We believe that this behaviour is a manifestation of the disease and that the antiHSV titration may be included in a score of evaluation during the follow-up.
Asunto(s)
Anticuerpos Antivirales/sangre , Colitis Ulcerosa/sangre , Citomegalovirus/inmunología , Simplexvirus/inmunología , Adulto , Femenino , Estudios de Seguimiento , Humanos , MasculinoRESUMEN
A group of 484 patients having regular haemodialysis was tested for the presence of antibodies to the human immunodeficiency virus (HIV). With a commercial enzyme-linked immunoassay kit, the serum of 17 appeared positive. When these 17 samples were retested by a different method, however, none was found to contain antibodies to the virus. Furthermore, evaluation of the clinical state of these 17 patients for the presence of any prodromal symptoms associated with the acquired immune deficiency syndrome was negative. It is therefore suggested that patients having regular haemodialysis are presently at low risk of contracting infection by HIV. By contrast, 81% of these patients had antibodies to cytomegalovirus.
Asunto(s)
Síndrome de Inmunodeficiencia Adquirida/epidemiología , Anticuerpos Antivirales/análisis , VIH/inmunología , Diálisis Renal , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Niño , Preescolar , Citomegalovirus/inmunología , Ensayo de Inmunoadsorción Enzimática , Femenino , Anticuerpos Anti-VIH , Humanos , Masculino , Persona de Mediana Edad , RiesgoRESUMEN
From January 1979 to March 1979, 341 young children from the metropolitan area of Naples, Italy, were hospitalized with respiratory virus disease. Diagnosis of patients made from virus isolation and seroconversion indicate that the respiratory syncytial virus was a primary cause of this acute respiratory syndrome.
Asunto(s)
Virus Sincitiales Respiratorios/aislamiento & purificación , Infecciones del Sistema Respiratorio/etiología , Infecciones por Respirovirus/etiología , Enfermedad Aguda , Antígenos Virales/análisis , Preescolar , Humanos , Lactante , Recién Nacido , Virus Sincitiales Respiratorios/inmunología , SíndromeRESUMEN
Significant immunogenic properties were observed with an antigen extracted from tumor-liberated particles (TLP). Several different tumors were used to obtain antigens by such cell analysis and purification procedures as: gel filtration on Sephadex G-100, ion-exchange chromatography, affinity chromatography, and gel filtration on Sephadex G-200. The molecular weight determined on SDS-PAGE was about 214,000 daltons. In vivo, TLP yields delayed hypersensitivity reactions both in autologous and homologous hosts. TLP does not seem to have any nonspecific mitogenic activity, but it does have specific mitogenic activity, since lymphocyte blastogenesis (TLP-induced in presensitized patients) could mean its ability when intradermally inoculated to sensitize a lymphocytic clone.
Asunto(s)
Antígenos de Neoplasias/aislamiento & purificación , Hipersensibilidad Tardía/etiología , Adulto , Anciano , Antígenos de Neoplasias/administración & dosificación , Antígenos de Neoplasias/inmunología , Niño , Femenino , Humanos , Inmunidad Celular , Inmunoterapia , Activación de Linfocitos , Linfocitos/inmunología , Linfocitos/patología , Masculino , Persona de Mediana Edad , Mitosis , Peso Molecular , Neoplasias/terapia , Pruebas CutáneasRESUMEN
A screening test for detecting antibodies to HSV-TAA was performed on 26 sera, from urinary bladder carcinoma patients and on 30 sera from prostatic carcinoma patients. Positive results were found in 50% of the urinary bladder cancer sera and in 60% of the prostatic carcinoma sera. As control 10/11 sera from benign prostatic hypertrophy and 4/4 sera from renal pelvis or ureter neoplasias (tumors never previously associated to HSV) were negative before and after surgery. The only one positive of the former group became negative after surgical treatment. The TAF-test can therefore be a meaningful test for monitoring antibody levels in the sera of patients before, during or after the treatment, in order to evaluate the presence of metastases or the success of a therapy.
