RESUMEN
Communication between individuals via molecules, termed chemosignaling, is widespread among animal and plant species. However, we lack knowledge on the specific functions of the substances involved for most systems. The femoral gland is an organ that secretes a waxy substance involved in chemical communication in lizards. Although the lipids and volatile substances secreted by the femoral glands have been investigated in several biochemical studies, the protein composition and functions of secretions remain completely unknown. Applying a proteomic approach, we provide the first attempt to comprehensively characterize the protein composition of femoral gland secretions from the Galápagos marine iguana. Using samples from several organs, the marine iguana proteome was assembled by next-generation sequencing and MS, resulting in 7513 proteins. Of these, 4305 proteins were present in the femoral gland, including keratins, small serum proteins, and fatty acid-binding proteins. Surprisingly, no proteins with discernible roles in partner recognition or inter-species communication could be identified. However, we did find several proteins with direct associations to the innate immune system, including lysozyme C, antileukoproteinase (ALP), pulmonary surfactant protein (SFTPD), and galectin (LGALS1) suggesting that the femoral glands function as an important barrier to infection. Furthermore, we report several novel anti-microbial peptides from the femoral glands that show similar action against Escherichia coli and Bacillus subtilis such as oncocin, a peptide known for its effectiveness against Gram-negative pathogens. This proteomics data set is a valuable resource for future functional protein analysis and demonstrates that femoral gland secretions also perform functions of the innate immune system.
Asunto(s)
Antiinfecciosos/metabolismo , Antiinfecciosos/farmacología , Iguanas/metabolismo , Sistema Inmunológico/metabolismo , Inmunidad Innata , Proteoma/metabolismo , Transcriptoma , Animales , Apoproteínas/genética , Apoproteínas/metabolismo , Bacillus subtilis/efectos de los fármacos , Encéfalo/metabolismo , Factores Quimiotácticos/genética , Factores Quimiotácticos/metabolismo , Ecuador , Endopeptidasas/genética , Endopeptidasas/metabolismo , Escherichia coli/efectos de los fármacos , Galectinas/genética , Galectinas/metabolismo , Corazón/fisiología , Secuenciación de Nucleótidos de Alto Rendimiento , Humanos , Iguanas/genética , Iguanas/inmunología , Inmunidad Innata/genética , Pulmón/metabolismo , Muramidasa/genética , Muramidasa/metabolismo , Músculos/metabolismo , Miocardio/metabolismo , Especificidad de Órganos , Proteoma/genética , Proteoma/inmunología , Proteómica , Proteínas Asociadas a Surfactante Pulmonar/genética , Proteínas Asociadas a Surfactante Pulmonar/metabolismo , Piel/metabolismo , Espectrometría de Masas en Tándem , Transcriptoma/genéticaRESUMEN
The lactic acid bacterium Lactobacillus kunkeei has been described as an inhabitant of fructose-rich niches. Here we report on the genome sequence of L. kunkeei EFB6, which has been isolated from a honeybee larva infected with European foulbrood. The draft genome comprises 1,566,851 bp and 1,417 predicted protein-encoding genes.