RESUMEN
The activity of adapting and introducing the FTA-carriers for the storage of blood samples of relatives of missing service men for the purpose of using them in the consequent identification measures in respect to non-identified killed persons has been conducted in the Russian Federation within the framework of identification of service men killed in the Chechen territory during the 1994-1996 armed conflict. The article generalizes the experience of practical usage of FTA-cards as an element in the technological sequence of the molecular-genetic identification expertise. Methods related with application of FTA-technologies were tested; the efficiency of polymerized chain reaction was evaluated by using the matrix of immobilized DNA for hypo variable segments HVS-1 and HVS-2 as well as for the D-loop of mitochondrial DNA and for chromosomal STR-locuses D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317 and D7S820.
Asunto(s)
Análisis Químico de la Sangre/normas , Recolección de Muestras de Sangre/instrumentación , Cadáver , Medicina Legal , Secuencia de Bases , Análisis Químico de la Sangre/métodos , Dermatoglifia del ADN , Diseño de Equipo , Humanos , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Federación de Rusia , GuerraRESUMEN
From 1993 till 1999 6 patients with aneurysmointestinal fistulas complicated by bleedings were operated. In 4 cases the aneurysmointestinal fistulas were primary, in 2 cases--secondary. Gastroduodenoscopy, ultrasonic examination of abdominal organs, retroperitoneal space and duplex scanning of main vessels were used. Correct diagnosis before operation was made in 5 patients. The following operations on the vessels were performed: suturing of defect of central anastomosis (1), aortobifemoral grafting (1), abdominal aorta grafting (2), suturing of aortal defect (1), resection of peripheral aneurysm (1). Thrombosis of prosthesis branch (1) and suppuration of prosthesis bed with arrhosive bleeding (2) were early postoperative complications. Immediate postoperative lethality was 33.3%. Long-term results are followed in 3 patients. There were no repeated signs of graft infection in remote period. Basic methods of clinical and instrumental examination including ultrasound permit to make correct diagnosis before operation. Active surgical policy saves the life in the majority of patients, but it is necessary to use carefully the synthetic grafts for arterial vessels repair.
Asunto(s)
Aneurisma de la Aorta Abdominal/complicaciones , Hemorragia Gastrointestinal/etiología , Anciano , Aneurisma de la Aorta Abdominal/diagnóstico , Aneurisma de la Aorta Abdominal/cirugía , Prótesis Vascular , Estudios de Seguimiento , Hemorragia Gastrointestinal/diagnóstico , Hemorragia Gastrointestinal/cirugía , Humanos , Fístula Intestinal/complicaciones , Fístula Intestinal/diagnóstico , Fístula Intestinal/cirugía , Masculino , Persona de Mediana Edad , Factores de TiempoRESUMEN
The combined treatment of 53 patients with colonic cancer, using neoadjuvant intraarterial polychemotherapy (IAPCT), was conducted. The trustworthy lowering of the catheterized artery thrombosis occurrence rate, the tumor recurrence-by 7%, distant metastases-by 13.9%, two-year survival increase-by 15.5% was promoted by verapamil application as a IAPCT modifier.