Asunto(s)
Anticuerpos Antineoplásicos/análisis , Neoplasias Urológicas/inmunología , Adulto , Anciano , Animales , Epítopos/inmunología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estadificación de Neoplasias , Hiperplasia Prostática/inmunología , Neoplasias de la Próstata/inmunología , Neoplasias de la Próstata/terapia , Pruebas Serológicas , Simplexvirus/inmunología , Factores de Tiempo , Infecciones Tumorales por Virus/inmunología , Neoplasias de la Vejiga Urinaria/inmunología , Neoplasias de la Vejiga Urinaria/terapia , Neoplasias Urológicas/terapiaAsunto(s)
Antígenos de Neoplasias/análisis , Antígenos Virales/análisis , Simplexvirus/inmunología , Adenocarcinoma/inmunología , Animales , Antígenos Virales de Tumores , Carcinoma de Células Escamosas/inmunología , Carcinoma de Células Transicionales/inmunología , Neoplasias del Colon/inmunología , Ensayo de Inmunoadsorción Enzimática , Femenino , Cobayas , Neoplasias de Cabeza y Cuello/inmunología , Humanos , Neoplasias Renales/inmunología , Neoplasias del Recto/inmunología , Neoplasias de la Vejiga Urinaria/inmunología , Neoplasias del Cuello Uterino/inmunologíaRESUMEN
An ELISA has been developed to detect specific antibodies for HSV-TAA in sera of patients with head, neck and urogenital tract carcinomas (Cancer, 45:1980). Further studies have shown that 64/800 controls (8.12%), i.e. healthy people, are positive against 312/425 patients with herpes associated tumors (73.41%). People with herpes recurrens show 18% positivity, precancerous lesions 44% and other cancers 6%. Immunodepression, chemo- and radio-therapy play an inhibitory role on the ELISA positivity for TAA. Radical surgery of the HSV-TAA positive cancers results in lack of specific antibody whereas relapse or metastasis of the tumor yields positive results. The reproducibility of the test is very good and the standard error is low (1.0655). The conclusions allow us to foresee the use of this ELISA for early detection of the HSV-TAA antibodies in certain human carcinomas.
Asunto(s)
Antígenos de Neoplasias/análisis , Antígenos Virales/análisis , Simplexvirus/inmunología , Animales , Ensayo de Inmunoadsorción Enzimática , CobayasRESUMEN
In the present study, we attempted to purify herpes simplex virus (HSV) tumor associated antigen(s) (TAA) extracted from human kidney carcinoma. Trypsinized human tumor cells were sonicated for 9 minutes and clarified at 100,000 x g for 1 hour; the supernate yielded 70% of detectable TAA as determined by means of quantitative absorption with specific antisera. The supernate used as source of soluble HSV-TAA was concentrated and the pellet was resuspended in 0.02 M tris, pH 7.2, and purified by means of filtration on Sephadex G-100 followed by chromatography on DEAE Sephadex A-50 and then affinity chromatography on concanavalin A (Con A) sepharose. The TAA bound to Con A sepharose was eluted by 0.5 M of alpha-CH3D-mannoside (alpha-MM) and behaved as a glycoprotein. The molecular weight determined on SDS-PAGE was about 70,000 daltons in relation to standard marker proteins. This antigen reacted in complement fixing tests with hyperimmune guinea pig sera as well as with certain human squamous cancer sera. As a control we used a human kidney carcinoma which showed no complement fixing activity in any of the procedural steps, and as control sera, guinea pig sera prepared by inoculation of uninfected guinea pig cells.
Asunto(s)
Adenocarcinoma/inmunología , Antígenos Virales/aislamiento & purificación , Neoplasias Renales/inmunología , Sarcoma/inmunología , Simplexvirus/inmunología , Cromatografía Liquida , Pruebas de Fijación del Complemento , Humanos , Masculino , Persona de Mediana EdadRESUMEN
The Authors report a study on a group of 47 patients affected by serositis (pleuritis, pericarditis, pleuro-pericarditis) of suspected viral origin. Two serum samples were taken from each patient at different times and were tested with many viral antigens by the complement fixation technique. The discussion is carried out on the lines followed by the majority of people in giving importance to their viral etiology and the viruses involved as agents of serositis are reported. In the present study 18 patients (38%) showed a significant rise of antibodies for viral antigens and therefore their disease might be due to viral etiology: 5 influenza viruses, 1 parainfluenza, 1 coxsackie, 1 RSV, 3 mumps virus, 5 CMV, 1 adenovirus.
Asunto(s)
Pericarditis/etiología , Pleuresia/etiología , Virosis/complicaciones , Adolescente , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Pericarditis/diagnóstico , Pleuresia/diagnósticoRESUMEN
The antibodies against herpes simplex virus (HSV) tumor-associated antigen (TAA) in human sera were investigated by the enzyme-linked immunosorbent assay (ELISA). By combining the findings of antibody levels in many human sera, a significant level of discrimination between normal and cancer sera could be reached. Thus, 57 of 74 pathological sera had increased levels of antibodies against HSV-TAA antigen as compared to 26 of 512 normal sera. A significant correlation between the ELISA values and complement fixing (CF) and radioimmunoassay (RIA) values was also found. The results suggest that serodiagnosis with the ELISA test may therefore be of some use in the detection of specific antibodies against HSV-TAA antigen in sera of cancer patients.
Asunto(s)
Antígenos de Neoplasias/análisis , Antígenos Virales/análisis , Ensayo de Inmunoadsorción Enzimática , Técnicas para Inmunoenzimas , Simplexvirus/inmunología , Pruebas de Fijación del Complemento , Humanos , Sueros Inmunes , RadioinmunoensayoRESUMEN
The latest vaccines for Respiratory Syncytial Virus (RSV) are reviewed. A ts-1 variant is being studied in the attempt to complete the attenuation of the vaccine strain. Further investigations of live RSV vaccine administered parenterally indicate that a target population over 6 months of age could benefit soon of this preparation.